imperatoxin a  (Alomone Labs)


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  • 85
    Name:
    Imperatoxin A
    Description:
    Imperatoxin A is a strong agonist of the Ryanodine receptor channels RyR Induces voltage and concentration dependent subconductance states in both skeletal RYR1 and RYR3 and cardiac RYR2 Ryanodine receptors by binding to a single cytosolic accessible site different from the ryanodine binding site The activating effect is dose dependent ED50 10 nM with fast onset and found fully reversible
    Catalog Number:
    STI-333
    Price:
    199.0
    Category:
    Toxin
    Source:
    Synthetic peptide
    Applications:
    0
    Purity:
    >98% (HPLC)
    Size:
    0 1 mg
    Format:
    Lyophilized powder.
    Formula:
    C148H254N58O45S6
    Molecular Weight:
    3758 Da.
    Molecule Name:
    Imperatoxin A, IpTxa
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    Structured Review

    Alomone Labs imperatoxin a
    Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM <t>imperatoxin</t> A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.
    Imperatoxin A is a strong agonist of the Ryanodine receptor channels RyR Induces voltage and concentration dependent subconductance states in both skeletal RYR1 and RYR3 and cardiac RYR2 Ryanodine receptors by binding to a single cytosolic accessible site different from the ryanodine binding site The activating effect is dose dependent ED50 10 nM with fast onset and found fully reversible
    https://www.bioz.com/result/imperatoxin a/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imperatoxin a - by Bioz Stars, 2021-09
    85/100 stars

    Images

    1) Product Images from "Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine"

    Article Title: Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0008315

    Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM imperatoxin A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.
    Figure Legend Snippet: Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM imperatoxin A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.

    Techniques Used: Activity Assay

    Effect of protamine in combination with imperatoxin A (IpTx A ). RyR2 channels fully activated by the combined action of cytosolic Ca 2+ (10 µM) and caffeine (5 mM) were used as control ( A ). Subsequently, 50 nM IpTx A ( B ) or 1 µg/ml protamine ( C ) were added. To test the combined effect of 1 µg/ml protamine + 50 nM IpTx A two sets of experiments were performed: one adding protamine first and then IpTx A and the other, switching the order in which these agents were added. The results were the same in both sets of experiments. A representative recording is displayed in panel ( D ). All traces shown were performed at V m = +20 mV. Current levels for the baseline ( b ), full open state (o), protamine-induced substate ( p ) are indicated. A small substate ( s ) is indicated. In panels ( A ) and ( B ) the amplitude of this substate is ∼30% of the full open state and in panels ( C ) and ( D ) it is ∼30% the amplitude of the protamine-induced substate. ( E ) Probability of substates at the s level (P S ) as a function of holding voltage of RyR2 channels under control conditions (open triangles), exposed to 50 nM IpTx A (open circles), 1 µg/ml protamine (filled triangles) and 50 nM IpTx A +1 µg/ml protamine (filled circles). Values are means±SEM (* P
    Figure Legend Snippet: Effect of protamine in combination with imperatoxin A (IpTx A ). RyR2 channels fully activated by the combined action of cytosolic Ca 2+ (10 µM) and caffeine (5 mM) were used as control ( A ). Subsequently, 50 nM IpTx A ( B ) or 1 µg/ml protamine ( C ) were added. To test the combined effect of 1 µg/ml protamine + 50 nM IpTx A two sets of experiments were performed: one adding protamine first and then IpTx A and the other, switching the order in which these agents were added. The results were the same in both sets of experiments. A representative recording is displayed in panel ( D ). All traces shown were performed at V m = +20 mV. Current levels for the baseline ( b ), full open state (o), protamine-induced substate ( p ) are indicated. A small substate ( s ) is indicated. In panels ( A ) and ( B ) the amplitude of this substate is ∼30% of the full open state and in panels ( C ) and ( D ) it is ∼30% the amplitude of the protamine-induced substate. ( E ) Probability of substates at the s level (P S ) as a function of holding voltage of RyR2 channels under control conditions (open triangles), exposed to 50 nM IpTx A (open circles), 1 µg/ml protamine (filled triangles) and 50 nM IpTx A +1 µg/ml protamine (filled circles). Values are means±SEM (* P

    Techniques Used:

    Related Articles

    Modification:

    Article Title: Insights into the Gating Mechanism of the Ryanodine-Modified Human Cardiac Ca2+-Release Channel (Ryanodine Receptor 2)
    Article Snippet: .. In our experiments, the channels were modified using ryanodine (Abcam Biochemicals, Cambridge, UK) and synthetic IpTxa (Alomone Laboratories, Jerusalem, Israel), whereas their gating behavior was examined under contaminant (~1 μM) or virtually zero Ca2+ conditions (extrapolated to ~20 pM free Ca2+ as per ) using 3.5 mM EGTA on both cis/trans sides (the pH remained unaltered at 7.4). ..

