egcg stabilized α synuclein oligomers (StressMarq)

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StressMarq egcg stabilized α synuclein oligomers

Microglial inflammatory gene expression induced <t>by</t> <t>α-synuclein</t> polymorphs. A Electron microscopy analysis of α-synuclein EO, KSO, DO, PFF, sPFF, and Fib. Scale bars 200 nm (Upper panel) and 20 nm (Lower panel). B Experimental scheme. iPSC-differentiated iMG were exposed to control, α-synuclein MO, EO, KSO, DO, PFF, sPFF, or Fib for 4 h. C–J iMG expressions of TNF ( C ), IL-1β ( D ), IL-6 ( E ), IL-10 ( F ), CCL2 ( G ), CCL3 ( H ), CCL4 ( I ), and CXCL1 ( J ) were determined by quantitative polymerase chain reaction (PCR). Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test ( n = 4 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Egcg Stabilized α Synuclein Oligomers, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/SPR-469/Alpha+Synuclein+Oligomers/pmc12797977-14-12-33
Average 93 stars, based on 2 article reviews

egcg stabilized α synuclein oligomers - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Differential microglial responses to structurally distinct alpha-synuclein polymorphs"

Article Title: Differential microglial responses to structurally distinct alpha-synuclein polymorphs

Journal: Molecular Brain

doi: 10.1186/s13041-025-01256-0

Microglial inflammatory gene expression induced by α-synuclein polymorphs. A Electron microscopy analysis of α-synuclein EO, KSO, DO, PFF, sPFF, and Fib. Scale bars 200 nm (Upper panel) and 20 nm (Lower panel). B Experimental scheme. iPSC-differentiated iMG were exposed to control, α-synuclein MO, EO, KSO, DO, PFF, sPFF, or Fib for 4 h. C–J iMG expressions of TNF ( C ), IL-1β ( D ), IL-6 ( E ), IL-10 ( F ), CCL2 ( G ), CCL3 ( H ), CCL4 ( I ), and CXCL1 ( J ) were determined by quantitative polymerase chain reaction (PCR). Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test ( n = 4 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Figure Legend Snippet: Microglial inflammatory gene expression induced by α-synuclein polymorphs. A Electron microscopy analysis of α-synuclein EO, KSO, DO, PFF, sPFF, and Fib. Scale bars 200 nm (Upper panel) and 20 nm (Lower panel). B Experimental scheme. iPSC-differentiated iMG were exposed to control, α-synuclein MO, EO, KSO, DO, PFF, sPFF, or Fib for 4 h. C–J iMG expressions of TNF ( C ), IL-1β ( D ), IL-6 ( E ), IL-10 ( F ), CCL2 ( G ), CCL3 ( H ), CCL4 ( I ), and CXCL1 ( J ) were determined by quantitative polymerase chain reaction (PCR). Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test ( n = 4 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Gene Expression, Electron Microscopy, Control, Real-time Polymerase Chain Reaction, Comparison

Transcriptomic analysis of microglia exposed to α-synuclein polymorphs. iPSC-differentiated iMicroglia were exposed to control, α-synuclein MO, EO, KSO, DO, PFF, sPFF, or Fib for 4 h ( n = 4 per group). Total RNA extracted from iMG was analyzed by RNA sequencing. A Heatmap of the differentially expressed genes in the group of comparisons. Red and blue represent down- and up-regulation (Cut-off criteria: FDR < 0.05 and the absolute value of log 2 FC ≥ 0.58). B–K GO:BP enrichment analysis (Fisher’s exact test p -value with FDR correction < 0.05). Enriched GO:BP pathways in iMG expose to α-synuclein aggregates, response to cytokine ( B ), cytokine-mediated signaling pathway ( C ), response to chemokine ( D ), inflammatory response ( E ), regulation of response to stimulus ( F ), cellular response to chemical stimulus ( g ), cell surface receptor signaling pathway ( H ), defense response ( I ), response to tumor necrosis factor ( J ), and leukocyte chemotaxis ( K ). The y -axis displays treatments, and the x -axis shows the -log 10 p -value. L Radar plot for p-values of enriched KEGG immune pathway of common up-regulated genes among the group of comparisons

