lentiviral vlp stock  (Worthington Biochemical)


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  • 85
    Name:
    Deoxyribonuclease I
    Description:
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    Catalog Number:
    ls002004
    Price:
    33
    Size:
    5 mg
    Source:
    Bovine Pancreas
    Cas Number:
    9003.98.9
    Buy from Supplier


    Structured Review

    Worthington Biochemical lentiviral vlp stock
    Nullbasic does not inhibit the production of <t>lentiviral</t> VLPs. (a) Schematic diagrams of the three <t>VLP</t> expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins
    Chromatographically purified A lyophilized powder with glycine as a stabilizer
    https://www.bioz.com/result/lentiviral vlp stock/product/Worthington Biochemical
    Average 85 stars, based on 1101 article reviews
    Price from $9.99 to $1999.99
    lentiviral vlp stock - by Bioz Stars, 2020-08
    85/100 stars

    Images

    1) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins
    Figure Legend Snippet: Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins

    Techniques Used: Expressing, Plasmid Preparation

    Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant
    Figure Legend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Techniques Used: Purification, Activity Assay

    2) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced
    Figure Legend Snippet: Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced

    Techniques Used: Infection, Produced

    HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was
    Figure Legend Snippet: HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was

    Techniques Used: Expressing, Infection

    HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were
    Figure Legend Snippet: HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were

    Techniques Used: Expressing, Infection

    3) Product Images from "A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells"

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    Journal: Human Gene Therapy

    doi: 10.1089/hum.2012.176

    Nullbasic inhibits reverse transcription of lentiviral VLPs.  (a)  Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant
    Figure Legend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Techniques Used: Purification, Activity Assay

    Related Articles

    Irradiation:

    Article Title: Characterization of the survival motor neuron (SMN) promoter provides evidence for complex combinatorial regulation in undifferentiated and differentiated P19 cells
    Article Snippet: .. Specifically, we used DMS, UVC irradiation and DNase I as DNA-modifying agents to map single-strand DNA breaks, comparing in vitro (naked DNA) and in vivo (living cells) footprints. .. In vivo footprints corresponding to nt −46 to +125 encompassing the minimal core promoter are presented in and summarized in .

    In Vivo:

    Article Title: Characterization of the survival motor neuron (SMN) promoter provides evidence for complex combinatorial regulation in undifferentiated and differentiated P19 cells
    Article Snippet: .. Specifically, we used DMS, UVC irradiation and DNase I as DNA-modifying agents to map single-strand DNA breaks, comparing in vitro (naked DNA) and in vivo (living cells) footprints. .. In vivo footprints corresponding to nt −46 to +125 encompassing the minimal core promoter are presented in and summarized in .

    In Vitro:

    Article Title: Characterization of the survival motor neuron (SMN) promoter provides evidence for complex combinatorial regulation in undifferentiated and differentiated P19 cells
    Article Snippet: .. Specifically, we used DMS, UVC irradiation and DNase I as DNA-modifying agents to map single-strand DNA breaks, comparing in vitro (naked DNA) and in vivo (living cells) footprints. .. In vivo footprints corresponding to nt −46 to +125 encompassing the minimal core promoter are presented in and summarized in .

    Produced:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Concentration Assay:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Incubation:

    Article Title: Identification of Viral Peptide Fragments for Vaccine Development
    Article Snippet: .. When the random digestion by DNase I is used for some genes, peculiarly a small amount of the full-length gene has been observed even after prolonged incubation. .. In this case, the digestion mixture should be passed through a column with an appropriate molecular weight cut-off to filter out the full-length gene, followed by a Microcon® YM-30 column to accomplish the buffer exchange.

    Article Title: Neutrophil Extracellular Traps Contain Calprotectin, a Cytosolic Protein Complex Involved in Host Defense against Candida albicans
    Article Snippet: .. As controls (i) NETs were mock-digested with nuclease-free RPMI and (ii) unstimulated neutrophils that did not release NETs were washed twice and incubated with RPMI containing 10 U/ml Dnase-1 for 20 min at 37°C. .. Four samples out of 4 wells were pooled, acetone precipitated, solubilized in 120 µl SDS loading buffer and boiled for 3 min. To account for potential protein loss due to proteolytic activity in the samples a complete purification procedure was performed in the presence of protease inhibitor cocktail (Sigma P1860; 1∶200) added to the wells 2 h after stimulation start as described above.

    other:

    Article Title: Transcriptional regulatory logic of the diurnal cycle in the mouse liver
    Article Snippet: Movie S2: Dynamics of DNase I, Pol II and H3K27ac at the Npas2 locus.

