cdna synthesis  (Solis BioDyne)


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    Name:
    SOLIScript RT cDNA synthesis MIX
    Description:
    Novel in silico engineered thermostable reverse transcriptase with reduced RNase H activity SOLIScript RT is extremely stable and will remain fully active for up to one month at room temperature
    Catalog Number:
    06-37-00100
    Price:
    None
    Category:
    cDNA synthesis
    Applications:
    cDNA synthesis, first strand cDNA synthesis, reverse transcription, gene expression analysis
    Size:
    100 rxn | 20 μl rxn
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    Structured Review

    Solis BioDyne cdna synthesis
    SOLIScript RT cDNA synthesis MIX
    Novel in silico engineered thermostable reverse transcriptase with reduced RNase H activity SOLIScript RT is extremely stable and will remain fully active for up to one month at room temperature
    https://www.bioz.com/result/cdna synthesis/product/Solis BioDyne
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Involvement of histamine H1 and H2 receptor inverse agonists in receptor's crossregulation.
    Article Snippet: For the first-strand cDNA synthesis, 2 μg of total RNA was reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (AB) with random primers.

    Purification:

    Article Title: Microarray Analysis Reveals Increased Transcriptional Repression and Reduced Metabolic Activity but Not Major Changes in the Core Apoptotic Machinery during Maturation of Sympathetic Neurons
    Article Snippet: .. RNA clean-up was done with the same RNA purification kit. cDNA synthesis was performed in 20 μl reaction containing 4 μl of 5x RT buffer (Solis Biodyne, Tartu, Estonia), 2.5 μl of 2.5 mM mix of 4 dNTPs (Solis BioDyne), 1 μl of Oligo (dT)18 primer (Thermo Fisher Scientific, Rockford, IL, USA), 0.2 μl of Random Hexamer Primer (Thermo Fisher Scientific), 0.5 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific), 0.5 μl of 200 U/μl MMLV Revertase RNase H minus (Solis BioDyne) and 2 μg of RNA in 11.3 μl water. cDNA was synthesized at 37°C for 90 min and kept thereafter for 5 min at 95°C to inactivate the polymerase. ..

    Random Hexamer Labeling:

    Article Title: Microarray Analysis Reveals Increased Transcriptional Repression and Reduced Metabolic Activity but Not Major Changes in the Core Apoptotic Machinery during Maturation of Sympathetic Neurons
    Article Snippet: .. RNA clean-up was done with the same RNA purification kit. cDNA synthesis was performed in 20 μl reaction containing 4 μl of 5x RT buffer (Solis Biodyne, Tartu, Estonia), 2.5 μl of 2.5 mM mix of 4 dNTPs (Solis BioDyne), 1 μl of Oligo (dT)18 primer (Thermo Fisher Scientific, Rockford, IL, USA), 0.2 μl of Random Hexamer Primer (Thermo Fisher Scientific), 0.5 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific), 0.5 μl of 200 U/μl MMLV Revertase RNase H minus (Solis BioDyne) and 2 μg of RNA in 11.3 μl water. cDNA was synthesized at 37°C for 90 min and kept thereafter for 5 min at 95°C to inactivate the polymerase. ..

    Synthesized:

    Article Title: Microarray Analysis Reveals Increased Transcriptional Repression and Reduced Metabolic Activity but Not Major Changes in the Core Apoptotic Machinery during Maturation of Sympathetic Neurons
    Article Snippet: .. RNA clean-up was done with the same RNA purification kit. cDNA synthesis was performed in 20 μl reaction containing 4 μl of 5x RT buffer (Solis Biodyne, Tartu, Estonia), 2.5 μl of 2.5 mM mix of 4 dNTPs (Solis BioDyne), 1 μl of Oligo (dT)18 primer (Thermo Fisher Scientific, Rockford, IL, USA), 0.2 μl of Random Hexamer Primer (Thermo Fisher Scientific), 0.5 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific), 0.5 μl of 200 U/μl MMLV Revertase RNase H minus (Solis BioDyne) and 2 μg of RNA in 11.3 μl water. cDNA was synthesized at 37°C for 90 min and kept thereafter for 5 min at 95°C to inactivate the polymerase. ..

    Isolation:

    Article Title: All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice
    Article Snippet: .. Total RNA was isolated with the RNeasy Lipid Tissue Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol. cDNA synthesis was performed using SOLIScript RT Kit (Solis BioDyne, Tartu, Estonia), following the manufacturer’s instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Association of adenylate cyclase-associated protein 1 with coronary artery disease.
    Article Snippet: .. Adenylate cyclase-associated protein 1 (CAP1) is a recently identified receptor for human resistin.. As resistin has been related to CAD development and progression and CAP1 has never been evaluated in CAD, the aim of this study was to determine its peripheral blood mononuclear cells (PBMCs) mRNA in patients with CAD, and resistin plasma concentration, PBMCs resistin and CD36 mRNA, considering resistiǹs ability to stimulate CD36 expression in vitro. ..

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  • 95
    Solis BioDyne cdna synthesis
    Cdna Synthesis, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cdna synthesis/product/Solis BioDyne
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cdna synthesis - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

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