staphylococcal  (Worthington Biochemical)


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    Structured Review

    Worthington Biochemical staphylococcal
    Staphylococcal, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/staphylococcal/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    staphylococcal - by Bioz Stars, 2020-11
    86/100 stars

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    Modification:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: .. Modified trypsin was from Promega (Madison, WI); Glu-C protease was from Worthington Biochemicals (Lakewood, NJ). .. Urea, Tris-HCl, CaCl2 , ammonium bicarbonate (NH4 HCO3 ), sodium fluoride (NaCl), potassium fluoride (KCl), potassium phosphate (KH2 PO4 ), phosphoric acid, sodium ortho-vanadate, sodium molybdate, sodium tartrate, beta-glycerophosphate, DL-dithiothreitol, iodoacetamide were from Sigma-Aldrich (St. Louis, MO).

    Protease Inhibitor:

    Article Title: Structural and mechanistic insights into ATRX-dependent and -independent functions of the histone chaperone DAXX
    Article Snippet: .. Nuclei were resuspended in 6 ml of buffer AC (15 mM HEPES pH 7.9, 30 mM KCl, 3 mM CaCl2 , 1 mM DTT, 0.4 mM PMSF, and 2× protease inhibitor cocktail) and incubated with Micrococcal Nuclease (MNase) (Worthington) at a final concentration of 25 U ml−1 for 20 min. ..

    Incubation:

    Article Title: Structural and mechanistic insights into ATRX-dependent and -independent functions of the histone chaperone DAXX
    Article Snippet: .. Nuclei were resuspended in 6 ml of buffer AC (15 mM HEPES pH 7.9, 30 mM KCl, 3 mM CaCl2 , 1 mM DTT, 0.4 mM PMSF, and 2× protease inhibitor cocktail) and incubated with Micrococcal Nuclease (MNase) (Worthington) at a final concentration of 25 U ml−1 for 20 min. ..

    Article Title: Remodeling of Yeast CUP1 Chromatin Involves Activator-Dependent Repositioning of Nucleosomes over the Entire Gene and Flanking Sequences
    Article Snippet: .. To prepare core particles, 25 to 40 ng of TAC minichromosomes were incubated in 40 μl of 10 mM Tris-HCl (pH 8.0)–35 mM NaCl–3 mM CaCl2 , with 2 to 4 U of micrococcal nuclease (MNase) (Worthington) for 2 min at 30°C, and EDTA was added to 5 mM. .. Core particle DNA was extracted, purified from 3% agarose gels, and end labeled with T4 kinase.

    Article Title: RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing
    Article Snippet: .. Cells were incubated with 1.0 mM DSP for 30 min at room temperature according to manufacturer’s instruction (Pierce), followed by crosslinking with formaldehyde for 10 min. Dual crosslinked chromatin was processed to mononucleosomes by micrococcal nuclease (MNase) (Worthington Biochemical Corporation) digestion (2.5 U of MNase/A260 of nuclear suspension) for 40 min at 37°C ( ). .. ChIPs were done with anti-RNAPIIS2ph (Abcam), anti-HDAC2 (Affinity Bioreagents), anti-SRSF1 (Santa Cruz Biotechnology), anti-H3K14ac (Abcam), anti-H3K9ac (Abcam), anti-acH3 (Millipore), anti-H4K5ac (Millipore), anti-H4K8ac (Millipore), anti-acH4 (Millipore), anti-H3 (Millipore), anti-KAT2B (Abcam) or anti-KAT7 (Santa Cruz Biotechnology) antibodies.

    other:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Concentration Assay:

    Article Title: Structural and mechanistic insights into ATRX-dependent and -independent functions of the histone chaperone DAXX
    Article Snippet: .. Nuclei were resuspended in 6 ml of buffer AC (15 mM HEPES pH 7.9, 30 mM KCl, 3 mM CaCl2 , 1 mM DTT, 0.4 mM PMSF, and 2× protease inhibitor cocktail) and incubated with Micrococcal Nuclease (MNase) (Worthington) at a final concentration of 25 U ml−1 for 20 min. ..

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  • 86
    Worthington Biochemical staphylococcal
    Staphylococcal, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/staphylococcal/product/Worthington Biochemical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    staphylococcal - by Bioz Stars, 2020-11
    86/100 stars
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