    Article Title: Identification of Viral Peptide Fragments for Vaccine Development
    Article Snippet: In the random digestion step, the use of DNase I in the presence of MnCl2 is critical as this protocol will generate DNA fragments of relatively uniform sizes, which facilitates the reassembly step ( 17 , also see Note ).

    Article Title: Neutrophil Extracellular Traps Contain Calprotectin, a Cytosolic Protein Complex Involved in Host Defense against Candida albicans
    Article Snippet: Supernatants were removed, NETs were washed twice with 1 ml RPMI and digested with 500 µl 10 U/ml DNase-1 each.

    Activity Assay:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

    Staining:

    Article Title: Constitutive Nucleosome Depletion and Ordered Factor Assembly at the GRP78 Promoter Revealed by Single Molecule Footprinting
    Article Snippet: .. These were then digested at 37 °C for 15 min using various concentrations of DNase I (Worthington, San Francisco, California, United States) to obtain a suitable range of digestion of genomic DNA as revealed by EtBr staining. .. Digested genomic DNA was purified, redigested by RsaI, resolved on a 1.5% agarose gel, and Southern blotted.

    Recombinant:

    Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
    Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

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  • 99
    Worthington Biochemical dnase i
    Nullbasic inhibits reverse transcription of lentiviral VLPs.  (a)  Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant
    Dnase I, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Worthington Biochemical
    Average 99 stars, based on 431 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-08
    99/100 stars
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    85
    Worthington Biochemical lentiviral vlp stock
    Nullbasic does not inhibit the production of <t>lentiviral</t> VLPs. (a) Schematic diagrams of the three <t>VLP</t> expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins
    Lentiviral Vlp Stock, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentiviral vlp stock/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lentiviral vlp stock - by Bioz Stars, 2020-08
    85/100 stars
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    88
    Worthington Biochemical cap24
    Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various <t>CAp24</t> inoculation amounts, as indicated. Transduced
    Cap24, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cap24/product/Worthington Biochemical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cap24 - by Bioz Stars, 2020-08
    88/100 stars
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    Image Search Results


    Nullbasic inhibits reverse transcription of lentiviral VLPs.  (a)  Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Purification, Activity Assay

    Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: Nullbasic does not inhibit the production of lentiviral VLPs. (a) Schematic diagrams of the three VLP expression plasmids and an overview of the methods used to generate lentiviral VLPs. pCMVΔR8.91 plasmid expresses structural and regulatory proteins

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Expressing, Plasmid Preparation

    Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: Nullbasic inhibits reverse transcription of lentiviral VLPs. (a) Using partially purified VLPs, ERT assays were performed with equivalent amounts of RT activity determined in a homopolymeric primer:template RT assay. Briefly, DNase I-treated VLP supernatant

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Purification, Activity Assay

    Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: Nullbasic diminishes the infectivity of lentiviral VLPs. Nullbasic-EGFP (NB-EGFP) or control EGFP VLPs, produced as per , were transduced into (a) Jurkat, (b) HEK293T, or (c) HeLa cells at various CAp24 inoculation amounts, as indicated. Transduced

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Infection, Produced

    HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: HIV replication is inhibited in activated human CD4 + primary cells expressing NB-ZsGreen1. (a) The two CD4 + populations described in were infected with HIV-1 89.6 containing 20 ng of CAp24 and grown for 21 days. Cell-free supernatant was

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Expressing, Infection

    HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were

    Journal: Human Gene Therapy

    Article Title: A Mutant Tat Protein Provides Strong Protection from HIV-1 Infection in Human CD4+ T Cells

    doi: 10.1089/hum.2012.176

    Figure Lengend Snippet: HIV replication is significantly delayed in Jurkat cell populations expressing Nullbasic-EGFP. The two Jurkat cell lines shown in were infected with HIV-1 pNL4-3 containing 20 ng of CAp24 and grown for 4 weeks. (a) The cell cultures were

    Article Snippet: Lentiviral VLP stock containing 1 μg of CAp24 was treated with DNase I (Worthington Biochemicals, Lakewood, NUJ) and pelleted through 1×phosphate-buffered saline (PBS) containing 20% (v/v) sucrose at 100,000× g for 2 hr, using an SW 41 Ti rotor (Beckman Coulter, Brea, CA).

    Techniques: Expressing, Infection