auto fluorescence  (Vector Laboratories)


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    Name:
    Vector TrueVIEW Autofluorescence Quenching Kit
    Description:
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    Catalog Number:
    sp-8400
    Price:
    None
    Size:
    15 ml
    Category:
    Protein chemifluorescent detection reagents or kits or substrates
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    Structured Review

    Vector Laboratories auto fluorescence
    The Vector TrueVIEW Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non lipofuscin sources and dramatically improve signal to noise ratio It removes unwanted autofluorescence in tissue sections due to aldehyde fixation red blood cells and structural elements such as collagen and elastin The quenching action of the kit reagents provides a clear unambiguous “true view localization of the target antigen
    https://www.bioz.com/result/auto fluorescence/product/Vector Laboratories
    Average 91 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    auto fluorescence - by Bioz Stars, 2021-02
    91/100 stars

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    Related Articles

    Staining:

    Article Title: CD4+ Resident Memory T Cells Mediate Long-Term Local Skin Immune Memory of Contact Hypersensitivity in BALB/c Mice
    Article Snippet: .. Regarding γδTCR IF, the sections were coverslipped with VECTASHIELD HardSet Antifade Mounting Medium with DAPI (Vector Laboratories), while for other IF, they were treated with the TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories), stained with DAPI (Dojindo Laboratories, Kumamoto, Japan), and coverslipped with VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories). .. All images were taken using the microscope BX-53 with appropriate mirror units and the digital camera DP73 and then analyzed with cellSens software (Olympus).

    Incubation:

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Article Title: No Effect of Diet-Induced Mild Hyperhomocysteinemia on Vascular Methylating Capacity, Atherosclerosis Progression, and Specific Histone Methylation
    Article Snippet: .. After washing, arrays were incubated with secondary antibodies in blocking buffer for ≥ 1 h at RT, washed, treated with Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA, USA), and mounted under coverslips in VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories). .. The antibodies used were: rabbit anti-histone H3K27me3 pAb (1:200, Cat.# A-4039, Epigentek, Farmingdale, NY, USA, Cat.# A-4039), mouse anti-histone H3 mAb 1B1-B2 (1:200, Cat.#819404, BioLegend, San Diego, CA, USA), Alexa Fluor 555 goat anti-rabbit IgG (1:250, Cat.# A-21428 Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488 goat anti-mouse IgG3 (1:250, Cat.# A-21151, Invitrogen, Carlsbad, CA, USA).

    Article Title: Calretinin and calbindin architecture of the midline thalamus associated with prefrontal-hippocampal circuitry
    Article Snippet: .. Post incubation, sections underwent PB washes and then, sections were treated with Vector TrueVIEW Autofluorescence kit (SP-8400; Vector Labs, CA; 3 cases) for 2 minutes at room temperature to remove any background from aldehydes. .. After this step, tissue was washed 3 times with PB and subsequently, mounted in gelatin coated slides and coverslipped with VectaShield mounting medium with DAPI.

    Plasmid Preparation:

    Article Title: Histone deacetylases 1 and 2 silence cryptic transcription to promote mitochondrial function during cardiogenesis
    Article Snippet: .. Vectashield permanent mounting medium, Vectashield fluorescent mounting medium, Vectashield Elite ABC reagent kit, Vector TrueVIEW Autofluorescence Quenching Kit, and DAB Peroxidase Substrate kit were purchased from Vector Labs. .. ProLong Glass Antifade Mounting Medium was purchased from Thermo Fisher Scientific.

    Article Title: Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses
    Article Snippet: .. Immunostained slides were incubated with the Vector® TrueVIEW Autofluorescence Quenching Kit to remove autofluorescence signal (Vector Laboratories). .. Stained sections were scanned using a Panoramic Digital Slide Scanner 250 FLASH II (3DHISTECH) at 200 x magnification.

    Article Title: Differential localization of Hessian fly candidate effectors in resistant and susceptible wheat plants. Differential localization of Hessian fly candidate effectors in resistant and susceptible wheat plants
    Article Snippet: .. Once the slides became ready for mounting, autofluorescence background from wheat tissues was suppressed by incubating the sectioned slides for 5 minutes with quench reagents from a Vector® TrueVIEW® Autofluorescence Quenching Kit (Vector laboratories). .. 5.7 Confocal microscopy and image analysesConfocal microscopy was performed with Carl Zeiss 700, an inverted microscope outfitted.

    Article Title: No Effect of Diet-Induced Mild Hyperhomocysteinemia on Vascular Methylating Capacity, Atherosclerosis Progression, and Specific Histone Methylation
    Article Snippet: .. After washing, arrays were incubated with secondary antibodies in blocking buffer for ≥ 1 h at RT, washed, treated with Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA, USA), and mounted under coverslips in VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories). .. The antibodies used were: rabbit anti-histone H3K27me3 pAb (1:200, Cat.# A-4039, Epigentek, Farmingdale, NY, USA, Cat.# A-4039), mouse anti-histone H3 mAb 1B1-B2 (1:200, Cat.#819404, BioLegend, San Diego, CA, USA), Alexa Fluor 555 goat anti-rabbit IgG (1:250, Cat.# A-21428 Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488 goat anti-mouse IgG3 (1:250, Cat.# A-21151, Invitrogen, Carlsbad, CA, USA).

    Article Title: Calretinin and calbindin architecture of the midline thalamus associated with prefrontal-hippocampal circuitry
    Article Snippet: .. Post incubation, sections underwent PB washes and then, sections were treated with Vector TrueVIEW Autofluorescence kit (SP-8400; Vector Labs, CA; 3 cases) for 2 minutes at room temperature to remove any background from aldehydes. .. After this step, tissue was washed 3 times with PB and subsequently, mounted in gelatin coated slides and coverslipped with VectaShield mounting medium with DAPI.

    Blocking Assay:

    Article Title: No Effect of Diet-Induced Mild Hyperhomocysteinemia on Vascular Methylating Capacity, Atherosclerosis Progression, and Specific Histone Methylation
    Article Snippet: .. After washing, arrays were incubated with secondary antibodies in blocking buffer for ≥ 1 h at RT, washed, treated with Vector TrueVIEW Autofluorescence Quenching Kit (Vector Laboratories, Burlingame, CA, USA), and mounted under coverslips in VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories). .. The antibodies used were: rabbit anti-histone H3K27me3 pAb (1:200, Cat.# A-4039, Epigentek, Farmingdale, NY, USA, Cat.# A-4039), mouse anti-histone H3 mAb 1B1-B2 (1:200, Cat.#819404, BioLegend, San Diego, CA, USA), Alexa Fluor 555 goat anti-rabbit IgG (1:250, Cat.# A-21428 Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488 goat anti-mouse IgG3 (1:250, Cat.# A-21151, Invitrogen, Carlsbad, CA, USA).

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  • 99
    Vector Laboratories vector trueview autofluorescence quenching kit
    Vector Trueview Autofluorescence Quenching Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector trueview autofluorescence quenching kit/product/Vector Laboratories
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    vector trueview autofluorescence quenching kit - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

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