trpv1  (Alomone Labs)


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    Name:
    Capsaicin
    Description:
    Capsaicin is one of the most powerful chemical agonists of TRPV1 Transient Receptor Potential Vanilloid channel It is a small lipophilic molecule derived from hot chili peppers that acts on sensory neurons by opening the TRPV1 a principal transduction channel for nociception Capsaicin acts directly on TRPV1 with half maximal effective concentration of 711 9 nM in xenopus oocytes and 0 29 muM in HEK293 cells
    Catalog Number:
    C-125
    Price:
    45.0
    Category:
    Small Molecule
    Source:
    Synthetic
    Applications:
    0
    Purity:
    >98% (HPLC)
    Size:
    50 mg
    Format:
    Lyophilized/solid.
    Formula:
    C18H27NO3
    Molecular Weight:
    305.4
    Molecule Name:
    (E)-N-[(4-Hydroxy-3-methoxyphenyl)methyl]-8-methyl-6-nonenamide.
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    Structured Review

    Alomone Labs trpv1
    Capsaicin
    Capsaicin is one of the most powerful chemical agonists of TRPV1 Transient Receptor Potential Vanilloid channel It is a small lipophilic molecule derived from hot chili peppers that acts on sensory neurons by opening the TRPV1 a principal transduction channel for nociception Capsaicin acts directly on TRPV1 with half maximal effective concentration of 711 9 nM in xenopus oocytes and 0 29 muM in HEK293 cells
    https://www.bioz.com/result/trpv1/product/Alomone Labs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpv1 - by Bioz Stars, 2021-09
    97/100 stars

    Images

    1) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

    2) Product Images from "Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice"

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00451-0

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    TRPV1 and related molecular pathways in the mouse brain
    Figure Legend Snippet: TRPV1 and related molecular pathways in the mouse brain

    Techniques Used:

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups
    Figure Legend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Techniques Used: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups
    Figure Legend Snippet: Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Techniques Used: Mouse Assay

    Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups
    Figure Legend Snippet: Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Techniques Used: Mouse Assay, Western Blot, Expressing

    3) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    4) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    5) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    6) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    7) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    8) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    9) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    10) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    11) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    12) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    13) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    14) Product Images from "Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice"

    Article Title: Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000264

    NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P
    Figure Legend Snippet: NVI increased TRPA1 protein expression in the vagina but not in primary sensory neurons innervating the vagina. (A) Representative Western blots are shown for TRPA1 and corresponding GAPDH protein expression with bands at 127 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPA1 protein expression was significantly increased in vagina, but not DRG, from NVI mice compared with naive mice. (B) Representative Western blots are shown for TRPV1 and corresponding GAPDH protein expression with bands at 85 and 35 kD, respectively, in both DRG and vagina from naive and NVI mice. TRPV1 protein expression was not significantly different in NVI DRG or vagina compared with naive counterparts. Calcium imaging was performed on lumbosacral (L5-S1) DRG neurons retrogradely labeled by injection of Alexa Fluor–conjugated cholera toxin-β (CTB) into the distal vagina, 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) into the perivaginal skin, and adjacent unlabeled DRG to measure responses to 100 μM mustard oil (MO; C) and 1 μM capsaicin (D). (C) No significant difference in functional TRPA1 expression, measured as the percentage of MO-responsive DRG neurons, was observed between naive and NVI mice for any population of DRG neurons tested. (D) Only in naive mice, DRG neurons back-labeled from the vagina were significantly more likely to respond to 1 μM capsaicin, suggesting greater functional TRPV1 expression, compared with those back-labeled from the perivaginal skin or unlabeled DRG. When compared across both agonists, vagina-specific DRG neurons from NVI mice had a significantly reduced percentage of responsive neurons compared with naive ( P

    Techniques Used: Expressing, Western Blot, Mouse Assay, Imaging, Labeling, Injection, CtB Assay, Functional Assay

    15) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    16) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    17) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    18) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    19) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    20) Product Images from "Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture"

    Article Title: Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2012/636848

    Electrophysiological properties of AP (action potential) and TRPV1-mediated inward currents in the Con (control), CFA (complete Freund's adjuvant), and EA (electroacupuncture) groups. (a) The AP threshold was more negative in the CFA group than in the control group. EA attenuated neuron excitability by reversing the AP threshold to a positive membrane potential. (b) CFA-induced inflammation decreased both the rising and falling times compared with the control group. EA treatment decreased the neuron excitability to the control level. (c) The capsaicin- (cap-) induced inward current was increased in CFA-treated DRG neurons compared with the control group. This was reversed by EA manipulation.
    Figure Legend Snippet: Electrophysiological properties of AP (action potential) and TRPV1-mediated inward currents in the Con (control), CFA (complete Freund's adjuvant), and EA (electroacupuncture) groups. (a) The AP threshold was more negative in the CFA group than in the control group. EA attenuated neuron excitability by reversing the AP threshold to a positive membrane potential. (b) CFA-induced inflammation decreased both the rising and falling times compared with the control group. EA treatment decreased the neuron excitability to the control level. (c) The capsaicin- (cap-) induced inward current was increased in CFA-treated DRG neurons compared with the control group. This was reversed by EA manipulation.

