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bafilomycin  (StressMarq)


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    StressMarq bafilomycin
    Bafilomycin, supplied by StressMarq, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bafilomycin/product/StressMarq
    Average 91 stars, based on 4 article reviews
    bafilomycin - by Bioz Stars, 2025-11
    91/100 stars

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    Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with <t>BafA1,</t> a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.
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    Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with BafA1, a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.

    Journal: Autophagy

    Article Title: Role of OSGIN1 in mediating smoking-induced autophagy in the human airway epithelium

    doi: 10.1080/15548627.2017.1301327

    Figure Lengend Snippet: Effect of OSGIN1 on the autophagic flux. OSGIN1 was either overexpressed by lentiviral vector or suppressed by siRNA in the primary basal cells and then treated with BafA1, a late stage inhibitor of autophagic flux. The changes of autophagy markers MAP1LC3B and SQSTM1 were assessed by western analysis with ACTB as a loading control. (A) Overexpression of OSGIN1 with BafA1 treatment. Fold-changes between lenti-OSGIN1 and lenti-control from 3 independent experiments are shown. Note the consistent upregulation of SQSTM1 and MAP1LC3B-II by lenti-OSGIN1 with or without BafA1 (all fold-changes >1). (B) Knockdown OSGIN1 with BafA1 treatment. Folds changes between si-OSGIN1 and si-control from 3 independent experiments are shown. Note there are no consistent changes.

    Article Snippet: 12,15,17,21 BafA1 (StressMarq, SKC-135) was used (100 nM, 2 h) to block the late-stage autophagic flux.

    Techniques: Plasmid Preparation, Western Blot, Over Expression