nf κb sc 8008  (Santa Cruz Biotechnology)

 
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    Santa Cruz Biotechnology nf κb sc 8008
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    nfκb santa cruz biotech cat sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology nfκb santa cruz biotech cat sc 8008
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    tcok sichem sc 8008  (Sirius Fine Chemicals)


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    Sirius Fine Chemicals tcok sichem sc 8008
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    p65 sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology p65 sc 8008
    List of antibodies
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    1) Product Images from "Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice"

    Article Title: Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice

    Journal: Biomolecules & Therapeutics

    doi: 10.4062/biomolther.2024.022

    List of antibodies
    Figure Legend Snippet: List of antibodies

    Techniques Used: Molecular Weight

    Inhibitory ability of ARO on LPS-induced NF-κB activation in BEAS-2B cells. (A) The expression levels of p-NF-κB, p-IκBα, MyD88 and TLR4 in whole cell lysates (WCL) detected using western blotting. BEAS-2B cells were maintained ARO (25–200 μM) for 1 h and incubated in the presence of LPS (1 μg/mL) for 1 h. Quantitative analysis of (B) p-p65, (C) p-IқB, (D) MyD88, and (E) TLR4 was performed using the ImageJ software (version 1.50e; National Institute of Health, MD, USA). Data are expressed as the mean ± SD ( # p <0.01 for comparison with the control; * p <0.05 for comparison with the LPS group). ARO stands for aromadendrin.
    Figure Legend Snippet: Inhibitory ability of ARO on LPS-induced NF-κB activation in BEAS-2B cells. (A) The expression levels of p-NF-κB, p-IκBα, MyD88 and TLR4 in whole cell lysates (WCL) detected using western blotting. BEAS-2B cells were maintained ARO (25–200 μM) for 1 h and incubated in the presence of LPS (1 μg/mL) for 1 h. Quantitative analysis of (B) p-p65, (C) p-IқB, (D) MyD88, and (E) TLR4 was performed using the ImageJ software (version 1.50e; National Institute of Health, MD, USA). Data are expressed as the mean ± SD ( # p <0.01 for comparison with the control; * p <0.05 for comparison with the LPS group). ARO stands for aromadendrin.

    Techniques Used: Activation Assay, Expressing, Western Blot, Incubation, Software, Comparison, Control

    sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology sc 8008
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    sc 8008 pyrimidyl tetrazine af555 jena bioscience  (Jena Bioscience)


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    Jena Bioscience sc 8008 pyrimidyl tetrazine af555 jena bioscience
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    sc 8008 pyrimidyl tetrazine af555 jena bioscience  (Sirius Fine Chemicals)


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    Sirius Fine Chemicals sc 8008 pyrimidyl tetrazine af555 jena bioscience
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    anti nf κb p65 catalog no sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology anti nf κb p65 catalog no sc 8008
    Anti Nf κb P65 Catalog No Sc 8008, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti nf κb p65 catalog no sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology anti nf κb p65 catalog no sc 8008
    Primer sequences of specific genes
    Anti Nf κb P65 Catalog No Sc 8008, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Renoprotective effect of a novel combination of 6-gingerol and metformin in high-fat diet/streptozotocin-induced diabetic nephropathy in rats via targeting miRNA-146a, miRNA-223, TLR4/TRAF6/NLRP3 inflammasome pathway and HIF-1α"

    Article Title: Renoprotective effect of a novel combination of 6-gingerol and metformin in high-fat diet/streptozotocin-induced diabetic nephropathy in rats via targeting miRNA-146a, miRNA-223, TLR4/TRAF6/NLRP3 inflammasome pathway and HIF-1α

    Journal: Biological Research

    doi: 10.1186/s40659-024-00527-9

    Primer sequences of specific genes
    Figure Legend Snippet: Primer sequences of specific genes

    Techniques Used: Sequencing

    GR, MET, and their combination down-regulated TLR4/TRAF6/NLRP3 inflammasome pathway gene expression in DN Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure mRNA relative expression of TLR4 ( A ), TRAF6 ( B ), NF-κB (p65) ( C ), NLRP3 ( D ), and caspase-1 ( E ) in renal tissue. DN: diabetic nephropathy; GR: 6-gingerol; MET: metformin; TLR4: Toll-like receptor 4; TRAF6: Tumor necrosis factor receptor-associated Factor 6; NF-κB (p65): Nuclear factor-kappa B (p65); NLRP3: NOD-like receptor family pyrin domain-containing 3. Data are represented as Mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \pm $$\end{document} SEM (***p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ < $$\end{document} 0.001, ****p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ <$$\end{document} 0.0001, ns: non-significant)
    Figure Legend Snippet: GR, MET, and their combination down-regulated TLR4/TRAF6/NLRP3 inflammasome pathway gene expression in DN Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure mRNA relative expression of TLR4 ( A ), TRAF6 ( B ), NF-κB (p65) ( C ), NLRP3 ( D ), and caspase-1 ( E ) in renal tissue. DN: diabetic nephropathy; GR: 6-gingerol; MET: metformin; TLR4: Toll-like receptor 4; TRAF6: Tumor necrosis factor receptor-associated Factor 6; NF-κB (p65): Nuclear factor-kappa B (p65); NLRP3: NOD-like receptor family pyrin domain-containing 3. Data are represented as Mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \pm $$\end{document} SEM (***p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ < $$\end{document} 0.001, ****p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ <$$\end{document} 0.0001, ns: non-significant)