    Techniques Used:

    TRPV1 and TRPV4 expression was increased in DRG (dorsal root ganglia) after intraplantar carrageenan injection and attenuated by electroacupuncture (EA) at the ST36 acupoint in mice. (a–c) Mouse DRGs were stained with TRPV1 antibody. (d–e) Mouse DRGs were stained with TRPV4 antibody. Arrows indicate positive antibody reactions. Scale bar = 50 μ m for all panels. Con: control; Carra: carrageenan-induced; EA: electroacupuncture at ST36.
    Figure Legend Snippet: TRPV1 and TRPV4 expression was increased in DRG (dorsal root ganglia) after intraplantar carrageenan injection and attenuated by electroacupuncture (EA) at the ST36 acupoint in mice. (a–c) Mouse DRGs were stained with TRPV1 antibody. (d–e) Mouse DRGs were stained with TRPV4 antibody. Arrows indicate positive antibody reactions. Scale bar = 50 μ m for all panels. Con: control; Carra: carrageenan-induced; EA: electroacupuncture at ST36.

    Techniques Used: Expressing, Injection, Mouse Assay, Staining

    Cell area percentage of TRPV1-immunoreactive neurons in the L3-L5 DRG in mice in control, carrageenan, carrageenan with EA (electroacupuncture), CFA (complete Freund's adjuvant), and CFA with EA (electroacupuncture) treatment. (a) The percentage of TRPV1-positive neurons from lumbar DRGs that belonged to corresponding cell area was presented. At day 4, TRPV1-positive neurons were dominantly observed in small neurons (cell area
    Figure Legend Snippet: Cell area percentage of TRPV1-immunoreactive neurons in the L3-L5 DRG in mice in control, carrageenan, carrageenan with EA (electroacupuncture), CFA (complete Freund's adjuvant), and CFA with EA (electroacupuncture) treatment. (a) The percentage of TRPV1-positive neurons from lumbar DRGs that belonged to corresponding cell area was presented. At day 4, TRPV1-positive neurons were dominantly observed in small neurons (cell area

    Techniques Used: Mouse Assay

    TRPV1 and TRPV4 protein levels. DRG (dorsal root ganglion) lysates underwent immunoreactions with specific TRPV1 (a and b) and TRPV4 (c and d) antibodies. TRPV1 and TRPV4 increased substantially after carra (carrageenan) or CFA (complete Freund's adjuvant) injection as compared with the saline-injected group (Con). TRPV1 and TRPV4 protein levels were attenuated by electroacupuncture (EA) at the ST36 acupoint as compared with the carra- and CFA-induced groups.
    Figure Legend Snippet: TRPV1 and TRPV4 protein levels. DRG (dorsal root ganglion) lysates underwent immunoreactions with specific TRPV1 (a and b) and TRPV4 (c and d) antibodies. TRPV1 and TRPV4 increased substantially after carra (carrageenan) or CFA (complete Freund's adjuvant) injection as compared with the saline-injected group (Con). TRPV1 and TRPV4 protein levels were attenuated by electroacupuncture (EA) at the ST36 acupoint as compared with the carra- and CFA-induced groups.

    Techniques Used: Injection

    TRPV1 and TRPV4 expression was increased in DRG (dorsal root ganglia) after intraplantar CFA (complete Freund's adjuvant) injection and attenuated by EA (electroacupuncture) at the ST36 acupoint in mice. (a–c) Mouse DRGs were stained with TRPV1 antibody. (d–e) Mouse DRGs were stained with TRPV4 antibody. Arrows indicate positive antibody reactions. Scale bar = 50 μ m for all panels. Con: control. CFA: CFA-induced; EA: electroacupuncture at ST36.
    Figure Legend Snippet: TRPV1 and TRPV4 expression was increased in DRG (dorsal root ganglia) after intraplantar CFA (complete Freund's adjuvant) injection and attenuated by EA (electroacupuncture) at the ST36 acupoint in mice. (a–c) Mouse DRGs were stained with TRPV1 antibody. (d–e) Mouse DRGs were stained with TRPV4 antibody. Arrows indicate positive antibody reactions. Scale bar = 50 μ m for all panels. Con: control. CFA: CFA-induced; EA: electroacupuncture at ST36.

    Techniques Used: Expressing, Injection, Mouse Assay, Staining

    21) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

    22) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    23) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    24) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    25) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    26) Product Images from "Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine"

    Article Title: Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00356.2012

    Intraluminal transient receptor potential (TRP) vanilloid type 1 (TRPV1) and TRP ankyrin 1 (TRPA1) agonists increase blood flow in the small intestine. Capsaicin (CAP, 3 mg/kg body wt) or allyl isothiocyanate (AITC, 0.002 mg/kg body wt) was administered
    Figure Legend Snippet: Intraluminal transient receptor potential (TRP) vanilloid type 1 (TRPV1) and TRP ankyrin 1 (TRPA1) agonists increase blood flow in the small intestine. Capsaicin (CAP, 3 mg/kg body wt) or allyl isothiocyanate (AITC, 0.002 mg/kg body wt) was administered

