tertiapinq  (Alomone Labs)


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    Alomone Labs tertiapinq
    Tertiapinq, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tertiapinq/product/Alomone Labs
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tertiapinq - by Bioz Stars, 2022-12
    91/100 stars

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    Alomone Labs tertiapin q tpq
    G i/o -GPCRs regulate human β-cell electrical excitability by stimulating NKA activity. A Top row: Representative immunofluorescent staining of a healthy human pancreatic section for NKA α1 subunits (green), insulin (red), and a merged image of the two showing colocalization (yellow; staining representative of pancreas sections from 4 human donors). Bottom row: Magnification of the corresponding areas outlined with yellow boxes above. B Representative human β-cell SST [Ca 2+ ] i responses (F/F min ; within intact human islets) at 7 mM glucose (7G). C Human β-cell [Ca 2+ ] i AUC (average of ≥15 min) at 7G before (white) and after SST (green; n = 4). D Human β-cell [Ca 2+ ] i plateau fraction at 7G before (white) and after SST (green; n = 4). E Representative human islet SST [Ca 2+ ] i responses with (top) and without extracellular K + (0 [K + ]; bottom). F SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) with (green) and without extracellular K + (red; n = 5). G Representative human islet SST [Ca 2+ ] i responses in the absence (top) and the presence of <t>tertiapin-Q</t> <t>(TPQ;</t> bottom). H SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) in the absence (green) and the presence of TPQ (blue; n = 3). I Representative human β-cell V m recording showing a typical SST response. Whole-cell β-cell currents were measured (indicated by arrows) in response to a voltage ramp protocol (see Fig. 1A inset). J Human β-cell V m before (white) and after SST (green; n = 7). K Normalized human β-cell currents ( I / I min ; I min = minimum current before SST) before (white) and after SST (green; n = 8). L Normalized human β-cell currents with 0 [K + ] before (white) and after SST (red; n = 5). M SST-induced human β-cell currents with (green; n = 8) and without extracellular K + (red; n = 5). Statistical analysis was conducted using paired two-sided two-sample t tests ( C , D , F , H , and J – L ) or an unpaired two-sided two-sample t test ( M ); * P
    Tertiapin Q Tpq, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tertiapin q tpq/product/Alomone Labs
    Average 94 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    tertiapin q tpq - by Bioz Stars, 2022-12
    94/100 stars
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    G i/o -GPCRs regulate human β-cell electrical excitability by stimulating NKA activity. A Top row: Representative immunofluorescent staining of a healthy human pancreatic section for NKA α1 subunits (green), insulin (red), and a merged image of the two showing colocalization (yellow; staining representative of pancreas sections from 4 human donors). Bottom row: Magnification of the corresponding areas outlined with yellow boxes above. B Representative human β-cell SST [Ca 2+ ] i responses (F/F min ; within intact human islets) at 7 mM glucose (7G). C Human β-cell [Ca 2+ ] i AUC (average of ≥15 min) at 7G before (white) and after SST (green; n = 4). D Human β-cell [Ca 2+ ] i plateau fraction at 7G before (white) and after SST (green; n = 4). E Representative human islet SST [Ca 2+ ] i responses with (top) and without extracellular K + (0 [K + ]; bottom). F SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) with (green) and without extracellular K + (red; n = 5). G Representative human islet SST [Ca 2+ ] i responses in the absence (top) and the presence of tertiapin-Q (TPQ; bottom). H SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) in the absence (green) and the presence of TPQ (blue; n = 3). I Representative human β-cell V m recording showing a typical SST response. Whole-cell β-cell currents were measured (indicated by arrows) in response to a voltage ramp protocol (see Fig. 1A inset). J Human β-cell V m before (white) and after SST (green; n = 7). K Normalized human β-cell currents ( I / I min ; I min = minimum current before SST) before (white) and after SST (green; n = 8). L Normalized human β-cell currents with 0 [K + ] before (white) and after SST (red; n = 5). M SST-induced human β-cell currents with (green; n = 8) and without extracellular K + (red; n = 5). Statistical analysis was conducted using paired two-sided two-sample t tests ( C , D , F , H , and J – L ) or an unpaired two-sided two-sample t test ( M ); * P

