Journal: JVS-Vascular Science
Article Title: Zinc deficiency impairs ischemia-induced angiogenesis
doi: 10.1016/j.jvssci.2021.09.023
Figure Lengend Snippet: Zinc deficiency ( ZD ) increased the rate of oxidative damage in ischemic muscles. A, Production of reactive oxygen species (ROS) was evaluated by immunostaining with dihydroethidium (DHE) on postoperative day 28 (×400 for each field; red ). Serum levels of the derivatives of reactive oxygen metabolites (d-ROMs; B ) and nitrotyrosine (C) in the ZD mice or control wild-type (WT) mice after hindlimb surgery (n = 8 in each group). D, Messenger RNA (mRNA) levels of Nox2, p22 phox , p47 phox , and p67 phox in the ischemic muscle in the ZD mice or control WT mice on postoperative day 28. mRNA levels were measured using the real-time polymerase chain reaction (PCR) method (n = 8 in each group). All results were normalized to glyceraldehyde 3-phosphate dehydrogenase. ∗ P < .01 vs control.
Article Snippet: The serum nitrotyrosine levels were measured using a nitrotyrosine enzyme-linked immunosorbent assay kit (StressMarq Biosciences Inc, Victoria, British Columbia, Canada) according to the manufacturer's instruction.
Techniques: Immunostaining, Real-time Polymerase Chain Reaction