    Techniques Used: Flow Cytometry

    TRPA1 and ADM expression in intestinal epithelial cells. A : RT-PCR analysis was performed for TRPA1, TRPV1, ADM, and β-actin in rat intestinal epithelial (IE) cells of the small intestine (S-IE), those of the large intestine (L-IE), dorsal root
    Figure Legend Snippet: TRPA1 and ADM expression in intestinal epithelial cells. A : RT-PCR analysis was performed for TRPA1, TRPV1, ADM, and β-actin in rat intestinal epithelial (IE) cells of the small intestine (S-IE), those of the large intestine (L-IE), dorsal root

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    27) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    28) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    29) Product Images from "TRPV1 Responses in the Cerebellum Lobules VI, VII, VIII Using Electroacupuncture Treatment for Chronic Pain and Depression Comorbidity in a Murine Model"

    Article Title: TRPV1 Responses in the Cerebellum Lobules VI, VII, VIII Using Electroacupuncture Treatment for Chronic Pain and Depression Comorbidity in a Murine Model

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22095028

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. ( A ) The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) Conversely, no significant variance in pNFkB density was observed in cerebellum lobule VIb among the 5 groups, although TRPV1 maintained an analogous trend of decreased expression in the AS and AS + SHAM group, which was absent in the AS + KO group and increased in the AS + EA group when compared to Control. Scale bar is 50 μm.
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VIa and VIb. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. ( A ) The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobule VIa. ( B ) Conversely, no significant variance in pNFkB density was observed in cerebellum lobule VIb among the 5 groups, although TRPV1 maintained an analogous trend of decreased expression in the AS and AS + SHAM group, which was absent in the AS + KO group and increased in the AS + EA group when compared to Control. Scale bar is 50 μm.

    Techniques Used: Immunofluorescence, Staining, Expressing

    Illustration of CPDC pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz/20 min, and TRPV1 gene deletion can increase the expressions of TRPV1, Nav1.7, Nav1.8, GABAAα1, NMDAR1, and TrkB receptors in the AS-induced CPDC model, as observed in the AS + EA and AS + KO groups. Furthermore, the relevant increases in related responses cause effective increases in the signaling of pPI3K, pAkt, pERK, pmTOR, pPKCε, pPKAIIα, pNFκB, and pCREB under conditions of CPDC, and these serve to present a basic neuromodulatory pathway of CPDC, TRPV1 signaling, and the molecular function of EA.
    Figure Legend Snippet: Illustration of CPDC pathways attenuated by EA in the cerebellum. We found that EA at 1 mA, 2 Hz/20 min, and TRPV1 gene deletion can increase the expressions of TRPV1, Nav1.7, Nav1.8, GABAAα1, NMDAR1, and TrkB receptors in the AS-induced CPDC model, as observed in the AS + EA and AS + KO groups. Furthermore, the relevant increases in related responses cause effective increases in the signaling of pPI3K, pAkt, pERK, pmTOR, pPKCε, pPKAIIα, pNFκB, and pCREB under conditions of CPDC, and these serve to present a basic neuromodulatory pathway of CPDC, TRPV1 signaling, and the molecular function of EA.

    Techniques Used:

    The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VIII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly augmented in the AS + EA and AS + KO groups. Both the AS + EA and AS + KO groups displayed no difference when compared to the Control group, depicting an observable improvement of CPDC tendencies. However, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of significant increases in the AS and AS + SHAM groups when compared to the Control group. Furthermore, this increase was significantly ameliorated in the AS + EA and AS + KO groups, which retained states of non-significance when similarly compared to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VIII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly augmented in the AS + EA and AS + KO groups. Both the AS + EA and AS + KO groups displayed no difference when compared to the Control group, depicting an observable improvement of CPDC tendencies. However, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of significant increases in the AS and AS + SHAM groups when compared to the Control group. Furthermore, this increase was significantly ameliorated in the AS + EA and AS + KO groups, which retained states of non-significance when similarly compared to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB and ( J ) pNFκB ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Interestingly, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of no significant variances across all 5 groups. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in the cerebellum lobule VII. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB and ( J ) pNFκB ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Interestingly, the protein expression levels of ( C ) Nav1.7 and ( D ) Nav1.8 displayed states of no significant variances across all 5 groups. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    The expression levels of nociceptive receptors and associated molecules in cerebellum lobule VI. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Conversely, the protein expressions of the AS and AS + SHAM groups were significantly increased in ( C ) Nav1.7 and ( D ) Nav1.8 when compared to the Control group. Correspondingly, the AS + EA and AS + KO groups were augmented and showed no significant difference in comparison to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p
    Figure Legend Snippet: The expression levels of nociceptive receptors and associated molecules in cerebellum lobule VI. The immunoblotting images depict five lanes of protein in the following order: Control, AS, AS + EA, AS + SHAM, and AS + KO groups. There are significant decreases in protein expression in the AS and AS + SHAM groups of ( A ) TRPV1, ( B ) pmTOR, ( E ) pPI3K, ( F ) NMDAR1, ( G ) pPKCε, ( H ) pAkt, ( I ) TrkB, ( J ) pNFκB, ( K ) GABAAα1, ( L ) pPKAIIα, ( M ) pCREB, and ( N ) pERK levels, which were significantly attenuated in the AS + EA and AS + KO groups, depicting no difference when compared to the Control group. Conversely, the protein expressions of the AS and AS + SHAM groups were significantly increased in ( C ) Nav1.7 and ( D ) Nav1.8 when compared to the Control group. Correspondingly, the AS + EA and AS + KO groups were augmented and showed no significant difference in comparison to the Control group. Accordingly, the protein density of the AS + KO group revealed a predicted decrease in ( A ) TRPV1 (* p