Figure Legend Snippet: Transcriptomic analysis of microglia exposed to α-synuclein polymorphs. iPSC-differentiated iMicroglia were exposed to control, α-synuclein MO, EO, KSO, DO, PFF, sPFF, or Fib for 4 h ( n = 4 per group). Total RNA extracted from iMG was analyzed by RNA sequencing. A Heatmap of the differentially expressed genes in the group of comparisons. Red and blue represent down- and up-regulation (Cut-off criteria: FDR < 0.05 and the absolute value of log 2 FC ≥ 0.58). B–K GO:BP enrichment analysis (Fisher’s exact test p -value with FDR correction < 0.05). Enriched GO:BP pathways in iMG expose to α-synuclein aggregates, response to cytokine ( B ), cytokine-mediated signaling pathway ( C ), response to chemokine ( D ), inflammatory response ( E ), regulation of response to stimulus ( F ), cellular response to chemical stimulus ( g ), cell surface receptor signaling pathway ( H ), defense response ( I ), response to tumor necrosis factor ( J ), and leukocyte chemotaxis ( K ). The y -axis displays treatments, and the x -axis shows the -log 10 p -value. L Radar plot for p-values of enriched KEGG immune pathway of common up-regulated genes among the group of comparisons

Techniques Used: Control, RNA Sequencing, Cell Surface Receptor Assay, Chemotaxis Assay

TLR agonist ability of α-synuclein oligomers. A Schematic illustration of experiment. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein oligomers and α-synuclein MO. B – H After 24 h of incubation, the activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Figure Legend Snippet: TLR agonist ability of α-synuclein oligomers. A Schematic illustration of experiment. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein oligomers and α-synuclein MO. B – H After 24 h of incubation, the activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Incubation, Activity Assay, Reporter Assay, Comparison

TLR induction ability of α-synuclein fibrillar species. A Experimental scheme. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein PFF and α-synuclein sPFF. B–H The activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay after a 24-h incubation ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01

Figure Legend Snippet: TLR induction ability of α-synuclein fibrillar species. A Experimental scheme. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein PFF and α-synuclein sPFF. B–H The activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay after a 24-h incubation ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01

Techniques Used: Activity Assay, Reporter Assay, Incubation, Comparison

Inhibition of TLR2 reduces α-synuclein polymorphs-induced inflammatory gene expression in iMicroglia. A Experimental scheme. iMG were pretreated with either control IgG (1 μg/mL) or anti-TLR2 antibody (T2.5, 1 μg/ML) for 30 min, followed by treatment with control, α-synuclein KSO, or Fib for 4 h. B–E Expression levels of TNF ( B ), IL-6 ( C ), CCL2 ( D ), and CCL4 ( E ) in iMG were assessed by quantitative PCR. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Figure Legend Snippet: Inhibition of TLR2 reduces α-synuclein polymorphs-induced inflammatory gene expression in iMicroglia. A Experimental scheme. iMG were pretreated with either control IgG (1 μg/mL) or anti-TLR2 antibody (T2.5, 1 μg/ML) for 30 min, followed by treatment with control, α-synuclein KSO, or Fib for 4 h. B–E Expression levels of TNF ( B ), IL-6 ( C ), CCL2 ( D ), and CCL4 ( E ) in iMG were assessed by quantitative PCR. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Techniques Used: Inhibition, Gene Expression, Control, Expressing, Real-time Polymerase Chain Reaction, Comparison

Recombinant:

Article Title: Differential microglial responses to structurally distinct alpha-synuclein polymorphs
Article Snippet: .. Recombinant human α-synuclein monomers (#SPR-316, MO), kinetically stable α-synuclein oligomers (#SPR-484, KSO), EGCG stabilized α-synuclein oligomers (#SPR-469, EO), dopamine stabilized α-synuclein oligomers (#SPR-466, DO), and α-synuclein preformed fibrils (#SPR-322, PFF) were purchased from StressMarq Biosciences (Victoria, Canada). .. To obtain sonicated preformed fibrils (sPFF), PFF were sonicated at room temperature using an ultrasonic homogenizer (Thermo Fisher Scientific, Waltham, MA).

