goat anti rabbit igg magnetic beads  (New England Biolabs)


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    Name:
    Goat Anti Rabbit IgG Magnetic Beads
    Description:
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    Catalog Number:
    s1432s
    Price:
    184
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    New England Biolabs goat anti rabbit igg magnetic beads
    Goat Anti Rabbit IgG Magnetic Beads
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/goat anti rabbit igg magnetic beads/product/New England Biolabs
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg magnetic beads - by Bioz Stars, 2020-01
    85/100 stars

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    Related Articles

    In Vitro:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: For MeRIP, 300 μg of total RNA or 10 μg in vitro transcribed EV71 RNA were incubated with an anti-m6 A antibody (Synaptic Systems, Goettingen, Germany) or a IgG antibody in 300 μl of immunoprecipitation (IP) Buffer (150 mM NaCl, 0.1% NP-40, 10 mM Tris–HCl, pH 7.4) for 2 h at 4°C. .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Magnetic Beads:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C. .. Beads were washed six times with 500 μl of IP buffer and then incubated with 300 μl of elution buffer (5 mM Tris–HCl, pH 7.5, 1 mM EDTA, pH 8.0, 0.05% sodium dodecyl sulfate (SDS), and 4.2 μl of 20 mg/ml proteinase K) for 1.5 h at 50°C.

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: .. PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)). .. The preformed antibody-antigen complex was added to the beads and incubated for 1 h (in the case of HEK-293 cell experiments) or 3 h (in the case of mouse brain experiments) at 4 °C with gentle rotation.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

    Protease Inhibitor:

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: Briefly, 20 Drosophila L3 larvae were ground in 600 µl PBS supplemented with 1 m m PMSF, 1 m m EDTA, 1 m m DTT and 0.1 m m protease inhibitor cocktail (Sigma P8340) and then further homogenized by the addition of 1-mm glass beads and vortexing at a high speed for 5 min. .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed.

    Northern Blot:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: Paragraph title: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting ... The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Infection:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting Total RNA was extracted from Vero cells infected with strain EV71-XF at a multiplicity of infection (MOI) of 0.1 using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Acid Assay:

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Protein concentration was determined by a bicinchoninic acid assay (BioRad). .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Immunoprecipitation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: Paragraph title: m6 A-Methylated RNA immunoprecipitation (MeRIP) and Northern blotting ... The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: Paragraph title: Immunoprecipitation ... PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)).

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Paragraph title: Immunoprecipitation ... Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Incubation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C. .. Beads were washed six times with 500 μl of IP buffer and then incubated with 300 μl of elution buffer (5 mM Tris–HCl, pH 7.5, 1 mM EDTA, pH 8.0, 0.05% sodium dodecyl sulfate (SDS), and 4.2 μl of 20 mg/ml proteinase K) for 1.5 h at 50°C.

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Article Title: Therapeutic Molecules and Endogenous Ligands Regulate the Interaction between Brain Cellular Prion Protein (PrPC) and Metabotropic Glutamate Receptor 5 (mGluR5)
    Article Snippet: One microgram of capture antibody was incubated overnight at 4 °C with 1 mg of detergent-solubilized lysate protein with continuous mixing. .. PureProteome protein A/G mix magnetic beads (Millipore, catalog no. LSKMAGAG10) or goat anti-rabbit IgG magnetic beads (New England Biolabs, catalog no. S1432S) were washed in wash buffer (PBS and 0.1% Tween 20 (pH 7.4)).

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

    Expressing:

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: Extracts of Drosophila expressing different N-terminal HTT peptides were tested for their ability to seed the formation of thioflavinT-positive amyloid using an ASA ( ) with modifications. .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed.

    Protein Concentration:

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Protein concentration was determined by a bicinchoninic acid assay (BioRad). .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min.

    Western Blot:

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The protein complex was released from the beads in 2× protein loading buffer (BioRad) at 99 °C for 10 min followed by western blot analysis.

    Binding Assay:

    Article Title: Comparative study of naturally occurring huntingtin fragments in Drosophila points to exon 1 as the most pathogenic species in Huntington's disease
    Article Snippet: .. 100 μg of magnetic beads (S1432S NEB, binding capacity 10 μg) conjugated to anti-rabbit IgG secondary antibody were washed with PBS buffer and incubated with a solution of 10 μg of A11 antibody (AB9234, Millipore) in 1 ml of 0.1% BSA in PBS buffer at RT with continuous shaking at low speed. .. After a 2-h incubation, magnetic beads were removed and washed three times with PBST buffer (1 ml) and resuspended in 1 ml of PBS.

    Plasmid Preparation:

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: In vitro EV71 RNA was transcribed from a cDNA plasmid ( ) linearized by HindIII using the MEGAscript® T7 Kit (Ambion, Austin, TX, USA) according to the manufacturer's protocols. .. The mixture was then incubated with 20 μl of anti-rabbit antibody conjugated magnetic beads (NEB, Ipswich, MA, USA; S1432S), which were then washed three times with 500 μl of IP buffer, followed by rotating for 2 h at 4°C.

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  • 85
    New England Biolabs goat anti rabbit igg magnetic beads
    Goat Anti Rabbit Igg Magnetic Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg magnetic beads/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg magnetic beads - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

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