    Techniques Used: Expressing

    Comparative graph of paw withdrawal threshold and latency of the Acid-Saline (AS)-induced chronic pain subjects after electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion (KO). Control, AS, AS + EA, AS + SHAM, and AS + KO were tested according to ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ nociceptive sensitivities. * p
    Figure Legend Snippet: Comparative graph of paw withdrawal threshold and latency of the Acid-Saline (AS)-induced chronic pain subjects after electroacupuncture (EA) treatment and transient receptor vanilloid member 1 (TRPV1) gene deletion (KO). Control, AS, AS + EA, AS + SHAM, and AS + KO were tested according to ( A ) mechanical von Frey and ( B ) thermal Hargreaves’ nociceptive sensitivities. * p

    Techniques Used:

    Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VII and VIII. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobules ( A ) VII and ( B ) VIII. Scale bar is 50 μm.
    Figure Legend Snippet: Immunofluorescence staining of TRPV1 and pNFkB protein expression in the cerebellum lobules VII and VIII. There are 5 subject groups: Control, AS, AS + EA, AS + SHAM, and AS + KO. The efficacy of EA treatment involves significant increases in TRPV1 and pNFkB densities in the cerebellum lobules ( A ) VII and ( B ) VIII. Scale bar is 50 μm.

    Techniques Used: Immunofluorescence, Staining, Expressing

    30) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    31) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    32) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    33) Product Images from "TRPV1 neurons regulate β-cell function in a sex-dependent manner"

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2018.10.002

    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Figure Legend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Techniques Used: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).
    Figure Legend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Techniques Used: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).
    Figure Legend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Techniques Used: Mouse Assay, Injection, Activity Assay

    34) Product Images from "Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)"

    Article Title: Differential expression and localization of thermosensitive Transient Receptor Potential Vanilloid (TRPV) channels in the mature sperm of white pekin duck (Anas platyrhynchos)

    Journal: bioRxiv

    doi: 10.1101/2020.02.10.941732

    Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P
    Figure Legend Snippet: Prevalence of physiologically relevant thermosensitive TRPV channels in mature duck sperm. Flow cytometric evaluation of duck sperm stained for physiologically relevant thermosensitive TRPV channels are shown. A. Representative dot-plots showing percentage of cells expressing TRPV1, TRPV3, TRPV4 channels detected by Ab-1 (antibodies from Alomone labs) antibody specific for each TRPV channel. B. Histograms showing percentage of cells expressing TRPV channels and corresponding Mean Fluorescence Intensity (MFI) of TRPV channels detected by Ab2 antibody of each channel (from Sigma Aldrich), expressed as fold change in comparison to MFI of unstained cells. n = 3, unpaired T-test. ** = P

    Techniques Used: Staining, Expressing, Fluorescence

    Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.
    Figure Legend Snippet: Endogenous expression of TRPV channels in duck sperm. Western blot analysis of duck sperm extracts probed with different TRPV-specific antibodies are shown. A. TRPV1 specific band is detected by a specific antibody (directed against the C-terminus of TRPV1, Alomone Labs) in absence but not in presence of its blocking peptide; B. Western blot analysis with antibody that detects TRPV2 (raised against the C-terminus, Alomone Labs). C. Two different antibodies detecting TRPV3 [raised against the C-terminus, (Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect similar expression pattern of TRPV3. D. Two different antibodies raised against the TRPV4 [raised against C-terminus, Ab1: Alomone Labs) and N-terminus (Ab3: Sigma Aldrich)] detect TRPV4 at the expected size. E. Two different antibodies raised against the C-terminus of TRPV5 (Ab1: Alomone Labs and Ab2: Sigma-Aldrich) detects TRPV5 at expected size. F. A specific antibody raised against the C-terminus of TRPV6 (Ab-1: Alomone Labs) detects TRPV6 in absence but not in presence of its blocking peptide.

    Techniques Used: Expressing, Western Blot, Blocking Assay

    Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.
    Figure Legend Snippet: Microscopic images showing localization of TRPV1 in duck sperm. A-B. Confocal microscopic images depicting the localization of TRPV1 (green) as detected by two different antibodies and Nucleus (blue) by DAPI. Mitochondria (red) is labelled by Mitotracker Red dye in A and C. White arrows indicate the mitochondrial region where expression of TRPV1 is typically absent. C . SR-SIM images of TRPV1 localization (using Ab1 antibody) at the head (left) and tail (right) of duck sperm is shown. D . Zoomed up image of neck region of sperm depicting the absence of TRPV1 (green) in the neck region. The head (blue) and arrows marking the start and end point of mitochondrial region.

