ω Conotoxin Cnviia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization"
Article Title: Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization
Journal: Development & Reproduction
Figure Legend Snippet: Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on fertilization and embryonic development. Mature eggs were incubated for 1 hr with ω-Conotoxin CnVIIA and fertilized with BDF1 sperm in the same medium for 8 h. (A) Two pronuclear zygotes were collected and cultured in KSOM for five days. (B) Fertilization rates were calculated at 2 cells per MII mature eggs, and the blastocyst rate were calculated from 2-cells. * p
Techniques Used: Incubation, Cell Culture
Figure Legend Snippet: Effect of N-type voltage dependent calcium channel (VDCC) blocker, ω-Conotoxin CnVIIA, on [Ca 2+ ]i oscillation by SrCl egg activation. (A) 10 mM SrCl in [Ca 2+ ]i free TL-HEPES medium. Parenthesis in each group represents egg number /total egg number. (B) Relative fluorescence intensity was calculated using the basal level of [Ca 2+ ] and the amplitude of [Ca 2+ ]i spikes (max-min). The number of spikes per 2 h and duration of each spike were determined from (A). Each dot represents the number of eggs.
Techniques Used: Activation Assay, Fluorescence