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Immunohistochemical examination of NF-κB (p65) in renal tissue A : Microscopic images of immunostained renal sections against NF-κB (p65) showing negative staining in the cortex and medulla in the control group, excess brown tubular staining in the cortex and medulla in the DN group, decreased brown tubular staining in the cortex and medulla in GR group and MET group, and much more decreased brown tubular staining in the cortex and medulla in GR + MET group. Black arrows refer to nuclear staining. Immunohistochemistry is counterstained with Mayer’s hematoxylin. X: 400, scale bar = 50 micrometer B : Immunostaining of NF-κB (p65) was assessed quantitatively through the area of positive expression. DN: diabetic nephropathy, GR: 6-gingerol, MET: metformin, NF-κB (p65): nuclear factor-kappa B (p65). Data are represented as Mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \pm $$\end{document} SEM (****p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ <$$\end{document} 0.0001, ns: non-significant)
    Figure Legend Snippet: Immunohistochemical examination of NF-κB (p65) in renal tissue A : Microscopic images of immunostained renal sections against NF-κB (p65) showing negative staining in the cortex and medulla in the control group, excess brown tubular staining in the cortex and medulla in the DN group, decreased brown tubular staining in the cortex and medulla in GR group and MET group, and much more decreased brown tubular staining in the cortex and medulla in GR + MET group. Black arrows refer to nuclear staining. Immunohistochemistry is counterstained with Mayer’s hematoxylin. X: 400, scale bar = 50 micrometer B : Immunostaining of NF-κB (p65) was assessed quantitatively through the area of positive expression. DN: diabetic nephropathy, GR: 6-gingerol, MET: metformin, NF-κB (p65): nuclear factor-kappa B (p65). Data are represented as Mean \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ \pm $$\end{document} SEM (****p \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$ <$$\end{document} 0.0001, ns: non-significant)

    Techniques Used: Immunohistochemical staining, Negative Staining, Control, Staining, Immunohistochemistry, Immunostaining, Expressing

    A summary figure of the mechanisms of action of GR, MET, and their combination in HFD/STZ-induced DN in rats DN: diabetic nephropathy; GR: 6-gingerol; MET: metformin; HFD: high-fat diet; STZ: streptozotocin; TLR4: Toll-like receptor 4; TRAF6: tumor necrosis factor receptor-associated Factor 6; NF-κB (p65): nuclear factor-kappa B (p65); NLRP3: NOD-like receptor family pyrin domain-containing 3; TNF-α: tumor necrosis factor-alpha; IL-1β: interleukin-1 beta; ROS: reactive oxygen species; GSH: reduced glutathione; MDA: malondialdehyde; HIF-1α: hypoxia-inducible factor-1 alpha.
    Figure Legend Snippet: A summary figure of the mechanisms of action of GR, MET, and their combination in HFD/STZ-induced DN in rats DN: diabetic nephropathy; GR: 6-gingerol; MET: metformin; HFD: high-fat diet; STZ: streptozotocin; TLR4: Toll-like receptor 4; TRAF6: tumor necrosis factor receptor-associated Factor 6; NF-κB (p65): nuclear factor-kappa B (p65); NLRP3: NOD-like receptor family pyrin domain-containing 3; TNF-α: tumor necrosis factor-alpha; IL-1β: interleukin-1 beta; ROS: reactive oxygen species; GSH: reduced glutathione; MDA: malondialdehyde; HIF-1α: hypoxia-inducible factor-1 alpha.

    Techniques Used:

    p65 sc 8008  (Santa Cruz Biotechnology)

     
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    Santa Cruz Biotechnology p65 sc 8008
    P65 Sc 8008, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    List of antibodies

    Journal: Biomolecules & Therapeutics

    Article Title: Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice

    doi: 10.4062/biomolther.2024.022

    Figure Lengend Snippet: List of antibodies

    Article Snippet: p65 (sc-8008) , Santa Cruz , 65 , 1:1000 , Mouse , Goat anti mouse-HRP.

    Techniques: Molecular Weight

    Inhibitory ability of ARO on LPS-induced NF-κB activation in BEAS-2B cells. (A) The expression levels of p-NF-κB, p-IκBα, MyD88 and TLR4 in whole cell lysates (WCL) detected using western blotting. BEAS-2B cells were maintained ARO (25–200 μM) for 1 h and incubated in the presence of LPS (1 μg/mL) for 1 h. Quantitative analysis of (B) p-p65, (C) p-IқB, (D) MyD88, and (E) TLR4 was performed using the ImageJ software (version 1.50e; National Institute of Health, MD, USA). Data are expressed as the mean ± SD ( # p <0.01 for comparison with the control; * p <0.05 for comparison with the LPS group). ARO stands for aromadendrin.

    Journal: Biomolecules & Therapeutics

    Article Title: Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice

    doi: 10.4062/biomolther.2024.022

    Figure Lengend Snippet: Inhibitory ability of ARO on LPS-induced NF-κB activation in BEAS-2B cells. (A) The expression levels of p-NF-κB, p-IκBα, MyD88 and TLR4 in whole cell lysates (WCL) detected using western blotting. BEAS-2B cells were maintained ARO (25–200 μM) for 1 h and incubated in the presence of LPS (1 μg/mL) for 1 h. Quantitative analysis of (B) p-p65, (C) p-IқB, (D) MyD88, and (E) TLR4 was performed using the ImageJ software (version 1.50e; National Institute of Health, MD, USA). Data are expressed as the mean ± SD ( # p <0.01 for comparison with the control; * p <0.05 for comparison with the LPS group). ARO stands for aromadendrin.

    Article Snippet: p65 (sc-8008) , Santa Cruz , 65 , 1:1000 , Mouse , Goat anti mouse-HRP.

    Techniques: Activation Assay, Expressing, Western Blot, Incubation, Software, Comparison, Control