    Journal: Nature Communications

    Article Title: Gi/o protein-coupled receptor inhibition of beta-cell electrical excitability and insulin secretion depends on Na+/K+ ATPase activation

    doi: 10.1038/s41467-022-34166-z

    Figure Lengend Snippet: G i/o -GPCRs regulate human β-cell electrical excitability by stimulating NKA activity. A Top row: Representative immunofluorescent staining of a healthy human pancreatic section for NKA α1 subunits (green), insulin (red), and a merged image of the two showing colocalization (yellow; staining representative of pancreas sections from 4 human donors). Bottom row: Magnification of the corresponding areas outlined with yellow boxes above. B Representative human β-cell SST [Ca 2+ ] i responses (F/F min ; within intact human islets) at 7 mM glucose (7G). C Human β-cell [Ca 2+ ] i AUC (average of ≥15 min) at 7G before (white) and after SST (green; n = 4). D Human β-cell [Ca 2+ ] i plateau fraction at 7G before (white) and after SST (green; n = 4). E Representative human islet SST [Ca 2+ ] i responses with (top) and without extracellular K + (0 [K + ]; bottom). F SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) with (green) and without extracellular K + (red; n = 5). G Representative human islet SST [Ca 2+ ] i responses in the absence (top) and the presence of tertiapin-Q (TPQ; bottom). H SST-induced decrease in human islet [Ca 2+ ] i AUC (sum of 5 min; relative to before treatment) in the absence (green) and the presence of TPQ (blue; n = 3). I Representative human β-cell V m recording showing a typical SST response. Whole-cell β-cell currents were measured (indicated by arrows) in response to a voltage ramp protocol (see Fig. 1A inset). J Human β-cell V m before (white) and after SST (green; n = 7). K Normalized human β-cell currents ( I / I min ; I min = minimum current before SST) before (white) and after SST (green; n = 8). L Normalized human β-cell currents with 0 [K + ] before (white) and after SST (red; n = 5). M SST-induced human β-cell currents with (green; n = 8) and without extracellular K + (red; n = 5). Statistical analysis was conducted using paired two-sided two-sample t tests ( C , D , F , H , and J – L ) or an unpaired two-sided two-sample t test ( M ); * P

    Article Snippet: Tertiapin-Q (TPQ) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay, Staining

    SSTR-mediated β-cell currents are not due to GIRK channel activation. A Representative WT β-cell V m recording showing a typical SST response in the presence of 20 mM glucose (20G) + tolbutamide (Tolb). Whole-cell β-cell currents were measured (indicated by arrows) in response to a voltage ramp protocol (inset). B WT β-cell currents before (white) and after SST (green; n = 11). C SST-induced WT β-cell currents (green; n = 11). D WT β-cell V m before (white) and after SST (green; n = 13). E GIRK2 KO Panc β-cell currents before (dark blue) and after SST (green; n = 4). F SST-induced GIRK2 KO Panc β-cell currents (green; n = 4). G GIRK2 KO Panc β-cell V m before (dark blue) and after SST (green; n = 4). H Representative WT islet [Ca 2+ ] i response (F/F min ) to Tolb and 20G. I Representative WT islet SST [Ca 2+ ] i response. J Representative GIRK2 KO Panc islet SST [Ca 2+ ] i responses. K Percentage of WT islets (white; n = 6) and GIRK2 KO Panc islets (dark blue; n = 3) displaying SST-induced [Ca 2+ ] i oscillations. L GIRK2 KO Panc islet [Ca 2+ ] i plateau fraction before (dark blue) and after SST (green; n = 3). M Representative WT β-cell V m recording showing typical tertiapin-Q (TPQ) and SST responses. N WT β-cell currents before treatment (white), after TPQ (pink), and after TPQ + SST (blue; n = 8). O TPQ-induced (pink) and TPQ + SST-induced WT β-cell currents (green; n = 8). P WT β-cell V m before treatment (white), after TPQ (pink), and after TPQ + SST (blue; n = 8). Q Representative WT islet SST and TPQ [Ca 2+ ] i responses. R Percentage of WT islets displaying SST-induced [Ca 2+ ] i oscillations before (green) and after TPQ (blue; n = 4). S WT islet [Ca 2+ ] i plateau fraction before treatment (white), after SST (green), and after SST + TPQ (blue; n = 4). Statistical analysis was conducted using paired two-sided two-sample t tests ( B , D , E , G , O ), unpaired two-sided two-sample t tests ( K , L , R ), one-sample t tests ( C , F ), or one-way ANOVA with Šidák’s post-hoc multiple comparisons tests ( N , P , S ); * P