    Techniques Used: Expressing

    35) Product Images from "Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway"

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00468-5

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p
    Figure Legend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Techniques Used: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p
    Figure Legend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Techniques Used: Expressing, Western Blot

    36) Product Images from "Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model"

    Article Title: Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4462

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the NTS. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the NTS of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein; NTS, nucleus tractus solitarii.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the hypothalamus. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results revealed significant increases in (A) TRPV1, (B) p-PI3K, (C) p-Akt, (D) p-mTOR, (E) p-PKCε, (F) p-PKAIIα, (G) p-ERK, (H) p-p38, (I) p-JNK, (J) p-NF-κB and (K) p-CREB expression levels in the WT-HFD and WT-HFD-SHAM groups compared with the other groups ( * P

    Techniques Used: Expressing

    Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P
    Figure Legend Snippet: Expression levels of TRPV1 and associated molecules in the PFC. The expression pattern of TRPV1 protein was detected in the following groups: WT-HFD; WT-HFD-ACE; WT-HFD-SHAM; and KO-HFD. The results demonstrated significant decreases in (A) TRPV1 expression in WT-HFD, WT-HFD-SHAM and KO-HFD groups when compared with the WT-HFD-ACE group, which demonstrated a significant increase following ACE treatment. * P

    Techniques Used: Expressing

    Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.
    Figure Legend Snippet: Expression levels of TRPV1, p-PI3K, p-CREB and p-PKAIIα in the hypothalamus. (A) Representative immunofluorescence staining of TRPV1 (green) and p-PKAIIα (red) and (B) representative immunofluorescence staining of p-PI3K (red) and p-CREB (green) were performed in the hypothalamus of subjects in the WT-HFD, WT-HFD-ACE, WT-HFD-SHAM and KO-HFD groups. White arrowheads indicate immunopositive cells. TRPV1, transient receptor vanilloid member 1; WT, wild-type; ND, normal diet; HFD, high-fat diet; ACE, acupoint catgut embedding; KO, knockout; p, phosphorylated; PKAIIα, protein kinase AII α; CREB, cyclic AMP-response element binding protein.

    Techniques Used: Expressing, Immunofluorescence, Staining, Knock-Out, Binding Assay

    Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P
    Figure Legend Snippet: Weekly body weight alterations and food consumption in the six subject groups. (A) The graph presents comparisons of body weight in the WT-ND, WT-HFD, WT-HFD-ACE, WT-HFD-SHAM, KO-ND and KO-HFD groups. Significant body weight increases in the WT-HFD, WT-HFD-ACE and WT-HFD-SHAM groups compared to the WT-ND group and both TRPV1 KO mouse groups were observed. * P

    Techniques Used:

    37) Product Images from "Silencing of spinal Trpv1 attenuates neuropathic pain in rats by inhibiting CAMKII expression and ERK2 phosphorylation"

    Article Title: Silencing of spinal Trpv1 attenuates neuropathic pain in rats by inhibiting CAMKII expression and ERK2 phosphorylation

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-39184-4

    The intrathecal injection of the TRPV1 siRNA in vivo attenuated the mechanical and thermal hyperalgesia induced by chronic constriction injury. ( A ) The administration of the TRPV1 siRNA (5 μg/15 μl) once daily for two days significantly increased the paw withdrawal threshold to mechanical stimuli on days 1 to 4 post-transfection compared to the CCI + PEI control group or to the pre-injection baseline. ( B ) The TRPV1 siRNA significantly increased the paw withdrawal latency in response to thermal stimuli on days 1 to 4 post-transfection compared to results in the CCI + PEI control group or the pre-injection baseline. ** P
    Figure Legend Snippet: The intrathecal injection of the TRPV1 siRNA in vivo attenuated the mechanical and thermal hyperalgesia induced by chronic constriction injury. ( A ) The administration of the TRPV1 siRNA (5 μg/15 μl) once daily for two days significantly increased the paw withdrawal threshold to mechanical stimuli on days 1 to 4 post-transfection compared to the CCI + PEI control group or to the pre-injection baseline. ( B ) The TRPV1 siRNA significantly increased the paw withdrawal latency in response to thermal stimuli on days 1 to 4 post-transfection compared to results in the CCI + PEI control group or the pre-injection baseline. ** P

