icilin  (Alomone Labs)


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    Alomone Labs icilin
    Upregulation of autophagy by <t>TRPM8</t> in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM <t>icilin,</t> 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P
    Icilin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icilin/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icilin - by Bioz Stars, 2021-12
    93/100 stars

    Images

    1) Product Images from "Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy"

    Article Title: Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2020.573127

    Upregulation of autophagy by TRPM8 in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM icilin, 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P
    Figure Legend Snippet: Upregulation of autophagy by TRPM8 in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM icilin, 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P

    Techniques Used: Construct, Expressing, Transfection, Western Blot, Multiple Displacement Amplification

    Mechanism of the stimulatory effect of TRPM8 channel function on basal autophagy. (A, B) HeLa cells were incubated with menthol (10 μM) for 48 h. The cells were lysed and assayed by western blot using indicated antibodies (N = 3). (C, D) WB analysis of HeLa cell lysates after treatment with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h (N = 3). (E, F) HEK293 cells were cotransfected with EGFP and wild type TRPM8, mutant, or a control vector. After 48 h of transfection, EGFP-positive cells were selected for recording of TRPM8 channel-mediated current. The number of cells used for current recordings: Vector (6); WT: (10); V976W (12). (G, H) HeLa cells were transfected with the constructs for the expression of wild type or mutant TRPM8. After 48 h of transfection, cells were lysed and subjected to WB analysis using indicated antibodies (N = 3). N represents the number of replicate experiments. EGFP, enhanced green fluorescent protein; Ctrl, control; *P
    Figure Legend Snippet: Mechanism of the stimulatory effect of TRPM8 channel function on basal autophagy. (A, B) HeLa cells were incubated with menthol (10 μM) for 48 h. The cells were lysed and assayed by western blot using indicated antibodies (N = 3). (C, D) WB analysis of HeLa cell lysates after treatment with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h (N = 3). (E, F) HEK293 cells were cotransfected with EGFP and wild type TRPM8, mutant, or a control vector. After 48 h of transfection, EGFP-positive cells were selected for recording of TRPM8 channel-mediated current. The number of cells used for current recordings: Vector (6); WT: (10); V976W (12). (G, H) HeLa cells were transfected with the constructs for the expression of wild type or mutant TRPM8. After 48 h of transfection, cells were lysed and subjected to WB analysis using indicated antibodies (N = 3). N represents the number of replicate experiments. EGFP, enhanced green fluorescent protein; Ctrl, control; *P

    Techniques Used: Incubation, Western Blot, Mutagenesis, Plasmid Preparation, Transfection, Construct, Expressing

    Influence of AMPK impairment on the stimulatory effect of TRPM8 on autophagy. (A, B) MCF7 cells were transiently transfected with siRNA against human AMPK and a Flag-TRPM8 construct. After 48 h of transfection, protein lysates were extracted for WB analysis to determine the effect of TRPM8 overexpression on basal autophagy in the presence of AMPK knockdown (N = 3). (C, D) WB analysis of cell lysates of MDA-MB-231 cells treatment with 2 μM icilin, 10 μM menthol, or a combination of 10 M compound C for 48 h (N = 3). N represents the number of replicate experiments. *P
    Figure Legend Snippet: Influence of AMPK impairment on the stimulatory effect of TRPM8 on autophagy. (A, B) MCF7 cells were transiently transfected with siRNA against human AMPK and a Flag-TRPM8 construct. After 48 h of transfection, protein lysates were extracted for WB analysis to determine the effect of TRPM8 overexpression on basal autophagy in the presence of AMPK knockdown (N = 3). (C, D) WB analysis of cell lysates of MDA-MB-231 cells treatment with 2 μM icilin, 10 μM menthol, or a combination of 10 M compound C for 48 h (N = 3). N represents the number of replicate experiments. *P

    Techniques Used: Transfection, Construct, Western Blot, Over Expression, Multiple Displacement Amplification