    Journal: Nature Communications

    Article Title: Gi/o protein-coupled receptor inhibition of beta-cell electrical excitability and insulin secretion depends on Na+/K+ ATPase activation

    doi: 10.1038/s41467-022-34166-z

    Figure Lengend Snippet: SSTR-mediated β-cell currents are not due to GIRK channel activation. A Representative WT β-cell V m recording showing a typical SST response in the presence of 20 mM glucose (20G) + tolbutamide (Tolb). Whole-cell β-cell currents were measured (indicated by arrows) in response to a voltage ramp protocol (inset). B WT β-cell currents before (white) and after SST (green; n = 11). C SST-induced WT β-cell currents (green; n = 11). D WT β-cell V m before (white) and after SST (green; n = 13). E GIRK2 KO Panc β-cell currents before (dark blue) and after SST (green; n = 4). F SST-induced GIRK2 KO Panc β-cell currents (green; n = 4). G GIRK2 KO Panc β-cell V m before (dark blue) and after SST (green; n = 4). H Representative WT islet [Ca 2+ ] i response (F/F min ) to Tolb and 20G. I Representative WT islet SST [Ca 2+ ] i response. J Representative GIRK2 KO Panc islet SST [Ca 2+ ] i responses. K Percentage of WT islets (white; n = 6) and GIRK2 KO Panc islets (dark blue; n = 3) displaying SST-induced [Ca 2+ ] i oscillations. L GIRK2 KO Panc islet [Ca 2+ ] i plateau fraction before (dark blue) and after SST (green; n = 3). M Representative WT β-cell V m recording showing typical tertiapin-Q (TPQ) and SST responses. N WT β-cell currents before treatment (white), after TPQ (pink), and after TPQ + SST (blue; n = 8). O TPQ-induced (pink) and TPQ + SST-induced WT β-cell currents (green; n = 8). P WT β-cell V m before treatment (white), after TPQ (pink), and after TPQ + SST (blue; n = 8). Q Representative WT islet SST and TPQ [Ca 2+ ] i responses. R Percentage of WT islets displaying SST-induced [Ca 2+ ] i oscillations before (green) and after TPQ (blue; n = 4). S WT islet [Ca 2+ ] i plateau fraction before treatment (white), after SST (green), and after SST + TPQ (blue; n = 4). Statistical analysis was conducted using paired two-sided two-sample t tests ( B , D , E , G , O ), unpaired two-sided two-sample t tests ( K , L , R ), one-sample t tests ( C , F ), or one-way ANOVA with Šidák’s post-hoc multiple comparisons tests ( N , P , S ); * P

    Article Snippet: Tertiapin-Q (TPQ) was purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activation Assay

    Oxidative stress results in increased I KACh via translocation of PKCε (A and B) Representative TIRFM images showing recruitment of mCherry-CRY2-mPKCεCAT-HA to the cell surface by 100 μM H 2 O 2 . Scale bar: 10 μm (B) Summary data showing cell surface localization of mCherry-CRY2-mPKCεCAT-HA at the cell surface following treatment with H 2 O 2 (n = 10 cells for baseline, n = 12 cells for the 5 min, n = 24 cells for the 15 min, and n = 16 cells for the 30 min time points). (C) IV curves of basal I KACh (tertiapin sensitive current) control (black), H 2 O 2 treated (red), and PKCε silenced and H 2 O 2 treated (green), as measured in HEK293 cells stably expressing Kir3.1 and Kir3.4, and co-transfected with PKCε SiRNA and GFP. (D) Quantification of maximum inward current. n = 3 cells for control, n = 6 cells for H 2 O 2 , n = 6 cells for H 2 O 2 +SiRNA, and n = 5 cells for SiRNA alone. Shapiro-Wilk test confirmed normality of data. ∗p

    Journal: iScience

    Article Title: IKACh is constitutively active via PKC epsilon in aging mediated atrial fibrillation

    doi: 10.1016/j.isci.2022.105442

    Figure Lengend Snippet: Oxidative stress results in increased I KACh via translocation of PKCε (A and B) Representative TIRFM images showing recruitment of mCherry-CRY2-mPKCεCAT-HA to the cell surface by 100 μM H 2 O 2 . Scale bar: 10 μm (B) Summary data showing cell surface localization of mCherry-CRY2-mPKCεCAT-HA at the cell surface following treatment with H 2 O 2 (n = 10 cells for baseline, n = 12 cells for the 5 min, n = 24 cells for the 15 min, and n = 16 cells for the 30 min time points). (C) IV curves of basal I KACh (tertiapin sensitive current) control (black), H 2 O 2 treated (red), and PKCε silenced and H 2 O 2 treated (green), as measured in HEK293 cells stably expressing Kir3.1 and Kir3.4, and co-transfected with PKCε SiRNA and GFP. (D) Quantification of maximum inward current. n = 3 cells for control, n = 6 cells for H 2 O 2 , n = 6 cells for H 2 O 2 +SiRNA, and n = 5 cells for SiRNA alone. Shapiro-Wilk test confirmed normality of data. ∗p

    Article Snippet: To measure the constitutively active IKACh current in atrial cardiomyocytes, 100 nM Tertiapin-Q (TPQ, Alomone Labs) was applied to the extracellular solution.

    Techniques: Translocation Assay, Stable Transfection, Expressing, Transfection