monarch rna cleanup binding buffer  (New England Biolabs)


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    New England Biolabs monarch rna cleanup binding buffer
    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total <t>RNA</t> was subjected to <t>m6A</t> <t>RIP,</t> followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.
    Monarch Rna Cleanup Binding Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup binding buffer/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup binding buffer - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Aging-Associated Differences in Epitranscriptomic m6A Regulation in Response to Acute Cardiac Ischemia/Reperfusion Injury in Female Mice"

    Article Title: Aging-Associated Differences in Epitranscriptomic m6A Regulation in Response to Acute Cardiac Ischemia/Reperfusion Injury in Female Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.654316

    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: In Vitro, Quantitative RT-PCR

    monarch rna cleanup binding buffer  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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    Structured Review

    New England Biolabs monarch rna cleanup binding buffer
    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total <t>RNA</t> was subjected to <t>m6A</t> <t>RIP,</t> followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.
    Monarch Rna Cleanup Binding Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup binding buffer/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup binding buffer - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Aging-Associated Differences in Epitranscriptomic m6A Regulation in Response to Acute Cardiac Ischemia/Reperfusion Injury in Female Mice"

    Article Title: Aging-Associated Differences in Epitranscriptomic m6A Regulation in Response to Acute Cardiac Ischemia/Reperfusion Injury in Female Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2021.654316

    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: In Vitro, Quantitative RT-PCR

    monarch rna cleanup binding buffer  (New England Biolabs)


    Bioz Verified Symbol New England Biolabs is a verified supplier
    Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 92

    Structured Review

    New England Biolabs monarch rna cleanup binding buffer
    Monarch Rna Cleanup Binding Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup binding buffer/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup binding buffer - by Bioz Stars, 2024-05
    92/100 stars

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    New England Biolabs monarch rna cleanup binding buffer
    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total <t>RNA</t> was subjected to <t>m6A</t> <t>RIP,</t> followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.
    Monarch Rna Cleanup Binding Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch rna cleanup binding buffer/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch rna cleanup binding buffer - by Bioz Stars, 2024-05
    92/100 stars
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    In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Aging-Associated Differences in Epitranscriptomic m6A Regulation in Response to Acute Cardiac Ischemia/Reperfusion Injury in Female Mice

    doi: 10.3389/fphar.2021.654316

    Figure Lengend Snippet: In vitro hypoxia/reperfusion (iH/R) stress regulates m6A levels of key anti-apoptotic and pro-apoptotic genes. ( A–C ). Ctrl and Mettl3 mofified HL1 cardiomyocytes were subjected to sham or iH/R, and total RNA was subjected to m6A RIP, followed by RT-qPCR using primers for the indicated pro-apoptotic genes (Bax and PTEN) and anti-apoptitic gene (Bcl2). Values are normalized to input. n = 3–5, * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Briefly, 2 µl N6-Methyladenosine Antibody was added to protein G magnetic beads (Thermo Fisher) and incubated at 4°C for 2 h. Following two washes in reaction buffer, the RNA was added to the antibody–bead mixture containing RNasin Plus RNase Inhibitor (Promega) and incubated at 4°C for 2 h. Next, RIP-enriched RNAs were isolated from the antibody-immobilized protein G beads using Monarch RNA cleanup binding buffer from Monarch RNA Cleanup kit (New England Biolabs).

    Techniques: In Vitro, Quantitative RT-PCR