    Techniques Used: Injection, In Vivo, Transfection

    Reduced TRPV1 and CAMKII expression, as well as decreased ERK phosphorylation, in the spinal dorsal horn following TRPV1 siRNA administration. Western blots showing the reduced expression of the TRPV1 protein in the spinal dorsal horn 24 hours after the intrathecal injection of the TRPV1 siRNA (5 μg/15 μl) once daily for 2 days, in both naive (A) and CCI (C) rats. (B , D) Bar chart depicting the level of TRPV1 protein relative to that in the group administered PEI alone; the level of TRPV1 in the spinal dorsal horn was significantly reduced. (E) Western blots showing the reduced expression of CAMKII following the intrathecal injection of the TRPV1 siRNA compared to the results in the CCI + PEI group. (F) Bar chart presenting the protein content relative to the CCI + PEI group; the CAMKII level was significantly reduced. (G) Western blots showing the reduced level of phosphorylated ERK2 following the intrathecal injection of the TRPV1 siRNA in comparison to the results in the CCI + PEI group. (H) Bar chart depicting the protein content relative to the CCI + PEI group; the level of phosphorylated ERK2 was significantly reduced in the CCI + siRNA group. Tubulin or GAPDH served as the loading control and was run on the same blot. TRPV1 levels were significantly reduced in the siRNA group compared with those in the PEI group in naive rats. ** P = 0.001 compared with those in the PEI group (ANOVA, n = 5 rats per group). TRPV1 levels were significantly reduced in the CCI + siRNA group compared with those in the CCI + PEI group in CCI rats. ** P = 0.003 compared with those in the CCI + PEI group ( t -test, n = 4 rats per group). The level of CAMKII in the ipsilateral spinal dorsal horn of the CCI + siRNA group was significantly lower than that in the CCI + PEI group. * P = 0.026 compared with that in the CCI + PEI group ( t -test, n = 3 rats per group). The level of phosphorylated ERK2 in the ipsilateral spinal dorsal horn of the CCI + RNAi group was significantly lower than that of the CCI + PEI group. ** P = 0.002 compared with that in the CCI + PEI group (Mann-Whitney U test, n = 6 rats per group). Error bars represent the SEM. PEI: polyethylenimine; CCI: chronic constriction injury.
    Figure Legend Snippet: Reduced TRPV1 and CAMKII expression, as well as decreased ERK phosphorylation, in the spinal dorsal horn following TRPV1 siRNA administration. Western blots showing the reduced expression of the TRPV1 protein in the spinal dorsal horn 24 hours after the intrathecal injection of the TRPV1 siRNA (5 μg/15 μl) once daily for 2 days, in both naive (A) and CCI (C) rats. (B , D) Bar chart depicting the level of TRPV1 protein relative to that in the group administered PEI alone; the level of TRPV1 in the spinal dorsal horn was significantly reduced. (E) Western blots showing the reduced expression of CAMKII following the intrathecal injection of the TRPV1 siRNA compared to the results in the CCI + PEI group. (F) Bar chart presenting the protein content relative to the CCI + PEI group; the CAMKII level was significantly reduced. (G) Western blots showing the reduced level of phosphorylated ERK2 following the intrathecal injection of the TRPV1 siRNA in comparison to the results in the CCI + PEI group. (H) Bar chart depicting the protein content relative to the CCI + PEI group; the level of phosphorylated ERK2 was significantly reduced in the CCI + siRNA group. Tubulin or GAPDH served as the loading control and was run on the same blot. TRPV1 levels were significantly reduced in the siRNA group compared with those in the PEI group in naive rats. ** P = 0.001 compared with those in the PEI group (ANOVA, n = 5 rats per group). TRPV1 levels were significantly reduced in the CCI + siRNA group compared with those in the CCI + PEI group in CCI rats. ** P = 0.003 compared with those in the CCI + PEI group ( t -test, n = 4 rats per group). The level of CAMKII in the ipsilateral spinal dorsal horn of the CCI + siRNA group was significantly lower than that in the CCI + PEI group. * P = 0.026 compared with that in the CCI + PEI group ( t -test, n = 3 rats per group). The level of phosphorylated ERK2 in the ipsilateral spinal dorsal horn of the CCI + RNAi group was significantly lower than that of the CCI + PEI group. ** P = 0.002 compared with that in the CCI + PEI group (Mann-Whitney U test, n = 6 rats per group). Error bars represent the SEM. PEI: polyethylenimine; CCI: chronic constriction injury.

    Techniques Used: Expressing, Western Blot, Injection, MANN-WHITNEY

    Increased expression levels of TRPV1, CAMKII, and phosphorylated ERK in the ipsilateral dorsal horn induced by chronic constriction injury. ( A ) The loose ligation of the sciatic nerve significantly increased TRPV1 and CAMKII expression in the ipsilateral spinal dorsal horn 7 days after CCI. ( B ) Bar chart showing the protein content relative to the sham group. The levels of TRPV1 and CAMKII in the ipsilateral spinal dorsal horn of the CCI group were significantly higher than those in the sham group ( P = 0.031 for TRPV1; P = 0.018 for CAMKII). ( C ) Loose ligation of the sciatic nerve significantly increased ERK phosphorylation in the ipsilateral spinal dorsal horn. ( D , E ) Bar charts depicting the protein content relative to that in the sham group. A significantly higher level of phosphorylated ERK2 was observed in the ipsilateral spinal dorsal horn of the CCI group than in the ipsilateral spinal dorsal horn of the sham group ( P = 0.023). GAPDH or tubulin served as a loading control and was run on the same blot. * P
    Figure Legend Snippet: Increased expression levels of TRPV1, CAMKII, and phosphorylated ERK in the ipsilateral dorsal horn induced by chronic constriction injury. ( A ) The loose ligation of the sciatic nerve significantly increased TRPV1 and CAMKII expression in the ipsilateral spinal dorsal horn 7 days after CCI. ( B ) Bar chart showing the protein content relative to the sham group. The levels of TRPV1 and CAMKII in the ipsilateral spinal dorsal horn of the CCI group were significantly higher than those in the sham group ( P = 0.031 for TRPV1; P = 0.018 for CAMKII). ( C ) Loose ligation of the sciatic nerve significantly increased ERK phosphorylation in the ipsilateral spinal dorsal horn. ( D , E ) Bar charts depicting the protein content relative to that in the sham group. A significantly higher level of phosphorylated ERK2 was observed in the ipsilateral spinal dorsal horn of the CCI group than in the ipsilateral spinal dorsal horn of the sham group ( P = 0.023). GAPDH or tubulin served as a loading control and was run on the same blot. * P

