mouse embryonic fibroblasts  (ATCC)


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    ATCC mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cf 1 scrc 1040  (ATCC)


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    ATCC mef cf 1 scrc 1040
    Mef Cf 1 Scrc 1040, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts  (ATCC)


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    ATCC mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    geltrex  (ATCC)


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    ATCC geltrex
    Geltrex, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef feeder layer  (ATCC)


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    ATCC mef feeder layer
    Mef Feeder Layer, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scrc 1040 tm  (ATCC)


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    ATCC scrc 1040 tm
    Scrc 1040 Tm, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mefs  (ATCC)


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    ATCC mefs
    Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse embryonic fibroblasts  (ATCC)


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    ATCC mouse embryonic fibroblasts
    Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mef cells  (ATCC)


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    ATCC mef cells
    Mef Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cf 1 mouse embryonic fibroblasts  (ATCC)


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    ATCC cf 1 mouse embryonic fibroblasts
    J1 mESCs co-cultured with <t>MEFs</t> were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
    Cf 1 Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells"

    Article Title: Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0039329

    J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
    Figure Legend Snippet: J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Techniques Used: Cell Culture, Staining, Activity Assay

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    ATCC mouse embryonic fibroblasts
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    ATCC cf 1 mouse embryonic fibroblasts
    J1 mESCs co-cultured with <t>MEFs</t> were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.
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    Image Search Results


    J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Journal: PLoS ONE

    Article Title: Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells

    doi: 10.1371/journal.pone.0039329

    Figure Lengend Snippet: J1 mESCs co-cultured with MEFs were maintained continuously with vehicle (0.1% DMSO), 1,000 U/ml LIF, 10 or 20 µM SB-216763, 2 µM BIO, or 3 µM CHIR-99021. The cultures were passaged in parallel and 20,000–50,000 mESCs were plated on new MEF-covered 6-well plates every 3 days. A duplicate plate was stained for alkaline phosphatase (AP) activity at days 10, 20, and 30. Shown are representative images from one of two independent trials. Day 60 images were obtained from an independent culture maintained with vehicle (0.1% DMSO), 1,000 U/mL LIF, or 10 µM SB-216763. AP staining on day 60 was carried out on samples from a single trial. The scale bar shown in the day 10 vehicle image represents 100 µm and all images are presented at the same scale.

    Article Snippet: CF-1 mouse embryonic fibroblasts (MEF; SCRC-1040, ATCC) were used as a feeder layer for mESC cell culture.

    Techniques: Cell Culture, Staining, Activity Assay