trueview  (Vector Laboratories)


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    Name:
    Vector TrueVIEW Autofluorescence Quenching Kit
    Description:
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    Catalog Number:
    SP-8400
    Price:
    None
    Category:
    Protein chemifluorescent detection reagents or kits or substrates
    Size:
    15 ml
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    Structured Review

    Vector Laboratories trueview
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    https://www.bioz.com/result/trueview/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trueview - by Bioz Stars, 2021-05
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: Immunostaining was performed on 3 μm-thick sections using antibodies against GFP (ICL lab), Alexa Fluor 647-directly labeled B220 (eBioscience) and Lectin PNA Alexa Fluor 488 Conjugate (ThermoFisher). .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Plasmid Preparation:

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: Immunostaining was performed on 3 μm-thick sections using antibodies against GFP (ICL lab), Alexa Fluor 647-directly labeled B220 (eBioscience) and Lectin PNA Alexa Fluor 488 Conjugate (ThermoFisher). .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Article Title: Presence of Clock genes in equine full-term placenta
    Article Snippet: .. Sections were rinsed three times with 1× PBS, then quenched for 5 min at room temperature with hydrogen peroxide (H2 O2) and Vector TrueView Autofluorescence Quenching Kit. ..

    Article Title: Histone deacetylases 1 and 2 silence cryptic transcription to promote mitochondrial function during cardiogenesis
    Article Snippet: Eosin Y and Harris modified hematoxylin were obtained from Fisher, while X-gal was purchased from 5-Prime. .. Vectashield permanent mounting medium, Vectashield fluorescent mounting medium, Vectashield Elite ABC reagent kit, Vector TrueVIEW Autofluorescence Quenching Kit, and DAB Peroxidase Substrate kit were purchased from Vector Labs. .. ProLong Glass Antifade Mounting Medium was purchased from Thermo Fisher Scientific.

    Article Title: Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
    Article Snippet: Then, sections were washed in PBS for 3 × 5 min. After the last wash step secondary antibodies diluted in blocking buffer were added for additional 30 min incubation, RT. .. A second wash with PBS for 3 × 5 min, RT, was conducted prior to the use of a second auto fluorescence quenching kit (Vector TrueVIEW autofluorescence quenching kit, Vector Laboratories, # SP-8400). .. Finally, sections were washed in PBS for 5 min, incubated with DAPI (conc.

    Confocal Microscopy:

    Article Title: Novel diagnostic and therapeutic techniques reveal changed metabolic profiles in recurrent focal segmental glomerulosclerosis
    Article Snippet: Nuclei of cells were stained with DAPI in distilled water (0.2 µg/ml) for 5 min followed by rinsing in Tris buffer. .. Finally, sections were covered with TrueView mounting kit (Vector laboratories) and analyzed using laser scanning confocal microscopy (LSM Zeiss 710 and Zen software, Zeiss GmbH, Jena, Germany) and Image-J software. .. Negative controls for immunostaining included either deletion or substitution of the primary antibody with equivalent concentrations of an irrelevant murine monoclonal antibody or pre-immune rabbit IgG.

    Software:

    Article Title: Novel diagnostic and therapeutic techniques reveal changed metabolic profiles in recurrent focal segmental glomerulosclerosis
    Article Snippet: Nuclei of cells were stained with DAPI in distilled water (0.2 µg/ml) for 5 min followed by rinsing in Tris buffer. .. Finally, sections were covered with TrueView mounting kit (Vector laboratories) and analyzed using laser scanning confocal microscopy (LSM Zeiss 710 and Zen software, Zeiss GmbH, Jena, Germany) and Image-J software. .. Negative controls for immunostaining included either deletion or substitution of the primary antibody with equivalent concentrations of an irrelevant murine monoclonal antibody or pre-immune rabbit IgG.

    Fluorescence:

    Article Title: Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background
    Article Snippet: Then, sections were washed in PBS for 3 × 5 min. After the last wash step secondary antibodies diluted in blocking buffer were added for additional 30 min incubation, RT. .. A second wash with PBS for 3 × 5 min, RT, was conducted prior to the use of a second auto fluorescence quenching kit (Vector TrueVIEW autofluorescence quenching kit, Vector Laboratories, # SP-8400). .. Finally, sections were washed in PBS for 5 min, incubated with DAPI (conc.

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  • 97
    Vector Laboratories detection kits
    Detection Kits, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/detection kits/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    detection kits - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

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