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human tau 2n4r  (StressMarq)


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    Structured Review

    StressMarq human tau 2n4r
    A) IPSC-derived neurons were transduced with Halo-tagged <t>human</t> <t>tau</t> (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .
    Human Tau 2n4r, supplied by StressMarq, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SPR-497/bio_rxiv__2025__03__02__641073-79-5-16?v=StressMarq
    Average 94 stars, based on 1 article reviews
    human tau 2n4r - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA"

    Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA

    Journal: bioRxiv

    doi: 10.1101/2025.03.02.641073

    A) IPSC-derived neurons were transduced with Halo-tagged human tau (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .
    Figure Legend Snippet: A) IPSC-derived neurons were transduced with Halo-tagged human tau (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .

    Techniques Used: Derivative Assay, Transduction, Incubation, Labeling



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    human tau 2n4r  (StressMarq)
    94
    StressMarq human tau 2n4r
    A) IPSC-derived neurons were transduced with Halo-tagged <t>human</t> <t>tau</t> (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .
    Human Tau 2n4r, supplied by StressMarq, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SPR-497/bio_rxiv__2025__03__02__641073-79-5-16?v=StressMarq
    Average 94 stars, based on 1 article reviews
    human tau 2n4r - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    A) IPSC-derived neurons were transduced with Halo-tagged human tau (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .

    Journal: bioRxiv

    Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA

    doi: 10.1101/2025.03.02.641073

    Figure Lengend Snippet: A) IPSC-derived neurons were transduced with Halo-tagged human tau (Halo-tau) and LAMP1- BFP (red). Transduced neurons were then incubated with no aggregates, Aβ42o alone (magenta), tauPFFs alone (cyan), or both Aβ42o and tauPFFs together for 18 hours (overnight). Excess aggregates were washed off following loading, and Halo-tau was labeled using JaneliaFluor- labeled Halo-tag ligand (green). Neurons were then imaged live directly after this labeling (0 hrs), or imaged at 6 hours, 24 hours, 48 hours, 5 days, and 7 days after Halo-tag labeling. Colocalization between labeled Halo-tau and LAMP1-mCh was quantitated (white pixels). B) The mean percentage of Halo-tau colocalized with LAMP1 was analyzed from three replicate experiments (n=3), each containing at least 6 individual neuron cell body images at each time point for each treatment. Significant differences between each condition within a time point were analyzed using a two-way ANOVA with Sidak’s test. Data are presented as mean ± SEM. * p<0.05. Scale bar = 5um .

    Article Snippet: PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol.

    Techniques: Derivative Assay, Transduction, Incubation, Labeling