    Techniques Used: Expressing, Ligation

    Related Articles

    Incubation:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. PVDF membrane was blocked in 5% skimmed milk in TBST buffer (Tris–HCl, 5 mM, pH 7.6, NaCl 136 mM, 0.05% Tween-20) at room temperature for 2 h, followed by incubation with different primary antibodies—β-actin (1:5000, Bioworld, USA), p38 (1:1000, Abcam, USA), p-p38 (1:1000, Cell Signaling Technology, USA), PKCε (1:1000, Abcam, USA) and TRPV1 (1:200, Alomone, Israel) in TBST at 4 ℃ overnight. ..

    other:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: Recent studies have shown that one of the targets of PKCε in peripheral nociceptive signaling is TRPV1 [ , – ].

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: Thus, PKCε was an upstream molecule of p38 and TRPV1 in PIPN cell models to improve the inflammation state of DRG neurons.

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: Although the inhibitory effect of PKCε inhibitors on TRPV1 and PKCε protein is stronger than the inhibitory effect of CSBTA, 50 μg/ml CSBTA has a more obvious downregulation of p-p38 MAPK, suggesting that CSBTA has multiple targets.

    Injection:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Hara et al. [ ] have confirmed that intraperitoneal injection of paclitaxel could increase TRPV1 in rat plantar skin and sensitize the TRPV1 channel, which is regarded as the cause of heat hyperalgesia. ..

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Inflammatory states or injection of some cytokines (such as bradykinin and nerve growth factor) could develop into severe thermal hyperalgesia, and TRPV1−/− mice would provide genetic support for the idea that TRPV1 is a crucial component of inflammation-induced hyperalgesia [ ]. ..

    Blocking Assay:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Figure f showed that blocking PKCε or p38 MAPK could significantly inhibit the mRNA level of TRPV1. ..

    Mouse Assay:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Inflammatory states or injection of some cytokines (such as bradykinin and nerve growth factor) could develop into severe thermal hyperalgesia, and TRPV1−/− mice would provide genetic support for the idea that TRPV1 is a crucial component of inflammation-induced hyperalgesia [ ]. ..

    Inhibition:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Inhibition of p-p38 MAPK expression can significantly reduce the content of TRPV1 in the neuronal body and skin tissue and alleviate the temperature sensitivity caused by inflammation [ , ]. ..

    Expressing:

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway
    Article Snippet: .. Inhibition of p-p38 MAPK expression can significantly reduce the content of TRPV1 in the neuronal body and skin tissue and alleviate the temperature sensitivity caused by inflammation [ , ]. ..

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    Alomone Labs trpv1
    Chemodenervation of pancreas-projecting <t>TRPV1</t> afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).
    Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Journal: Molecular Metabolism

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    doi: 10.1016/j.molmet.2018.10.002

    Figure Lengend Snippet: Chemodenervation of pancreas-projecting TRPV1 afferents enhances β-cell function and glucose tolerance in a sex-dependent manner: Five-week-old male and female C57BL/6J mice received a pancreatic intraductal injection of 50 μg of capsaicin per 100 μl of vehicle or vehicle alone. Ten days post-treatment, metabolic phenotyping was performed. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Glucose-stimulated insulin secretion (males). E. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from male mice injected with vehicle (upper panel) or capsaicin (lower panel). F. Quantification of β-cell mass (males). G. Glucose tolerance test (females). H. Insulin tolerance test (females). I. Glucose-stimulated insulin secretion (females). J. Representative fluorescence images of pancreases co-stained for BrdU (green), insulin (red) and DAPI (blue). Pancreases were harvested from female mice injected with vehicle (upper panel) or capsaicin (lower panel). K. Quantification of β-cell mass (females). Data represent mean ± SEM. ∗ p ≤ 0.05 and ∗∗ p ≤ 0.01 (n = 4–6 per group).

    Article Snippet: In this context, a recent study has revealed that testosterone binds and activates the transient receptor potential melastatin 8 (TRPM8) known to exhibit functional interactions with TRPV1 .

    Techniques: Cell Function Assay, Mouse Assay, Injection, Fluorescence, Staining

    Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Journal: Molecular Metabolism

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    doi: 10.1016/j.molmet.2018.10.002

    Figure Lengend Snippet: Whole-body chemical ablation of TRPV1 sensory neurons improves glucose tolerance in a sex-dependent manner : Five-week-old male and female C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Ten days post-treatment, mice were subjected to metabolic phenotyping tests. A. Schematic of the experimental design. B. Glucose tolerance test (males). C. Insulin tolerance test (males). D. Body weight (males). E. Random-fed blood glucose (males). F. Random-fed insulin levels (males). G. Lean mass evaluated by DEXA analysis (males). H. Quantification of fat mass by DEXA analysis (males). I. Glucose tolerance test (females). J. Insulin tolerance test (females). K. Body weight (females). L. Random-fed blood glucose (females). M. Random-fed insulin levels (females). N. Lean mass evaluated by DEXA analysis (females). O. Quantification of fat mass by DEXA analysis (females). Data represent mean ± SEM. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 (n = 6–7 per group).

