wrw4  (Alomone Labs)


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    Structured Review

    Alomone Labs wrw4
    PSMα1-induced neutrophil necroptosis was blocked by <t>WRW4</t> or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Wrw4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wrw4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wrw4 - by Bioz Stars, 2021-12
    94/100 stars

    Images

    1) Product Images from "Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system"

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0398-z

    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Figure Legend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Techniques Used: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system"

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0398-z

    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Figure Legend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Techniques Used: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay

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    Alomone Labs wrw4
    PSMα1-induced neutrophil necroptosis was blocked by <t>WRW4</t> or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P
    Wrw4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wrw4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wrw4 - by Bioz Stars, 2021-12
    94/100 stars
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    PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Journal: Cell Death & Disease

    Article Title: Inhibiting PSMα-induced neutrophil necroptosis protects mice with MRSA pneumonia by blocking the agr system

    doi: 10.1038/s41419-018-0398-z

    Figure Lengend Snippet: PSMα1-induced neutrophil necroptosis was blocked by WRW4 or anti-TNFα. a, c Neutrophil were treated with PBS or TNFα neutralizing antibody at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6) and the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3). b, d, e Neutrophil were treated with PBS or WRW4 at indicated concentrations for 1 h prior to the incubation with 1 μM PSMα1 for 1 h. The release of LDH was detected ( n = 6), the level of pMLKL, MLKL and β-actin in neutrophil were detected by western blot ( n = 3), and the level of TNFα was detected by ELISA assay ( n = 8). Data were shown as mean ± SE (a, b, e). * P

    Article Snippet: Neutrophil cells were respectively stimulated with PSM peptide for 1 h at 37 °C and then centrifugated (1000 rpm, 10 min) after pretreated with or without NSA, Nec, WRW4, anti-TNFα for 1 h. The neutrophil cell culture supernatant is used to detect the release of LDH.

    Techniques: Incubation, Western Blot, Enzyme-linked Immunosorbent Assay