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tmrm  (StressMarq)


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    StressMarq tmrm
    Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), <t>TMRM</t> <t>(tetramethylrodamine</t> methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.
    Tmrm, supplied by StressMarq, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmrm/product/StressMarq
    Average 94 stars, based on 2 article reviews
    tmrm - by Bioz Stars, 2025-11
    94/100 stars

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    1) Product Images from "Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors"

    Article Title: Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

    Journal: Cancer Research Communications

    doi: 10.1158/2767-9764.CRC-24-0094

    Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.
    Figure Legend Snippet: Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.

    Techniques Used: Membrane, Expressing, Confocal Microscopy, Imaging, Mass Spectrometry, Isolation, Staining, Fluorescence, Microscopy, Comparison, Multiplex Assay, Immunofluorescence



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    Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), <t>TMRM</t> <t>(tetramethylrodamine</t> methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.
    Tmrm, supplied by StressMarq, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tmrm/product/StressMarq
    Average 94 stars, based on 1 article reviews
    tmrm - by Bioz Stars, 2025-11
    94/100 stars
    		  Buy from Supplier
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    Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), <t>TMRM</t> <t>(tetramethylrodamine</t> methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.
    Pbs Solution, supplied by StressMarq, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/StressMarq
    Average 94 stars, based on 1 article reviews
    pbs solution - by Bioz Stars, 2025-11
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    Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.

    Journal: Cancer Research Communications

    Article Title: Membrane-bound Heat Shock Protein mHsp70 Is Required for Migration and Invasion of Brain Tumors

    doi: 10.1158/2767-9764.CRC-24-0094

    Figure Lengend Snippet: Evaluation of the membrane-bound Hsp70 expression in malignant brain tumors by live-cell confocal microscopy imaging. A, Schematic description of the study of brain tumor samples to determine the expression of membrane-associated chaperone in cancer cells, followed by mass spectrometry analysis of isolated lipid rafts. B, Representative confocal microscopy images of the brain tumor samples stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red), disialoganglioside GD2 (red), and protoporphyrin IX (pPIX; magenta). Nuclei were stained by DAPI. Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients are presented. C, Representative confocal microscopy images of the brain metastasis stained for Hsp70 (green), TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images (postcontrast T1-weighted and FLAIR images) of the patients as well as hematoxylin and eosin staining of tumor samples are presented. D, Top row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of patients with GBM ( n = 11) with subsequent Benjamini–Yekutieli test for multiple pairwise comparison of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. Bottom row—Fluorescence intensity measurements of mHsp70 expression in live-cell microscopy images of brain metastasis ( n = 3) with subsequent Benjamini–Yekutieli test for multiple pairwise comparisons of protein expression levels. The bar graph shows the median ± 95% CI (Man–Whitney test result—****, P < 0.0001). The heat map shows P values as the result of a post hoc multiple comparison test. E, Representative multiplex analysis of the immunofluorescence of brain tumor sample stained for Hsp70, SOX2, and nestin. Nuclei were stained by DAPI. NA, necrotic area; VT, vital tissues. Scale bars, 200 μm. F, Representative confocal microscopy images of the normal brain tissue samples stained for Hsp70 (green) and TMRM (tetramethylrodamine methyl ester; red). Nuclei were stained by DAPI (blue). Scale bars, 100 μm. Respective MR images of the patients are presented. G, Live-confocal microscopy images of mHsp70-positive tumor cells in oncostreams detected in GBM samples. Tumor sections were stained for Hsp70 (green), TMRM (red), and DAPI (blue) for nuclei. Scale bars, 100 μm. For the descriptive characteristics of patients, see Supplementary Table S1.

    Article Snippet: The resulting material was washed three times in a PBS solution at room temperature, gently shaking for 10 minutes, and stained for 1 hour with a mixture of TMRM (tetramethylrodamine methyl ester) dyes (1 μmol/L), Hoechst 33342 (0.1 μg/mL), and monoclonal antibodies against Hsp70 [SPA 810 (StressMarq)] conjugated with FITC, to identify the membrane potential of mitochondria, DNA, and membrane-bound Hsp70, respectively.

    Techniques: Membrane, Expressing, Confocal Microscopy, Imaging, Mass Spectrometry, Isolation, Staining, Fluorescence, Microscopy, Comparison, Multiplex Assay, Immunofluorescence