    Article Snippet: In this context, a recent study has revealed that testosterone binds and activates the transient receptor potential melastatin 8 (TRPM8) known to exhibit functional interactions with TRPV1 .

    Techniques: Mouse Assay, Injection

    Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Journal: Molecular Metabolism

    Article Title: TRPV1 neurons regulate β-cell function in a sex-dependent manner

    doi: 10.1016/j.molmet.2018.10.002

    Figure Lengend Snippet: Whole-body TRPV1 sensory denervation does not affect energy expenditure in male mice : Five-week-old male C57BL/6J mice were subcutaneously injected into the scruff of the neck with capsaicin (50 mg/kg) or vehicle once per day for three consecutive days. Twenty-five days post-treatment, indirect calorimetric assays were performed using the Comprehensive Laboratory Animal Monitoring System (CLAMS). A. Locomotor activity (counts). B. Energy expenditure (kcal/hour) C. Oxygen consumption (ml/kg/hour). D. Carbon dioxide release (ml/kg/hour). E. Respiratory Exchange Ratio. F. Diurnal profiles of food intake (g).

    Article Snippet: In this context, a recent study has revealed that testosterone binds and activates the transient receptor potential melastatin 8 (TRPM8) known to exhibit functional interactions with TRPV1 .

    Techniques: Mouse Assay, Injection, Activity Assay

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VII. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VII region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice cerebellar lobule VI. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice cerebellar lobule VI region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVI. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice ventrolateral PAG. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice vlPAG region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice cerebellum CVII. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    TRPV1 and related molecular pathways in the mouse brain

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: TRPV1 and related molecular pathways in the mouse brain

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques:

    Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Immunofluorescence staining of TRPV1, Iba1, and double staining protein expression in the mice hypothalamus. A TRPV1, B Iba1, and C TRPV1/Iba1 double staining, immuno-positive (green, red, or yellow) signals in the mice hypothalamus region. Scale bar: 100 μm. n = 4 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Immunofluorescence, Staining, Double Staining, Expressing, Mouse Assay

    Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Mechanical withdrawal, thermal latency, and experimental flow in normal, CSP, EA, and Trpv1 −/− mice. A Mechanical threshold from the von Frey tests. B Thermal latency from the Hargreaves’ test. C Experimental flow in normal, CSP, EA, and Trpv1 −/− mice. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP groups. n = 10 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay

    Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice PAG. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Journal: Chinese Medicine

    Article Title: Electroacupuncture reduces cold stress-induced pain through microglial inactivation and transient receptor potential V1 in mice

    doi: 10.1186/s13020-021-00451-0

    Figure Lengend Snippet: Levels of TRPV1 and related molecules in the mice hypothalamus. The western blot bands contain four lanes of protein expression corresponding to the Normal, CSP, EA, and Trpv1 −/− groups. A TRPV1, B HMGB1, C S100B, D TLR4, E RAGE, F pPI3K, G pAkt, H pmTOR, I pERK, J pp38, K pJNK, and L pNFκB protein levels. *Indicates statistical significance when compared with the normal group. # Indicates statistical significance when compared with the CSP group. n = 6 in all groups

    Article Snippet: After blocking, the samples were incubated with the primary antibody (1:200, Alomone), TRPV1 and Iba1, prepared in 1% bovine serum albumin solution at 4 ºC overnight.

    Techniques: Mouse Assay, Western Blot, Expressing

    Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Journal: Chinese Medicine

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    doi: 10.1186/s13020-021-00468-5

    Figure Lengend Snippet: Effects of CSBTA on the protein expression of PKCε ( A ), p-p38 MAPK ( B ), and TRPV1 ( C ) and the TRPV1 mRNA levels ( D , F ) in paclitaxel-stimulated primary DRG neurons. The corresponding quantitative data were present as mean ± SD. n = 6. E Representative images of PKCε (green) and DAPI (blue) stained DRG neuron. Scale bar, 5 μm. # p

    Article Snippet: Western-blot and RT-qPCR analyzed the protein and mRNA expressions of PKCε, TRPV1, p-p38 MAPK from the spinal cord and DRG.

    Techniques: Expressing, Staining

    Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Journal: Chinese Medicine

    Article Title: Corydalis saxicola Bunting total alkaloids attenuate paclitaxel-induced peripheral neuropathy through PKCε/p38 MAPK/TRPV1 signaling pathway

    doi: 10.1186/s13020-021-00468-5

    Figure Lengend Snippet: Effects of CSBTA on PIPN-induced protein and gene expression in different tissues of rats. Representative Western blots of p-p38 MAPK (left), PKCε (middle), TRPV1 (right) expression in PIPN rats (n = 5). The gene expression of TRPV1 and PKCε in DRG ( D ) of PIPN rats (n = 6). The corresponding quantitative data were present as mean ± SD. # p

    Article Snippet: Western-blot and RT-qPCR analyzed the protein and mRNA expressions of PKCε, TRPV1, p-p38 MAPK from the spinal cord and DRG.

    Techniques: Expressing, Western Blot