lentiviral vlp stock  (Worthington Biochemical)


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  • 85
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    ls002004
    Price:
    33
    Size:
    5 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9003.98.9
    Buy from Supplier


    Structured Review

    Worthington Biochemical lentiviral vlp stock
    Nullbasic does not inhibit the production of <t>lentiviral</t> VLPs. (a) Schematic diagrams of the three <t>VLP</t> expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/lentiviral vlp stock/product/Worthington Biochemical
    Average 85 stars, based on 897 article reviews
    Price from $9.99 to $1999.99
    lentiviral vlp stock - by Bioz Stars, 2020-05
    85/100 stars

    Images

    1) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins
    Figure Legend Snippet: Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins

    Techniques Used: Expressing, Plasmid Preparation

    Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant
    Figure Legend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Techniques Used: Purification, Activity Assay

    2) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced
    Figure Legend Snippet: Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced

    Techniques Used: Infection, Produced

    HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was
    Figure Legend Snippet: HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was

    Techniques Used: Expressing, Infection

    HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were
    Figure Legend Snippet: HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were

    Techniques Used: Expressing, Infection

    3) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic inhibits reverse transcription of lentiviral VLPs.  (a)  Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant
    Figure Legend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Techniques Used: Purification, Activity Assay

    Related Articles

    In Vitro:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Positive Control:

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Blocking Assay:

    Article Title: Extracellular traps are associated with human and mouse neutrophil and macrophage mediated killing of larval Strongyloides stercoralis
    Article Snippet: .. Treatment with DNase I eliminated the presence of released DNA, but did not block killing of the larvae by mouse neutrophils and macrophages in vitro. .. This observation suggests that in contrast to human neutrophils and macrophages, mouse cells do not require ET formation in vitro to kill the worms.

    Concentration Assay:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    Article Title: STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells
    Article Snippet: .. Concentration ranges for DNase I were determined empirically for each lot and cell type by titrating DNase I for its ability to digest CR-C as the positive control but not regions previously found to be resistant to DNase I (e.g., +6.3 region). .. Following purification of the digested DNA, PCR was performed across the Pdcd1 locus using a set of 59 primer pairs ( ).

    Incubation:

    Article Title: Epigenetic Control of Cell Cycle-Dependent Histone Gene Expression Is a Principal Component of the Abbreviated Pluripotent Cell Cycle
    Article Snippet: .. Nuclei were then resuspended in RSB buffer supplemented with 1 mM CaCl2 and incubated with increasing concentrations of DNase I (DPRF; Worthington Biochemical Corporation, Lakewood, NJ) for 10 min at room temperature with gentle agitation. ..

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: .. DNase I (Worthington) diluted in DNase I dilution buffer (2.5 mM MgCl2 , 0.5 mM CaCl2 , 10 mM Tris-HCl, pH 7.6, 0.1 mg/ml BSA) to a final concentration of 0.0625 μg/ml was incubated with reaction mixtures for 1 min. ..

    other:

    Article Title: Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment Site DNA
    Article Snippet: The region is dA+dT rich, and protection seen from positions +126 to +129 is subtle because DNase I does not cleave DNA efficiently in this region.

    Article Title: Structural and functional conservation at the boundaries of the chicken ?-globin domain
    Article Snippet: The DNase I HS, 3′HS, was detected by digestion of nuclei with increasing amounts of DNase I as above followed by digestion of extracted DNA with Kpn I and probing with i112 (1000 bp; Kpn I –Pst I at 23.3–24.3 map units).