tertiapinq atto 488  (Alomone Labs)


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    Alomone Labs tertiapinq atto 488
    Determination of the IC 50 for chloroquine and <t>tertiapinQ</t> block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) <t>ATTO-488</t> does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.
    Tertiapinq Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tertiapinq atto 488/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tertiapinq atto 488 - by Bioz Stars, 2022-01
    91/100 stars

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    1) Product Images from "Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule"

    Article Title: Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule

    Journal: The FASEB Journal

    doi: 10.1096/fj.201700349R

    Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.
    Figure Legend Snippet: Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.

    Techniques Used: Blocking Assay, Stable Transfection, Transfection, Confocal Microscopy, Fluorescence, Staining, Expressing

    F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P
    Figure Legend Snippet: F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P

    Techniques Used: Blocking Assay, Confocal Microscopy, Fluorescence, Staining, Transfection

    2) Product Images from "Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule"

    Article Title: Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule

    Journal: The FASEB Journal

    doi: 10.1096/fj.201700349R

    Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.
    Figure Legend Snippet: Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.

    Techniques Used: Blocking Assay, Stable Transfection, Transfection, Confocal Microscopy, Fluorescence, Staining, Expressing

    F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P
    Figure Legend Snippet: F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P

    Techniques Used: Blocking Assay, Confocal Microscopy, Fluorescence, Staining, Transfection

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    • tertiapinq atto 488  (Alomone Labs)
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    Alomone Labs tertiapinq atto 488
    Determination of the IC 50 for chloroquine and <t>tertiapinQ</t> block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) <t>ATTO-488</t> does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.
    Tertiapinq Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tertiapinq atto 488/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tertiapinq atto 488 - by Bioz Stars, 2022-01
    91/100 stars
    		  Buy from Supplier

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    Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.

    Journal: The FASEB Journal

    Article Title: Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule

    doi: 10.1096/fj.201700349R

    Figure Lengend Snippet: Determination of the IC 50 for chloroquine and tertiapinQ block of I KACh in HEK293 cells stably transfected with Kir3.1 and Kir3.4. A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Upper: Live HEK293 cells transfected with mCherry. TertiapinQ (TPQ) ATTO-488 does not show staining. Lower: HEK293 cells, transfected with mCherry and stably expressing Kir3.1/Kir3.4, show robust staining of the cell membranes by tertiapinQ ATTO-488. Laser and detector settings were identical in both cases. B ) I KACh recorded in HEK293 cells expressing Kir3.1/3.4 in response to a ramp in the absence and the presence of 1 μM chloroquine (CQ) or 0.1 μM tertiapinQ. C ) Dose-response curve of I KACh block by chloroquine, IC 50 = 1.02 μM, R 2 = 0.96; TertiapinQ, IC 50 = 0.07 μM, R 2 = 0.94.

    Article Snippet: For fluorescence imaging, cells were plated on a coverslip and stained for 10 s with tertiapinQ-ATTO-488 (Alomone Laboratories, Jerusalem, Israel), and then images were acquired using an Olympus FV1000 multiphoton laser-scanning microscope.

    Techniques: Blocking Assay, Stable Transfection, Transfection, Confocal Microscopy, Fluorescence, Staining, Expressing

    F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P

    Journal: The FASEB Journal

    Article Title: Structural basis for the antiarrhythmic blockade of a potassium channel with a small molecule

    doi: 10.1096/fj.201700349R

    Figure Lengend Snippet: F255A and D260A mutations reduce the ability of chloroquine to block I KACh . A ) Confocal microscopy. Fluorescence staining of I KACh proteins. Top) Live HEK293 cells transfected with mCherry, WT Kir3.1, and Kir3.4. Middle (bottom): cells transfected with mCherry, Kir3.1 F255A or D260A, and Kir3.4, where tertiapinQ ATTO-488 showed robust staining of the cell membranes. B ) BaCl 2 -sensitive I KACh currents elicited in WT Kir.31/Kir3.4-, F255A Kir3.1/Kir3.4-, or D260A Kir3.1/Kir3.4-transfected cells, in response ramps from −140 to +30 mV, before and after addition of 1 μM CQ. C, left: Dose-response curves for the effect of chloroquine on the BaCl 2 -sensitive inward current measured at −120 mV. IC 50 for WT: 0.8 μM, R 2 = 0.81, n = 11; F255A: 3.2 μM, R 2 = 0.75, n = 10; and D260: 2.8 μM, R 2 = 0.9, n = 10. P

    Article Snippet: For fluorescence imaging, cells were plated on a coverslip and stained for 10 s with tertiapinQ-ATTO-488 (Alomone Laboratories, Jerusalem, Israel), and then images were acquired using an Olympus FV1000 multiphoton laser-scanning microscope.

    Techniques: Blocking Assay, Confocal Microscopy, Fluorescence, Staining, Transfection