phospho erk1 t202 y204  (Alomone Labs)


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    Alomone Labs phospho erk1 t202 y204
    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the <t>ERK1/2</t> inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.
    Phospho Erk1 T202 Y204, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho erk1 t202 y204/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho erk1 t202 y204 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages"

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23158603

    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.
    Figure Legend Snippet: Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.

    Techniques Used: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control and 5 mM K + .
    Figure Legend Snippet: Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control and 5 mM K + .

    Techniques Used: Expressing, Derivative Assay, Western Blot

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    Alomone Labs phospho erk1 t202 y204
    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the <t>ERK1/2</t> inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.
    Phospho Erk1 T202 Y204, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho erk1 t202 y204/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho erk1 t202 y204 - by Bioz Stars, 2023-01
    94/100 stars
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    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.

    Journal: International Journal of Molecular Sciences

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages

    doi: 10.3390/ijms23158603

    Figure Lengend Snippet: Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control.

    Article Snippet: Primary antibodies against K Ca 3.1 (rabbit polyclonal), ERK1/2 (rabbit polyclonal), phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (rabbit monoclonal), JNK (rabbit polyclonal), phospho-JNK(Y185) (rabbit polyclonal), c-Jun (rabbit polyclonal), CREB (rabbit polyclonal), phospho-CREB(S133) (rabbit polyclonal), and β-actin (ACTB) (mouse monoclonal) were from Alomone Labs (Jerusalem, Israel), BioLegend (San Diego, CA, USA), R&D Systems (Minneapolis, MN, USA), GeneTex (Alton Pkwy Irvine, CA, USA), ProteinTech (Rosemont, IL, USA), Medical & Biological Laboratories (Nagoya, Japan), and ABclonal (Tokyo, Japan).

    Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control and 5 mM K + .

    Journal: International Journal of Molecular Sciences

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca 2+ -Activated K + Channel K Ca 3.1 in THP-1-Derived M 2 Macrophages

    doi: 10.3390/ijms23158603

    Figure Lengend Snippet: Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p < 0.01 vs. the vehicle control and 5 mM K + .

    Article Snippet: Primary antibodies against K Ca 3.1 (rabbit polyclonal), ERK1/2 (rabbit polyclonal), phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (rabbit monoclonal), JNK (rabbit polyclonal), phospho-JNK(Y185) (rabbit polyclonal), c-Jun (rabbit polyclonal), CREB (rabbit polyclonal), phospho-CREB(S133) (rabbit polyclonal), and β-actin (ACTB) (mouse monoclonal) were from Alomone Labs (Jerusalem, Israel), BioLegend (San Diego, CA, USA), R&D Systems (Minneapolis, MN, USA), GeneTex (Alton Pkwy Irvine, CA, USA), ProteinTech (Rosemont, IL, USA), Medical & Biological Laboratories (Nagoya, Japan), and ABclonal (Tokyo, Japan).

    Techniques: Expressing, Derivative Assay, Western Blot