isradipine  (Alomone Labs)


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    Structured Review

    Alomone Labs isradipine
    ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents <t>(isradipine-sensitive</t> component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P
    Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isradipine/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    isradipine - by Bioz Stars, 2022-12
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    Images

    1) Product Images from "Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion"

    Article Title: Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion

    Journal: Communications Biology

    doi: 10.1038/s42003-022-03170-w

    ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents (isradipine-sensitive component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P
    Figure Legend Snippet: ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents (isradipine-sensitive component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P

    Techniques Used: Blocking Assay

    2) Product Images from "Lamellar cells in Pacinian and Meissner corpuscles are touch sensors"

    Article Title: Lamellar cells in Pacinian and Meissner corpuscles are touch sensors

    Journal: bioRxiv

    doi: 10.1101/2020.08.24.265231

    Pharmacological profile of Meissner lamellar cell firing. Quantification of the number of action potentials in response to current injection in the presence of indicated pharmacological agents: 10 µM Felodipine, a mix of 10 µM Nimodipine and 5 µM Isradipine, 10 µM Nifedipine, Agatoxin mix (1 µM ω-Agatoxin IVA and 1 µM ω-Agatoxin TK), Conotoxin mix (5 µM ω-Conotoxin CnVIIA, 10 nM ω-Conotoxin CVIB, 10 nM ω-Conotoxin CVIE, 1 µM ω-Conotoxin MVIIC and 1 µM ω-Conotoxin MVIID), 1 µM SNX-482, 5 µM Mibefradil, 200 nM Kurtoxin. Thin lines represent individual cells, thick lines connect means ± s.e.m. Data were obtained from at least two independent experiments.
    Figure Legend Snippet: Pharmacological profile of Meissner lamellar cell firing. Quantification of the number of action potentials in response to current injection in the presence of indicated pharmacological agents: 10 µM Felodipine, a mix of 10 µM Nimodipine and 5 µM Isradipine, 10 µM Nifedipine, Agatoxin mix (1 µM ω-Agatoxin IVA and 1 µM ω-Agatoxin TK), Conotoxin mix (5 µM ω-Conotoxin CnVIIA, 10 nM ω-Conotoxin CVIB, 10 nM ω-Conotoxin CVIE, 1 µM ω-Conotoxin MVIIC and 1 µM ω-Conotoxin MVIID), 1 µM SNX-482, 5 µM Mibefradil, 200 nM Kurtoxin. Thin lines represent individual cells, thick lines connect means ± s.e.m. Data were obtained from at least two independent experiments.

    Techniques Used: Injection

    3) Product Images from "Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels"

    Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

    Journal: Molecular psychiatry

    doi: 10.1038/s41380-019-0513-2

    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
    Figure Legend Snippet: LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p

    Techniques Used: Conditioned Place Preference, SPR Assay, Mouse Assay

    Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p
    Figure Legend Snippet: Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p

    Techniques Used: Mouse Assay, Injection, Expressing, Imaging, SPR Assay, Blocking Assay

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    Alomone Labs isradipine
    ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents <t>(isradipine-sensitive</t> component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P
    Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isradipine/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Alomone Labs naringenin
    ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents <t>(isradipine-sensitive</t> component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P
    Naringenin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/naringenin/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Alomone Labs cyppa
    SK3–Orai1 interplay in LNCaP cells: Inset table and chemical structures represent the used agonist and antagonist of SK and Orai1 channels. ( A ) Cell viability of LNCaP cells after 24, 48, 72, and 96 h upon the treatment with SK3 channel agonist 10 μM <t>Cyppa,</t> 5 μM NS309, and antagonist 30 μM <t>NS8593,</t> 10 mM 4-AP detected via MTS assay; ( B ) Block diagram represents the cell proliferation of LNCaP cells after 96 h upon conditions described in ( A ). The Mann–Whitney test was used for statistical comparison considering differences statistically significant at p
    Cyppa, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    cyppa - by Bioz Stars, 2022-12
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    ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents (isradipine-sensitive component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P

    Journal: Communications Biology

    Article Title: Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion

    doi: 10.1038/s42003-022-03170-w

    Figure Lengend Snippet: ACC1 is necessary for the maintenance of voltage-gated calcium currents in alpha cells. Electrophysiological recordings of Ca 2+ currents triggered by a series of 20 ms depolarising pulses (from −70 to +40 mV with a 10 mV step increment) from a holding potential of −70 mV in control (black trace/squares) and gluACC1KO (red trace/circles) alpha cells within intact islets. Current–voltage relationships were fitted with a Boltzmann function. a , b Representative Ca 2+ current trace ( a ) and summary of the amplitude of Ca 2+ currents ( b ) in the presence of 0.1 µg/ml tetrodotoxin to block Na + channels. c Summary of the amplitude of P/Q-type Ca 2+ currents (ω-agatoxin VIA-sensitive component). d Summary of the amplitude of L-type Ca 2+ currents (isradipine-sensitive component). Data from n = 4 independent islet preparations analysed by t -test. All data presented as mean ± SEM. Significance threshold: * P

    Article Snippet: TOFA (5-(tetradecyloxy)-2-furoic acid ; ab141578) and Tolbutamide were purchased from Abcam; Noradrenaline from Tocris Bioscience (Bristol, UK); 2-Bromopalmitate from Sigma-Aldrich; Tetrodotoxin, ω-agatoxin and isradipine from Alomone Labs (Jerusalem, Israel).

    Techniques: Blocking Assay

    SK3–Orai1 interplay in LNCaP cells: Inset table and chemical structures represent the used agonist and antagonist of SK and Orai1 channels. ( A ) Cell viability of LNCaP cells after 24, 48, 72, and 96 h upon the treatment with SK3 channel agonist 10 μM Cyppa, 5 μM NS309, and antagonist 30 μM NS8593, 10 mM 4-AP detected via MTS assay; ( B ) Block diagram represents the cell proliferation of LNCaP cells after 96 h upon conditions described in ( A ). The Mann–Whitney test was used for statistical comparison considering differences statistically significant at p

    Journal: Cancers

    Article Title: Orai1 Boosts SK3 Channel Activation

    doi: 10.3390/cancers13246357

    Figure Lengend Snippet: SK3–Orai1 interplay in LNCaP cells: Inset table and chemical structures represent the used agonist and antagonist of SK and Orai1 channels. ( A ) Cell viability of LNCaP cells after 24, 48, 72, and 96 h upon the treatment with SK3 channel agonist 10 μM Cyppa, 5 μM NS309, and antagonist 30 μM NS8593, 10 mM 4-AP detected via MTS assay; ( B ) Block diagram represents the cell proliferation of LNCaP cells after 96 h upon conditions described in ( A ). The Mann–Whitney test was used for statistical comparison considering differences statistically significant at p

    Article Snippet: Inhibitors (NS8593 hydrochloride Cat #: N-195, 4-AP Cat #: A-115) and activators (NS-309 Cat #: N-180, 1-EBIO Cat #: E-150, Cyppa Cat #: C-110) were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: MTS Assay, Blocking Assay, MANN-WHITNEY