staphylococcal  (Worthington Biochemical)


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  • 94
    Name:
    Protease S Aureus Endoproteinase Glu C
    Description:
    Chromatographically purified according to Drapeau G Boily Y and Houmard J J Biol Chem 247 6720 1972 A lyophilized powder
    Catalog Number:
    LS003605
    Price:
    228
    Source:
    S. aureus V8
    Size:
    5 mg
    Cas Number:
    66676.43.5
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    Structured Review

    Worthington Biochemical staphylococcal
    Chromatographically purified according to Drapeau G Boily Y and Houmard J J Biol Chem 247 6720 1972 A lyophilized powder
    https://www.bioz.com/result/staphylococcal/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    staphylococcal - by Bioz Stars, 2021-04
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: .. Smc3-3V5 or smc3-L217P-3V5 were precipitated from asynchronous cell cultures and incubated with V8 protease, with or without ATP. .. The reaction products were analyzed by western blot with antibodies against V5.

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: Chromatin immunoprecipitation (ChIP) Chromatin immunoprecipitation (ChIP) was performed as described ( ). .. Limited proteolysis Immunoprecipitated Smc3-3V5, either wild-type or L217P, were incubated in 20 μl of buffer (50 mM Tris–HCl (pH 8.0) and 1 mM MgCl2 ) with 5 mg/ml V8 protease (Worthington Biochemical), with or without 1 mM ATP (Sigma). ..

    Modification:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: .. Modified trypsin was from Promega (Madison, WI); Glu-C protease was from Worthington Biochemicals (Lakewood, NJ). .. Urea, Tris-HCl, CaCl2 , ammonium bicarbonate (NH4 HCO3 ), sodium fluoride (NaCl), potassium fluoride (KCl), potassium phosphate (KH2 PO4 ), phosphoric acid, sodium ortho-vanadate, sodium molybdate, sodium tartrate, beta-glycerophosphate, DL-dithiothreitol, iodoacetamide were from Sigma-Aldrich (St. Louis, MO).

    other:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Immunoprecipitation:

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: Chromatin immunoprecipitation (ChIP) Chromatin immunoprecipitation (ChIP) was performed as described ( ). .. Limited proteolysis Immunoprecipitated Smc3-3V5, either wild-type or L217P, were incubated in 20 μl of buffer (50 mM Tris–HCl (pH 8.0) and 1 mM MgCl2 ) with 5 mg/ml V8 protease (Worthington Biochemical), with or without 1 mM ATP (Sigma). ..

    Mass Spectrometry:

    Article Title: Matrix metalloproteinases inactivate the proinflammatory functions of secreted moonlighting tryptophanyl-tRNA synthetase
    Article Snippet: .. Samples were mixed, split in half, and digested for 16 h at 37 °C with either MS-grade trypsin (1 μg/ml; Thermo Fisher Scientific) or Glu-C ( Staphylococcus aureus protease V8; 1 μg/ml; Worthington). ..

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  • 94
    Worthington Biochemical staphylococcal
    Staphylococcal, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/staphylococcal/product/Worthington Biochemical
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    staphylococcal - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

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