Article Title: Differential microglial responses to structurally distinct alpha-synuclein polymorphs.
Article Snippet: .. AR TIC LE IN PR ES S 5 Materials and methods Preparation of -synuclein aggregates Recombinant human -synuclein monomers (#SPR-316, MO), kinetically stable -synuclein oligomers (#SPR-484, KSO), EGCG stabilized -synuclein oligomers (#SPR-469, EO), dopamine stabilized -synuclein oligomers (#SPR-466, DO), and -synuclein preformed fibrils (#SPR-322, PFF) were purchased from StressMarq Biosciences (Victoria, Canada). .. To obtain sonicated preformed fibrils (sPFF), PFF were sonicated at room temperature using an ultrasonic homogenizer (Thermo Fisher Scientific, Waltham, MA).

Article Title: The Neurotoxic Properties of α-synuclein Polymorphs
Article Snippet: The following antibodies were used for immunoblot analysis: α-syn (Cat. PA5-8543) from Invitrogen, PARP (Cat. 9542), p-mTOR (Cat. 2974), Bcl2 (Cat. 15071), Cytochrome c (Cat. 4272), and CHOP (Cat. 2895) from Cell Signaling (Beverly, MA); p62 (Cat. P0067) and β-actin (Cat. A5441) from Sigma-Aldrich; Active caspase-3 (Cat. AF835) from R&D Systems (Minneapolis, MN); LC3 (Cat. PD014) from MBL (Tokyo, Japan); IRDye ® 800CW Goat anti-Rabbit IgG (Cat. 925-32211), IRDye ® 800CW Goat anti-Mouse IgG (Cat. 925-32210), IRDye ® 680RD Goat anti-Rabbit IgG (Cat. 925-68071), and IRDye ® 680RD Goat anti-Mouse IgG (Cat. 925-68070) from LI-COR Biosciences (Lincoln, NE). .. Recombinant human α-syn monomers (#SPR-316, MO), α-syn preformed fibrils (#SPR-322, PFF), kinetically stable α-syn oligomers (#SPR-484, KSO), dopamine stabilized α-syn oligomers (#SPR-466, DO), and epigallocatechin gallate (EGCG) stabilized α-syn oligomers (#SPR-469, EO) were obtained from StressMarq Biosciences (Victoria, Canada). .. To prepare sonicated α-syn preformed fibrils (sPFF), PFF were sonicated utilizing ultrasonic homogenizer (Thermo Fisher Scientific, Waltham, MA).

Article Title: The Neurotoxic Properties of α-synuclein Polymorphs.
Article Snippet: The following antibodies were used for immunoblot analysis: α-syn (Cat. PA5-8543) from Invitrogen, PARP (Cat. 9542), p-mTOR (Cat. 2974), Bcl2 (Cat. 15071), Cytochrome c (Cat. 4272), and CHOP (Cat. 2895) from Cell Signaling (Beverly, MA); p62 (Cat. P0067) and β-actin (Cat. A5441) from Sigma-Aldrich; Active caspase-3 (Cat. AF835) from R&D Systems (Minneapolis, MN); LC3 (Cat. PD014) from MBL (Tokyo, Japan); IRDye® 800CW Goat anti-Rabbit IgG (Cat. 925- 32211), IRDye® 800CW Goat anti-Mouse IgG (Cat. 925-32210), IRDye® 680RD Goat anti-Rabbit IgG (Cat. 925-68071), and IRDye® 680RD Goat anti-Mouse IgG (Cat. 925-68070) from LICOR Biosciences (Lincoln, NE). .. Recombinant human α-syn monomers (#SPR-316, MO), α-syn preformed fibrils (#SPR-322, PFF), kinetically stable α-syn oligomers (#SPR-484, KSO), dopamine stabilized α-syn oligomers (#SPR-466, DO), and epigallocatechin gallate (EGCG) stabilized α-syn oligomers (#SPR-469, EO) were obtained from StressMarq Biosciences (Victoria, Canada). .. To prepare sonicated α-syn preformed fibrils (sPFF), PFF were sonicated utilizing ultrasonic homogenizer (Thermo Fisher Scientific, Waltham, MA).

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egcg stabilized α synuclein oligomers (StressMarq)

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