    Article Title: Insights into the Gating Mechanism of the Ryanodine-Modified Human Cardiac Ca2+-Release Channel (Ryanodine Receptor 2)
    Article Snippet: .. As in earlier experiments, channels were modified with 1 μ M ryanodine and Ca2+ removed, before being further modified with 20 nM synthetic IpTxa added to the cytosolic side ( ). ..

    other:

    Article Title: Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine
    Article Snippet: Imperatoxin A (IpTxA ) was from Alomone Labs (Jerusalem, Israel).

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  • 85
    Alomone Labs imperatoxin a
    Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM <t>imperatoxin</t> A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.
    Imperatoxin A, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imperatoxin a/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    imperatoxin a - by Bioz Stars, 2021-09
    85/100 stars
      Buy from Supplier

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    Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM imperatoxin A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.

    Journal: PLoS ONE

    Article Title: Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine

    doi: 10.1371/journal.pone.0008315

    Figure Lengend Snippet: Effect of protamine compared to other conductance-modifiers. Single-channel recordings of fully active RyR2 channels (10 µM cytosolic Ca 2+ /5 mM caffeine) in the presence of 1 µg/ml protamine, 10 µM ryanodol or 50 nM imperatoxin A ( A , B and C , respectively). Channel activity was recorded at V m = 0 mV (top traces) and +40 mV (bottom traces). The current levels for the baseline, full open state and substates induced by protamine, ryanodol and IpTx A are indicated ( b, o, p, r and i , respectively). ( D ) Current amplitude as a function of voltage for the full open state (n = 28) and for substates induced by protamine (n = 10), ryanodol (n = 13) and IpTx A (n = 5). Slope conductances were, in pS, 129±1, 123±3, 56±2 and 43±3, respectively.

    Article Snippet: Imperatoxin A (IpTxA ) was from Alomone Labs (Jerusalem, Israel).

    Techniques: Activity Assay

    Effect of protamine in combination with imperatoxin A (IpTx A ). RyR2 channels fully activated by the combined action of cytosolic Ca 2+ (10 µM) and caffeine (5 mM) were used as control ( A ). Subsequently, 50 nM IpTx A ( B ) or 1 µg/ml protamine ( C ) were added. To test the combined effect of 1 µg/ml protamine + 50 nM IpTx A two sets of experiments were performed: one adding protamine first and then IpTx A and the other, switching the order in which these agents were added. The results were the same in both sets of experiments. A representative recording is displayed in panel ( D ). All traces shown were performed at V m = +20 mV. Current levels for the baseline ( b ), full open state (o), protamine-induced substate ( p ) are indicated. A small substate ( s ) is indicated. In panels ( A ) and ( B ) the amplitude of this substate is ∼30% of the full open state and in panels ( C ) and ( D ) it is ∼30% the amplitude of the protamine-induced substate. ( E ) Probability of substates at the s level (P S ) as a function of holding voltage of RyR2 channels under control conditions (open triangles), exposed to 50 nM IpTx A (open circles), 1 µg/ml protamine (filled triangles) and 50 nM IpTx A +1 µg/ml protamine (filled circles). Values are means±SEM (* P

    Journal: PLoS ONE

    Article Title: Voltage-Dependent Modulation of Cardiac Ryanodine Receptors (RyR2) by Protamine

    doi: 10.1371/journal.pone.0008315

    Figure Lengend Snippet: Effect of protamine in combination with imperatoxin A (IpTx A ). RyR2 channels fully activated by the combined action of cytosolic Ca 2+ (10 µM) and caffeine (5 mM) were used as control ( A ). Subsequently, 50 nM IpTx A ( B ) or 1 µg/ml protamine ( C ) were added. To test the combined effect of 1 µg/ml protamine + 50 nM IpTx A two sets of experiments were performed: one adding protamine first and then IpTx A and the other, switching the order in which these agents were added. The results were the same in both sets of experiments. A representative recording is displayed in panel ( D ). All traces shown were performed at V m = +20 mV. Current levels for the baseline ( b ), full open state (o), protamine-induced substate ( p ) are indicated. A small substate ( s ) is indicated. In panels ( A ) and ( B ) the amplitude of this substate is ∼30% of the full open state and in panels ( C ) and ( D ) it is ∼30% the amplitude of the protamine-induced substate. ( E ) Probability of substates at the s level (P S ) as a function of holding voltage of RyR2 channels under control conditions (open triangles), exposed to 50 nM IpTx A (open circles), 1 µg/ml protamine (filled triangles) and 50 nM IpTx A +1 µg/ml protamine (filled circles). Values are means±SEM (* P

    Article Snippet: Imperatoxin A (IpTxA ) was from Alomone Labs (Jerusalem, Israel).

    Techniques: