monarch dna cleanup kit  (New England Biolabs)


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    Name:
    Monarch DNA Cleanup Columns 5ug
    Description:
    Monarch DNA Cleanup Columns 5ug 100 columns
    Catalog Number:
    t1034l
    Price:
    130
    Size:
    100 columns
    Category:
    DNA Purification Cartridges Columns
    Buy from Supplier


    Structured Review

    New England Biolabs monarch dna cleanup kit
    Monarch DNA Cleanup Columns 5ug
    Monarch DNA Cleanup Columns 5ug 100 columns
    https://www.bioz.com/result/monarch dna cleanup kit/product/New England Biolabs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    monarch dna cleanup kit - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA"

    Article Title: Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA

    Journal: Journal of Virology

    doi: 10.1128/JVI.01328-19

    RcGTA gp1 in vitro DNA binding. (A) Representative agarose gel (0.8%, wt/vol) showing the stated concentrations of gp1 protein binding to DNA in an electrophoretic mobility shift assay (EMSA). The locations of unbound and shifted DNA are annotated. Substrate DNA in the assay shown is a 1.4-kbp PCR amplification of an arbitrarily chosen region flanking the rcc01398 gene from R. capsulatus (amplified using rcc01398 forward and reverse primers [ Table 3 ]). Bioline HyperLadder 1kb DNA marker is shown for size comparison (lane M). (B) Quantification of EMSAs by band intensity analysis. Data shown are the average results of two EMSAs carried out independently in time and with different DNA substrates (flanking the rcc01397 and rcc01398 genes). Individual data points are plotted as well as the mean line.
    Figure Legend Snippet: RcGTA gp1 in vitro DNA binding. (A) Representative agarose gel (0.8%, wt/vol) showing the stated concentrations of gp1 protein binding to DNA in an electrophoretic mobility shift assay (EMSA). The locations of unbound and shifted DNA are annotated. Substrate DNA in the assay shown is a 1.4-kbp PCR amplification of an arbitrarily chosen region flanking the rcc01398 gene from R. capsulatus (amplified using rcc01398 forward and reverse primers [ Table 3 ]). Bioline HyperLadder 1kb DNA marker is shown for size comparison (lane M). (B) Quantification of EMSAs by band intensity analysis. Data shown are the average results of two EMSAs carried out independently in time and with different DNA substrates (flanking the rcc01397 and rcc01398 genes). Individual data points are plotted as well as the mean line.

    Techniques Used: In Vitro, Binding Assay, Agarose Gel Electrophoresis, Protein Binding, Electrophoretic Mobility Shift Assay, Polymerase Chain Reaction, Amplification, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA). .. Genotyping was performed using an Infinium Global Screening Array-24 BeadChip (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions, at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands.

    Article Title: Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA
    Article Snippet: .. DNA substrates were prepared by PCR amplification with oligonucleotides indicated in and cleaned with a Monarch DNA cleanup kit (NEB). .. Ten-microliter electrophoretic mobility shift assay (EMSA) mixtures contained 100 ng of DNA, binding buffer based on reference [25 mM HEPES, 50 mM K-glutamate, 1 mM dithiothreitol, 0.05% Triton X-100, 4% glycerol, 1 μg poly(dI-dC), pH 8.0], and purified protein at stated concentrations.

    Amplification:

    Article Title: Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA
    Article Snippet: .. DNA substrates were prepared by PCR amplification with oligonucleotides indicated in and cleaned with a Monarch DNA cleanup kit (NEB). .. Ten-microliter electrophoretic mobility shift assay (EMSA) mixtures contained 100 ng of DNA, binding buffer based on reference [25 mM HEPES, 50 mM K-glutamate, 1 mM dithiothreitol, 0.05% Triton X-100, 4% glycerol, 1 μg poly(dI-dC), pH 8.0], and purified protein at stated concentrations.

    Binding Assay:

    Article Title: No longer locally extinct? Tracing the origins of a lion (Panthera leo) living in Gabon
    Article Snippet: .. The digest was centrifuged at 6000g for 1 min, after which 500 μl of supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. ( ) then centrifuged through Monarch DNA Cleanup Columns (5 μg) (New England Biolabs Inc. Beverly, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen, Hilden, Germany), then eluted using two washes in 12 μl buffer EB (Qiagen)—each with an incubation of 5 min at 37 °C.

    Article Title: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing
    Article Snippet: .. Digests from methods B and C were centrifuged at 6000 ×G for 1 minute, after which 500 μl supernatant was mixed 1:8 with a binding buffer as detailed in Allentoft et al. [ ], then centrifuged through Monarch DNA Cleanup Columns (New England Biolabs, MA, USA). .. DNA bound to the columns was washed with 800 μl buffer PE (Qiagen), then eluted using 2 washes in 17 μl buffer EB (Qiagen)—each with an incubation for 5 minutes at 37°C.

    Article Title: Genomic variants identified from whole-genome resequencing of indicine cattle breeds from Pakistan
    Article Snippet: .. After the final best fill-in step in gDNA library construction, all gDNA libraries were mixed with Qiagen PB binding buffer (1:5 volume) and cleaned by employing Monarch DNA clean-up columns (New England Biolabs, Massachusetts, USA). .. For washing, size-selected gDNA libraries were first incubated at 37◦C for 5-minutes in 750 μL PE buffer (Qiagen) and finally purified gDNA libraries were eluted using 40 μL Qiagen EB.

    Purification:

    Article Title: Polymorphisms in RAS/RAF/MEK/ERK Pathway Are Associated with Gastric Cancer
    Article Snippet: .. In both cases, genomic DNA was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs (NEB), Ipswich, MA, USA). .. Genotyping was performed using an Infinium Global Screening Array-24 BeadChip (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions, at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands.

    Article Title: Species-specific recruitment of transcription factors dictates toxin expression
    Article Snippet: .. DNA was purified on Monarch DNA Clean Up columns (NEB). ..

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  • 94
    New England Biolabs monarch dna cleanup kit
    RcGTA gp1 in vitro <t>DNA</t> binding. (A) Representative agarose gel (0.8%, wt/vol) showing the stated concentrations of gp1 protein binding to DNA in an electrophoretic mobility shift assay (EMSA). The locations of unbound and shifted DNA are annotated. Substrate DNA in the assay shown is a 1.4-kbp <t>PCR</t> amplification of an arbitrarily chosen region flanking the rcc01398 gene from R. capsulatus (amplified using rcc01398 forward and reverse primers [ Table 3 ]). Bioline HyperLadder 1kb DNA marker is shown for size comparison (lane M). (B) Quantification of EMSAs by band intensity analysis. Data shown are the average results of two EMSAs carried out independently in time and with different DNA substrates (flanking the rcc01397 and rcc01398 genes). Individual data points are plotted as well as the mean line.
    Monarch Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch dna cleanup kit/product/New England Biolabs
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    monarch dna cleanup kit - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    RcGTA gp1 in vitro DNA binding. (A) Representative agarose gel (0.8%, wt/vol) showing the stated concentrations of gp1 protein binding to DNA in an electrophoretic mobility shift assay (EMSA). The locations of unbound and shifted DNA are annotated. Substrate DNA in the assay shown is a 1.4-kbp PCR amplification of an arbitrarily chosen region flanking the rcc01398 gene from R. capsulatus (amplified using rcc01398 forward and reverse primers [ Table 3 ]). Bioline HyperLadder 1kb DNA marker is shown for size comparison (lane M). (B) Quantification of EMSAs by band intensity analysis. Data shown are the average results of two EMSAs carried out independently in time and with different DNA substrates (flanking the rcc01397 and rcc01398 genes). Individual data points are plotted as well as the mean line.

    Journal: Journal of Virology

    Article Title: Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA

    doi: 10.1128/JVI.01328-19

    Figure Lengend Snippet: RcGTA gp1 in vitro DNA binding. (A) Representative agarose gel (0.8%, wt/vol) showing the stated concentrations of gp1 protein binding to DNA in an electrophoretic mobility shift assay (EMSA). The locations of unbound and shifted DNA are annotated. Substrate DNA in the assay shown is a 1.4-kbp PCR amplification of an arbitrarily chosen region flanking the rcc01398 gene from R. capsulatus (amplified using rcc01398 forward and reverse primers [ Table 3 ]). Bioline HyperLadder 1kb DNA marker is shown for size comparison (lane M). (B) Quantification of EMSAs by band intensity analysis. Data shown are the average results of two EMSAs carried out independently in time and with different DNA substrates (flanking the rcc01397 and rcc01398 genes). Individual data points are plotted as well as the mean line.

    Article Snippet: DNA substrates were prepared by PCR amplification with oligonucleotides indicated in and cleaned with a Monarch DNA cleanup kit (NEB).

    Techniques: In Vitro, Binding Assay, Agarose Gel Electrophoresis, Protein Binding, Electrophoretic Mobility Shift Assay, Polymerase Chain Reaction, Amplification, Marker