alexa fluor 546  (New England Biolabs)


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    Structured Review

    New England Biolabs alexa fluor 546
    HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 <t>(Alexa</t> 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.
    Alexa Fluor 546, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 546/product/New England Biolabs
    Average 94 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 546 - by Bioz Stars, 2022-11
    94/100 stars

    Images

    1) Product Images from "Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling"

    Article Title: Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling

    Journal: bioRxiv

    doi: 10.1101/2022.05.13.491788

    HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.
    Figure Legend Snippet: HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.

    Techniques Used: Construct, Labeling, SDS Page, Staining

    2) Product Images from "Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling"

    Article Title: Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling

    Journal: bioRxiv

    doi: 10.1101/2022.05.13.491788

    HPO-30 ICD binds to the side of actin filaments and slows down actin polymerization. A) Top, still frames of several capping events between SNAP-CapZ-488 (5nM, green) and actin (red). Bottom, still frames of a side-binding event between SNAP-FKBP-HPO-30-546/SNAP-FRB-HPO-30 (15nM, cyan) and actin (red). The images in top and bottom are not to the same scale. B) Left, Quantification of the frequency of side binding events from smTIRF videos. Right, quantification of the frequency of filaments with 0 events, 1 event, and 2 events. n=3 videos, at least 15 filaments per video. * = p
    Figure Legend Snippet: HPO-30 ICD binds to the side of actin filaments and slows down actin polymerization. A) Top, still frames of several capping events between SNAP-CapZ-488 (5nM, green) and actin (red). Bottom, still frames of a side-binding event between SNAP-FKBP-HPO-30-546/SNAP-FRB-HPO-30 (15nM, cyan) and actin (red). The images in top and bottom are not to the same scale. B) Left, Quantification of the frequency of side binding events from smTIRF videos. Right, quantification of the frequency of filaments with 0 events, 1 event, and 2 events. n=3 videos, at least 15 filaments per video. * = p

    Techniques Used: Binding Assay

    HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.
    Figure Legend Snippet: HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.

    Techniques Used: Construct, Labeling, SDS Page, Staining

    3) Product Images from "An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates"

    Article Title: An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates

    Journal: Analytical biochemistry

    doi: 10.1016/j.ab.2016.05.026

    Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored
    Figure Legend Snippet: Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored

    Techniques Used: Activity Assay, Fluorescence, Labeling, Purification

    4) Product Images from "An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates"

    Article Title: An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates

    Journal: Analytical biochemistry

    doi: 10.1016/j.ab.2016.05.026

    Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored
    Figure Legend Snippet: Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored

    Techniques Used: Activity Assay, Fluorescence, Labeling, Purification

    5) Product Images from "An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates"

    Article Title: An assay for 26S proteasome activity based on fluorescence anisotropy measurements of dye-labeled protein substrates

    Journal: Analytical biochemistry

    doi: 10.1016/j.ab.2016.05.026

    Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored
    Figure Legend Snippet: Monitoring 26S proteasome activity by fluorescence anisotropy. Degradation of the polyubiquitinated Alexa Fluor 546-labeled substrate by purified yeast proteasome as monitored by fluorescence anisotropy measurements. The degradation reaction was monitored

    Techniques Used: Activity Assay, Fluorescence, Labeling, Purification

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  • 94
    New England Biolabs alexa fluor 546
    HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 <t>(Alexa</t> 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.
    Alexa Fluor 546, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 546/product/New England Biolabs
    Average 94 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 546 - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

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    HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.

    Journal: bioRxiv

    Article Title: Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling

    doi: 10.1101/2022.05.13.491788

    Figure Lengend Snippet: HPO-30 ICD inhibits actin polymerization and depolymerization. A) Immobilized WRC-2MBP pulling down GB1-tagged and SNAP-tagged HPO-30 constructs. Abbreviations are as used before. Bait: 60 pmol, Prey: 600 pmol, 50 mM NaCl, 10 mM HEPES pH 7.0, 5% glycerol. B) SNAP-tag labeling of CapZ, FKBP-HPO-30, and FKBP with SNAP-Surface© AlexaFluor©-488 (Alexa 488), SNAP-Surface© AlexaFluor©-546 (Alexa 546), and SNAP-Surface© AlexaFluor©-647 (Alexa 647). Left, SDS-PAGE gel visualized with Alexa-488 filter. Right, Coomassie blue-stained SDS-PAGE gel. C) Actin depolymerization assays using SNAP-Alexa labeled proteins. 5 µM actin diluted 20-fold with 25% 50KMEH5Gd/75%GMg. 5 µM rapamycin or equivalent volume of DMSO added. Labeled proteins are denoted by the number associated with the dye they are labeled with. Colored bars represent wild type protein.

    Article Snippet: Protein labeling efficiency was calculated by dividing protein concentration by dye concentration– for Alexa Fluor® 488 the labeling efficiency was estimated at ∼100%, for Alexa Fluor® 546 the labeling efficiency was estimated at ∼60%.

    Techniques: Construct, Labeling, SDS Page, Staining

    HPO-30 ICD binds to the side of actin filaments and slows down actin polymerization. A) Top, still frames of several capping events between SNAP-CapZ-488 (5nM, green) and actin (red). Bottom, still frames of a side-binding event between SNAP-FKBP-HPO-30-546/SNAP-FRB-HPO-30 (15nM, cyan) and actin (red). The images in top and bottom are not to the same scale. B) Left, Quantification of the frequency of side binding events from smTIRF videos. Right, quantification of the frequency of filaments with 0 events, 1 event, and 2 events. n=3 videos, at least 15 filaments per video. * = p

    Journal: bioRxiv

    Article Title: Dendrite branching receptor HPO-30 uses two novel mechanisms to regulate actin cytoskeletal remodeling

    doi: 10.1101/2022.05.13.491788

    Figure Lengend Snippet: HPO-30 ICD binds to the side of actin filaments and slows down actin polymerization. A) Top, still frames of several capping events between SNAP-CapZ-488 (5nM, green) and actin (red). Bottom, still frames of a side-binding event between SNAP-FKBP-HPO-30-546/SNAP-FRB-HPO-30 (15nM, cyan) and actin (red). The images in top and bottom are not to the same scale. B) Left, Quantification of the frequency of side binding events from smTIRF videos. Right, quantification of the frequency of filaments with 0 events, 1 event, and 2 events. n=3 videos, at least 15 filaments per video. * = p

    Article Snippet: Protein Labeling SNAP-tagged proteins were labeled with SNAP-Surface® Alexa Fluor® 488, SNAP-Surface® Alexa Fluor® 546, and SNAP-Surface® Alexa Fluor® 647 from New England Biolabs.

    Techniques: Binding Assay