chlorotoxin  (Alomone Labs)

Journal: International Journal of Molecular Sciences

Article Title: The Effectiveness in Activating M-Type K+ Current Produced by Solifenacin ([(3R)-1-azabicyclo[2.2.2]octan-3-yl] (1S)-1-phenyl-3,4-dihydro-1H-isoquinoline-2-carboxylate): Independent of Its Antimuscarinic Action

doi: 10.3390/ijms222212399

Figure Lengend Snippet: Effect of SOL, SOL plus acetylcholine (ACh), SOL plus iberiotoxin (Iber), SOL plus apamin (Apa), SOL plus tolbutamide (TLB), SOL plus chlorotoxin (ChTx), SOL plus Lino, or SOL plus TRH on the amplitude of I K(M) . In these experiments, we bathed GH 3 cells in high-K + , Ca 2+ -free solution and the recording electrode was filled with K + -enriched (145 mM) solution. Current amplitude was measured at the end of the depolarizing step from −50 to −10 mV. Each bar represents the mean ± SEM ( n = 7). * Significantly different from controls ( p

Article Snippet: Chlorotoxin (ChTx) was kindly provided by Professor Dr. Woei-Jer Chuang (Department of Biochemistry, National Cheng Kung University Medical College, Tainan, Taiwan).

Techniques:

Journal: Biomaterials

Article Title: A ligand-mediated nanovector for targeted gene delivery and transfection in cancer cells

doi: 10.1016/j.biomaterials.2008.10.003

Figure Lengend Snippet: 1 H NMR spectra showing characteristic peaks of the chlorotoxin peptide (CTX) at 2.60–3.10 and 3.65–3.80 ppm, SIAX-functionalized P-PEG polymer (P-PEG-SIAX) at 3.60–3.65 for the -O-CH2-CH2- repeating unit of PEG and at 2.50–3.10

Article Snippet: Chlorotoxin (Alomone Labs, Ltd. Jerusalem, Israel) is a peptide derived from vemon of the Giant Israeli scorpion Leiurus q. hebraeus .

Techniques: Nuclear Magnetic Resonance

Journal: Biomaterials

Article Title: A ligand-mediated nanovector for targeted gene delivery and transfection in cancer cells

doi: 10.1016/j.biomaterials.2008.10.003

Figure Lengend Snippet: Nanovector preparation scheme. (a) PEGylation of PEI polymer and modification with Alexa Fluor 647 fluorophores (AF). (b) P-PEG-AF modification with SIAX, modification of chlorotoxin (CTX) with traut’s reagent to produce free thiols on peptide

Article Snippet: Chlorotoxin (Alomone Labs, Ltd. Jerusalem, Israel) is a peptide derived from vemon of the Giant Israeli scorpion Leiurus q. hebraeus .

Techniques: Modification

Journal: ACS nano

Article Title: Chlorotoxin Labeled Magnetic Nanovectors for Targeted Gene Delivery to Glioma

doi: 10.1021/nn1008512

Figure Lengend Snippet: Enhanced delivery of GFP encoding DNA to C6 glioma cells in vivo using chlorotoxin labeled NPs. a) Xenogen images of tumors, livers, kidneys, and spleens from C6 xenograft tumor bearing mice, harvested 48 hrs after treatment, indicating GFP fluorescence

Article Snippet: NP-CP-PEI (herein called NP) were complexed with pEGFP-CS2 at a weight ratio of 10:1 (Fe equivalent of NP:DNA) in reaction buffer (20 mM HEPES, 5 mM EDTA, pH 7.2) for 30 min. Chlorotoxin (CTX, Alamone Labs, Jerusalem, Israel) was conjugated to DNA loaded NPs using a heterobifunctional PEG linker.

Techniques: In Vivo, Labeling, Mouse Assay, Fluorescence

Journal:

Article Title: Specific targeting of brain tumors with an optical/MR imaging nanoprobe across the blood brain barrier

doi: 10.1158/0008-5472.CAN-09-1157

Figure Lengend Snippet: In vivo NIRF imaging of autochthonous medulloblastoma tumors in genetically engineered ND2:SmoA1 mice. ( a and b ) Fluorescence imaging of medulloblastoma tumors in ND2:SmoA1 mice injected with either NPCP-Cy5.5-CTX or NPCP-Cy5.5, or receiving no injection

Article Snippet: CTX (Alamone Labs, Jerusalem, Israel) and Cy5.5 (GE Healthcare, Piscataway, NJ) were conjugated to the NPCP through the chemical scheme outlined in .

Techniques: In Vivo, Imaging, Mouse Assay, Fluorescence, Injection

Journal:

Article Title: Specific targeting of brain tumors with an optical/MR imaging nanoprobe across the blood brain barrier

doi: 10.1158/0008-5472.CAN-09-1157

Figure Lengend Snippet: Histological examination of mouse cerebellum 5 days post injection of NPCP-Cy5.5-CTX or NPCP-Cy5.5. ( a ) The H E stained cerebellum section of symptomatic ND2:SmoA1 mice confirming presence of medulloblastoma, and ( b ) for comparison, the cerebellum

Article Snippet: CTX (Alamone Labs, Jerusalem, Israel) and Cy5.5 (GE Healthcare, Piscataway, NJ) were conjugated to the NPCP through the chemical scheme outlined in .

Techniques: Injection, Staining, Mouse Assay

Journal:

Article Title: Specific targeting of brain tumors with an optical/MR imaging nanoprobe across the blood brain barrier

doi: 10.1158/0008-5472.CAN-09-1157

Figure Lengend Snippet: Biodistribution of nanoprobes. Accumulation of nanoprobes in various tissues assessed by NIRF signal measurements of tissues/organs excised from ND2:SmoA1 mice receiving no injection and 120 hours after receiving injection of either targeting NPCP-Cy5.5-CTX

Article Snippet: CTX (Alamone Labs, Jerusalem, Israel) and Cy5.5 (GE Healthcare, Piscataway, NJ) were conjugated to the NPCP through the chemical scheme outlined in .

Techniques: Mouse Assay, Injection

Journal:

Article Title: Specific targeting of brain tumors with an optical/MR imaging nanoprobe across the blood brain barrier

doi: 10.1158/0008-5472.CAN-09-1157

Figure Lengend Snippet: Synthesis and characterization of NPCP-Cy5.5-CTX nanoprobes. Chemical reaction schematic for the syntheses of ( a ) PEG-grafted chitosan, ( b ) sulfhydryl functionalization of chlorotoxin (CTX), and ( c ) chlorotoxin and Cy5.5 conjugation to NPCP. ( d ) Summary

Article Snippet: CTX (Alamone Labs, Jerusalem, Israel) and Cy5.5 (GE Healthcare, Piscataway, NJ) were conjugated to the NPCP through the chemical scheme outlined in .

Techniques: Conjugation Assay