    Involvement of the AMPK-ULK1-LC3 signaling cascade in TRPM8-stimulated autophagy. (A, B) The construct for Flag-AMPK expression was transiently transfected into MCF7 cells. After 48 h of transfection, the AMPK-ULK1-LC3 signaling cascade-related proteins were detected by WB (N = 3). (C, D) MCF7 cells were transiently transfected with wild type TRPM8, mutant V976W, or control vector. After 48 h of transfection, protein lysates were used for WB analysis (N = 3). (E, F) MDA-MB-231 cells treated with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h were extracted for WB analysis (N = 3). (G, H) MCF7 cells were transfected with siRNA against human TRPM8; siRNA against TRPM8 (siTRPM8-1 and siTRPM8-2) successfully knocked down TRPM8 expression compared with that in the control scramble siRNA samples according to WB analysis using an anti-TRPM8 antibody. The AMPK-ULK1-LC3 signaling cascade-related proteins detected by WB analysis using the indicated antibodies (N = 3). N represents the number of replicate experiments. *P
    Figure Legend Snippet: Involvement of the AMPK-ULK1-LC3 signaling cascade in TRPM8-stimulated autophagy. (A, B) The construct for Flag-AMPK expression was transiently transfected into MCF7 cells. After 48 h of transfection, the AMPK-ULK1-LC3 signaling cascade-related proteins were detected by WB (N = 3). (C, D) MCF7 cells were transiently transfected with wild type TRPM8, mutant V976W, or control vector. After 48 h of transfection, protein lysates were used for WB analysis (N = 3). (E, F) MDA-MB-231 cells treated with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h were extracted for WB analysis (N = 3). (G, H) MCF7 cells were transfected with siRNA against human TRPM8; siRNA against TRPM8 (siTRPM8-1 and siTRPM8-2) successfully knocked down TRPM8 expression compared with that in the control scramble siRNA samples according to WB analysis using an anti-TRPM8 antibody. The AMPK-ULK1-LC3 signaling cascade-related proteins detected by WB analysis using the indicated antibodies (N = 3). N represents the number of replicate experiments. *P

    Techniques Used: Construct, Expressing, Transfection, Western Blot, Mutagenesis, Plasmid Preparation, Multiple Displacement Amplification

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    Alomone Labs icilin
    Upregulation of autophagy by <t>TRPM8</t> in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM <t>icilin,</t> 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P
    Icilin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/icilin/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    icilin - by Bioz Stars, 2021-12
    93/100 stars
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    Upregulation of autophagy by TRPM8 in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM icilin, 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P

    Journal: Frontiers in Oncology

    Article Title: Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy

    doi: 10.3389/fonc.2020.573127

    Figure Lengend Snippet: Upregulation of autophagy by TRPM8 in various cancer cell lines. (A, B) The construct for TRPM8 expression was transiently transfected into MCF7 for 48 h; the cells were used for WB analysis using indicated antibodies to detect autophagy-associated proteins (N = 3). (C, D) Similar WB analysis of lysates of HCT116 cells transfected with TRPM8 (N = 3). (E–H) WB analysis of lysates of MDA-MB-231 cells treated with 10 μM menthol, 2 μM icilin, 0.5 μM AMTB, or combined treatments for 48 h (N = 3). N represents the number of replicate experiments. *P

    Article Snippet: To evaluate the effect of TRPM8 agonists or antagonist on cell autophagy, cells were treated for 48 h with TRPM8 agonists [10 µM menthol (Sangon Biotech, China) and 2 µM icilin (Alomone, Israel)] or a TRPM8 antagonist [0.5 µM AMTB hydrochloride (Alomone)].

    Techniques: Construct, Expressing, Transfection, Western Blot, Multiple Displacement Amplification

    Mechanism of the stimulatory effect of TRPM8 channel function on basal autophagy. (A, B) HeLa cells were incubated with menthol (10 μM) for 48 h. The cells were lysed and assayed by western blot using indicated antibodies (N = 3). (C, D) WB analysis of HeLa cell lysates after treatment with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h (N = 3). (E, F) HEK293 cells were cotransfected with EGFP and wild type TRPM8, mutant, or a control vector. After 48 h of transfection, EGFP-positive cells were selected for recording of TRPM8 channel-mediated current. The number of cells used for current recordings: Vector (6); WT: (10); V976W (12). (G, H) HeLa cells were transfected with the constructs for the expression of wild type or mutant TRPM8. After 48 h of transfection, cells were lysed and subjected to WB analysis using indicated antibodies (N = 3). N represents the number of replicate experiments. EGFP, enhanced green fluorescent protein; Ctrl, control; *P

    Journal: Frontiers in Oncology

    Article Title: Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy

    doi: 10.3389/fonc.2020.573127

    Figure Lengend Snippet: Mechanism of the stimulatory effect of TRPM8 channel function on basal autophagy. (A, B) HeLa cells were incubated with menthol (10 μM) for 48 h. The cells were lysed and assayed by western blot using indicated antibodies (N = 3). (C, D) WB analysis of HeLa cell lysates after treatment with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h (N = 3). (E, F) HEK293 cells were cotransfected with EGFP and wild type TRPM8, mutant, or a control vector. After 48 h of transfection, EGFP-positive cells were selected for recording of TRPM8 channel-mediated current. The number of cells used for current recordings: Vector (6); WT: (10); V976W (12). (G, H) HeLa cells were transfected with the constructs for the expression of wild type or mutant TRPM8. After 48 h of transfection, cells were lysed and subjected to WB analysis using indicated antibodies (N = 3). N represents the number of replicate experiments. EGFP, enhanced green fluorescent protein; Ctrl, control; *P

    Article Snippet: To evaluate the effect of TRPM8 agonists or antagonist on cell autophagy, cells were treated for 48 h with TRPM8 agonists [10 µM menthol (Sangon Biotech, China) and 2 µM icilin (Alomone, Israel)] or a TRPM8 antagonist [0.5 µM AMTB hydrochloride (Alomone)].

    Techniques: Incubation, Western Blot, Mutagenesis, Plasmid Preparation, Transfection, Construct, Expressing

    Influence of AMPK impairment on the stimulatory effect of TRPM8 on autophagy. (A, B) MCF7 cells were transiently transfected with siRNA against human AMPK and a Flag-TRPM8 construct. After 48 h of transfection, protein lysates were extracted for WB analysis to determine the effect of TRPM8 overexpression on basal autophagy in the presence of AMPK knockdown (N = 3). (C, D) WB analysis of cell lysates of MDA-MB-231 cells treatment with 2 μM icilin, 10 μM menthol, or a combination of 10 M compound C for 48 h (N = 3). N represents the number of replicate experiments. *P

    Journal: Frontiers in Oncology

    Article Title: Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy

    doi: 10.3389/fonc.2020.573127

    Figure Lengend Snippet: Influence of AMPK impairment on the stimulatory effect of TRPM8 on autophagy. (A, B) MCF7 cells were transiently transfected with siRNA against human AMPK and a Flag-TRPM8 construct. After 48 h of transfection, protein lysates were extracted for WB analysis to determine the effect of TRPM8 overexpression on basal autophagy in the presence of AMPK knockdown (N = 3). (C, D) WB analysis of cell lysates of MDA-MB-231 cells treatment with 2 μM icilin, 10 μM menthol, or a combination of 10 M compound C for 48 h (N = 3). N represents the number of replicate experiments. *P

    Article Snippet: To evaluate the effect of TRPM8 agonists or antagonist on cell autophagy, cells were treated for 48 h with TRPM8 agonists [10 µM menthol (Sangon Biotech, China) and 2 µM icilin (Alomone, Israel)] or a TRPM8 antagonist [0.5 µM AMTB hydrochloride (Alomone)].

    Techniques: Transfection, Construct, Western Blot, Over Expression, Multiple Displacement Amplification

    Involvement of the AMPK-ULK1-LC3 signaling cascade in TRPM8-stimulated autophagy. (A, B) The construct for Flag-AMPK expression was transiently transfected into MCF7 cells. After 48 h of transfection, the AMPK-ULK1-LC3 signaling cascade-related proteins were detected by WB (N = 3). (C, D) MCF7 cells were transiently transfected with wild type TRPM8, mutant V976W, or control vector. After 48 h of transfection, protein lysates were used for WB analysis (N = 3). (E, F) MDA-MB-231 cells treated with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h were extracted for WB analysis (N = 3). (G, H) MCF7 cells were transfected with siRNA against human TRPM8; siRNA against TRPM8 (siTRPM8-1 and siTRPM8-2) successfully knocked down TRPM8 expression compared with that in the control scramble siRNA samples according to WB analysis using an anti-TRPM8 antibody. The AMPK-ULK1-LC3 signaling cascade-related proteins detected by WB analysis using the indicated antibodies (N = 3). N represents the number of replicate experiments. *P

    Journal: Frontiers in Oncology

    Article Title: Transient Receptor Potential Melastatin 8 (TRPM8) Channel Regulates Proliferation and Migration of Breast Cancer Cells by Activating the AMPK-ULK1 Pathway to Enhance Basal Autophagy

    doi: 10.3389/fonc.2020.573127

    Figure Lengend Snippet: Involvement of the AMPK-ULK1-LC3 signaling cascade in TRPM8-stimulated autophagy. (A, B) The construct for Flag-AMPK expression was transiently transfected into MCF7 cells. After 48 h of transfection, the AMPK-ULK1-LC3 signaling cascade-related proteins were detected by WB (N = 3). (C, D) MCF7 cells were transiently transfected with wild type TRPM8, mutant V976W, or control vector. After 48 h of transfection, protein lysates were used for WB analysis (N = 3). (E, F) MDA-MB-231 cells treated with 2 μM icilin, 0.5 μM AMTB, or their combination for 48 h were extracted for WB analysis (N = 3). (G, H) MCF7 cells were transfected with siRNA against human TRPM8; siRNA against TRPM8 (siTRPM8-1 and siTRPM8-2) successfully knocked down TRPM8 expression compared with that in the control scramble siRNA samples according to WB analysis using an anti-TRPM8 antibody. The AMPK-ULK1-LC3 signaling cascade-related proteins detected by WB analysis using the indicated antibodies (N = 3). N represents the number of replicate experiments. *P

    Article Snippet: To evaluate the effect of TRPM8 agonists or antagonist on cell autophagy, cells were treated for 48 h with TRPM8 agonists [10 µM menthol (Sangon Biotech, China) and 2 µM icilin (Alomone, Israel)] or a TRPM8 antagonist [0.5 µM AMTB hydrochloride (Alomone)].

    Techniques: Construct, Expressing, Transfection, Western Blot, Mutagenesis, Plasmid Preparation, Multiple Displacement Amplification