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alpha b crystallin elisa kit  (StressMarq)


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    StressMarq alpha b crystallin elisa kit
    Alpha B Crystallin Elisa Kit, supplied by StressMarq, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SKT-123/custom%40skt-123%4040809297?v=StressMarq
    Average 91 stars, based on 6 article reviews
    alpha b crystallin elisa kit - by Bioz Stars, 2026-07
    91/100 stars

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    Intracellular αB-crystallin protein level was maintained regardless of IL-1β-induced synthesis suppression in retinal Müller cells. ( A, B ) Dose-dependency ( A ) and time-dependency ( B ) of CRYAB mRNA levels under IL-1β treatment were analyzed by reverse transcription qPCR analyses. ( C, D ) Extracellular αB-crystallin protein level in 2 mL culture medium ( C ) and intracellular αB-crystallin protein level in 100 µL cell lysates ( D ) under 10 ng/mL IL-1β stimulation were measured by ELISA. ( E, F ) αB-crystallin protein expression changes under 10 ng/mL IL-1β stimulation were confirmed by immunoblotting ( E ). Densitometric values are presented as ratio normalized to control ( F ). There were 3 to 4 per group in RT-qPCR and ELISA, n = 3 in densitometry, * P < 0.05, ** P < 0.01.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Phosphorylation of αB-Crystallin Involves Interleukin-1β-Mediated Intracellular Retention in Retinal Müller Cells: A New Mechanism Underlying Fibrovascular Membrane Formation

    doi: 10.1167/iovs.64.10.20

    Figure Lengend Snippet: Intracellular αB-crystallin protein level was maintained regardless of IL-1β-induced synthesis suppression in retinal Müller cells. ( A, B ) Dose-dependency ( A ) and time-dependency ( B ) of CRYAB mRNA levels under IL-1β treatment were analyzed by reverse transcription qPCR analyses. ( C, D ) Extracellular αB-crystallin protein level in 2 mL culture medium ( C ) and intracellular αB-crystallin protein level in 100 µL cell lysates ( D ) under 10 ng/mL IL-1β stimulation were measured by ELISA. ( E, F ) αB-crystallin protein expression changes under 10 ng/mL IL-1β stimulation were confirmed by immunoblotting ( E ). Densitometric values are presented as ratio normalized to control ( F ). There were 3 to 4 per group in RT-qPCR and ELISA, n = 3 in densitometry, * P < 0.05, ** P < 0.01.

    Article Snippet: The protein levels of αB-crystallin in cell lysate and culture medium were determined with the StressXpress Alpha B Crystallin ELISA kit (StressMarq Biosciences, Victoria, Canada) per the manufacturer's instructions.

    Techniques: Reverse Transcription, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Quantitative RT-PCR