anti rabbit igg antibody  (New England Biolabs)


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    Name:
    Goat Anti Rabbit IgG Magnetic Beads
    Description:
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    Catalog Number:
    s1432s
    Price:
    188
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs anti rabbit igg antibody
    Goat Anti Rabbit IgG Magnetic Beads
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/anti rabbit igg antibody/product/New England Biolabs
    Average 95 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg antibody - by Bioz Stars, 2021-01
    95/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Fetal lung C4BPA induces p100 processing in human placenta
    Article Snippet: .. Immunoprecipitation of exosomes We coupled goat-anti-rabbit immunomagnetic beads (New England Biolabs, USA) coated with anti-AFP or C4BPA antibody by mixing them and rotating at 4 °C for 1 hr. .. The beads were washed three times with immunoprecipitation buffer (IP buffer; 10 mM Tris [pH 7.2], 10 mM MgCl2 , 200 mM KCl, 0.1% Triton X-100).

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Incubation:

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

    RNA Sequencing Assay:

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Blocking Assay:

    Article Title: In vivo effects of rosiglitazone in a human neuroblastoma xenograft
    Article Snippet: .. After blocking (5% skimmed milk for 2 h), nitrocellulose membranes were washed and then immunostained with a rabbit anti-human PPARγ antibody (1 : 1000) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or with an anti-phospho-PPARγ (Ser82) clone AW 504 (1 : 1000) (Upstate, Lake Placid, NY, USA), followed by a secondary anti-rabbit IgG antibody (1 : 2000) (New England Biolabs, Beverly, MA, USA). .. The antibody-reacted proteins were revealed by LumiGLO chemiluminescent reagent and peroxide (New England Biolabs).

    Magnetic Beads:

    Article Title: Disturbed flow increases UBE2C via loss of miR-483-3p, inducing aortic valve calcification by the HIF1α pathway in endothelial cells
    Article Snippet: .. For pVHL or ubiquitin immunoprecitations, 25 μl of goat-Anti-Rabbit IgG (New England Biolabs, S1432S) or goat-Anti-Mouse IgG (New England Biolabs, S1431S) magnetic beads were pre-incubated (2 hour at RT) with 5 μg of anti-VHL antibody (Thermo Fisher Scientific PA5–27322) or an anti-total-ubiquitin antibody (Abcam ab128424). .. Cells were lysed using an IP lysis buffer (Thermo Fisher Scientific 87787) and added to the magnetic beads overnight at 4C.

    Article Title: Methyltransferase-like 3 modulates severe acute respiratory syndrome coronavirus-2 RNA N6-methyladenosine modification and replication
    Article Snippet: .. Thirty-five microliters magnetic beads (NEB; goat anti-rabbit magnetic beads; cat. no. S1432S) were added, and samples were washed three times and rotated 2 h at 4°C. .. The beads were washed six times and incubated with 300 μL elution buffer (5 mM Tris-HCl [pH 7.5], 1 mM EDTA [pH 8.0], 0.05% SDS, and 8.4 μL of 10 mg/mL proteinase K) for 1.5 h at 50°C.

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Article Title: ZNT7 binds to CD40 and influences CD154‐triggered p38 MAPK activity in B lymphocytes—a possible regulatory mechanism for zinc in immune function
    Article Snippet: .. Goat anti‐(rabbit IgG) magnetic beads (200 μg, New England BioLabs, Ipswich, MA, USA) were added and incubated at RT for another 30 min. .. The precleared lysate was then incubated with a ZNT7 antibody (1 μg) at RT for 1 h. Prewashed magnetic beads (200 μg) were then added and incubated overnight at 4 °C.

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  • 95
    New England Biolabs anti rabbit igg antibody
    Anti Rabbit Igg Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg antibody/product/New England Biolabs
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg antibody - by Bioz Stars, 2021-01
    95/100 stars
      Buy from Supplier

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