Recombinant:Article Title: Circadian timing and entrainment properties of the SCN pacemaker in the PS19 mouse model of tau pathology.
Article Snippet: Long-term imaging of Venus expression was performed using YFP (yellow fluorescent protein) acquisition settings, and time-lapse based image acquisition was performed at 1-h intervals for up to 6 weeks (for a detailed description of the imaging methods, see Wheaton et al., 2018 and Hoyt et al., 2023). .. For tau seeding experiments, after baseline acquisition, SCN explants were treated with 1.7 μg/ml or 7.0 μg/ml human recombinant Tau-441 (2N4R) P301S mutant protein pre-formed fibrils (tau PFF; Stressmarq SPR329). ..
Article Title: Circadian timing and entrainment properties of the SCN pacemaker in the PS19 mouse model of tau pathology
Article Snippet: .. For tau seeding experiments, after baseline acquisition, SCN explants were treated with 1.7 μg/ml or 7.0 μg/ml human recombinant Tau-441 (2N4R) P301S mutant protein pre-formed fibrils (tau PFF; Stressmarq SPR-329). .. Per1 -Venus expression profiling of SCN explants was performed using Fiji/ImageJ , and Biodare2 ( ; ) was used to linear detrend the Venus time-lapse data and calculate the period of cellular Venus expression (FFT-NLLS method) during acquisition.
Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA
Article Snippet: .. PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol. ..
Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Mutagenesis:Article Title: Circadian timing and entrainment properties of the SCN pacemaker in the PS19 mouse model of tau pathology.
Article Snippet: Long-term imaging of Venus expression was performed using YFP (yellow fluorescent protein) acquisition settings, and time-lapse based image acquisition was performed at 1-h intervals for up to 6 weeks (for a detailed description of the imaging methods, see Wheaton et al., 2018 and Hoyt et al., 2023). .. For tau seeding experiments, after baseline acquisition, SCN explants were treated with 1.7 μg/ml or 7.0 μg/ml human recombinant Tau-441 (2N4R) P301S mutant protein pre-formed fibrils (tau PFF; Stressmarq SPR329). ..
Article Title: Circadian timing and entrainment properties of the SCN pacemaker in the PS19 mouse model of tau pathology
Article Snippet: .. For tau seeding experiments, after baseline acquisition, SCN explants were treated with 1.7 μg/ml or 7.0 μg/ml human recombinant Tau-441 (2N4R) P301S mutant protein pre-formed fibrils (tau PFF; Stressmarq SPR-329). .. Per1 -Venus expression profiling of SCN explants was performed using Fiji/ImageJ , and Biodare2 ( ; ) was used to linear detrend the Venus time-lapse data and calculate the period of cellular Venus expression (FFT-NLLS method) during acquisition.
Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA
Article Snippet: .. PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol. ..
SPR Assay:Article Title: Facile generation of drug-like conformational antibodies specific for amyloid fibrils.
Article Snippet: Antibodies that recognize insoluble antigens, such as amyloid fibrils associated with neurodegenerative disorders, are important for research, diagnostic and therapeutic applications.. However, these types of antibodies are difficult to generate, typically require animal immunization and also commonly require humanization in the case of therapeutic applications.. Here we report a methodology for generating high-quality, fully human, conformation-specific antibodies against amyloid fibrils using a published human nonimmune library, yeast-surface display and quantitative fluorescence-activated cell sorting.
Article Title: Circadian timing and entrainment properties of the SCN pacemaker in the PS19 mouse model of tau pathology
Article Snippet: .. For tau seeding experiments, after baseline acquisition, SCN explants were treated with 1.7 μg/ml or 7.0 μg/ml human recombinant Tau-441 (2N4R) P301S mutant protein pre-formed fibrils (tau PFF; Stressmarq SPR-329). .. Per1 -Venus expression profiling of SCN explants was performed using Fiji/ImageJ , and Biodare2 ( ; ) was used to linear detrend the Venus time-lapse data and calculate the period of cellular Venus expression (FFT-NLLS method) during acquisition.
Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA
Article Snippet: .. PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol. ..
Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Sonication:Article Title: Facile generation of drug-like conformational antibodies specific for amyloid fibrils.
Article Snippet: Antibodies that recognize insoluble antigens, such as amyloid fibrils associated with neurodegenerative disorders, are important for research, diagnostic and therapeutic applications.. However, these types of antibodies are difficult to generate, typically require animal immunization and also commonly require humanization in the case of therapeutic applications.. Here we report a methodology for generating high-quality, fully human, conformation-specific antibodies against amyloid fibrils using a published human nonimmune library, yeast-surface display and quantitative fluorescence-activated cell sorting.
Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA
Article Snippet: .. PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol. ..
Membrane:Article Title: Facile generation of drug-like conformational antibodies specific for amyloid fibrils.
Article Snippet: Antibodies that recognize insoluble antigens, such as amyloid fibrils associated with neurodegenerative disorders, are important for research, diagnostic and therapeutic applications.. However, these types of antibodies are difficult to generate, typically require animal immunization and also commonly require humanization in the case of therapeutic applications.. Here we report a methodology for generating high-quality, fully human, conformation-specific antibodies against amyloid fibrils using a published human nonimmune library, yeast-surface display and quantitative fluorescence-activated cell sorting.
Generated:Article Title: Macropinocytosis of aggregated amyloid-beta and tau requires Arf6 and the RhoGTPases Rac1, Cdc42 and RhoA
Article Snippet: .. PFFs were generated from recombinant human tau (2N4R) with a P301S mutation tagged with ATTO 488 (StressMarq; SPR-329B-A488; Canada) following manufacturers sonication protocol. ..
Enzyme-linked Immunosorbent Assay:Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Staining:Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Cell Stimulation:Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Gene Knockout:Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
Counting:Article Title: Peripheral CXCR3 blockade mitigates T cell infiltration and neurodegeneration in a mouse model of tauopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant Murine IFN-beta R&D Systems Cat# 8234-MB-CF Recombinant Murine CXCL10 PeproTech Cat# 250-16 Recombinant Human P301S Tau Monomers StressMarq Biosciences Cat# SPR-327 Recombinant Human P301S Tau PFFs StressMarq Biosciences Cat# SPR-329 Percoll Sigma-Aldrich Cat# P1644 DPBS Gibco Cat# 14190144 1X HBSS Gibco Cat# 14025076 10X HBSS Gibco Cat# 14065056 RPMI Gibco Cat# 11875093 DAPI ThermoFisher Cat# 62248 TrueBlack Lipofuscin Autofluorescence Quencher Biotium Cat# 23007 ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934 Critical Commercial Assays SIMOA NF-Light Advantage Kit Quanterix Cat# 103186 Mouse IFN-gamma DuoSet ELISA Thermo Fisher Scientific Cat# 88-8314-88 Mouse CXCL10 DuoSet ELISA R&D Systems Cat# DY466 Foxp3 Transcription Factor Staining Kit Thermo Fisher Scientific / eBioscience Cat# 00-5523-00 Cell Stimulation Cocktail (PMA/Ionomycin) Thermo Fisher Scientific Cat# 00-4975-93 Deposited data scRNA-Seq datasets This paper #GSE344425 Experimental Models: Organisms/Strains Mouse: B6.Cg-MaptTt1(GDNFMAPT*P301S)1Hnd/J (PS19) Provided by original developer (Yoshiyama et al., 2007) / Jackson Laboratory RRID:IMSR_JAX:008169 Mouse: B6.129P2-Apoetm3(APOE*E4)Mae N8 (Human APOE4 KI) Jackson Laboratory Strain #027894; RRID:IMSR_TAC:9065 Mouse: B6.129S4-Cxcl10tm1Adl/J (Cxcl10 KO) Jackson Laboratory Strain #006087; RRID:IMSR_JAX:006087 Mouse: B6.129P2-Cxcr3tm1Mwm/J (Cxcr3 KO) Jackson Laboratory Strain #005796; RRID:IMSR_JAX:005796 Software and Algorithms ANY-maze Video Tracking System Stoelting Co. RRID:SCR_014289 FlowJo v10.10 FlowJo, LLC RRID:SCR_008520 Seurat R Package Satija Lab RRID:SCR_007322 Harmony R Package Korsunsky et al. https://github.com/immunogenomics/ harmony Cell Ranger V7.2.0 10X Genomics RRID:SCR_017344 Fiji / ImageJ NIH RRID:SCR_002285 FreezeFrame Actimetrics RRID:SCR_014429 Other AIN-76A Rodent Diet Research Diets Inc. Cat# D10001 PLX3397 Formulated Diet (400 mg/kg in AIN-76A) Research Diets Inc. Cat# D13110610 123count eBeads Counting Beads Thermo Fisher Scientific Cat# 01123442 30-gauge RN 1.5-inch 60◦ Needles Hamilton Instruments Cat# 7803-07 1701RN gastight syringe Hamilton Instruments Cat# 7653-01 ll OPEN ACCESSArticle Neuron 115, 1–15.e1–e6, February 17, 2027 e2 ..
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