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Streptavidin Magnetic Beads

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Streptavidin Magnetic Beads 5 ml

Catalog Number:

S1420S

Price:

303

Size:

5 ml

Category:

Magnetic Separation Equipment

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New England Biolabs streptavidin magnetic beads

Streptavidin Magnetic Beads 5 ml
https://www.bioz.com/result/streptavidin magnetic beads/product/New England Biolabs
Average 99 stars, based on 93 article reviews
Price from $9.99 to $1999.99

streptavidin magnetic beads - by Bioz Stars, 2019-08

99/100 stars

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Clone Assay:

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: MARCH5 wild-type and MARCH5H43W coding sequences were cloned into MCS-BirA(R118G)-HA vector ( , ; a gift from Kyle Roux; Addgene plasmid 36047) by using Nhe I and Bam HI restriction sites. .. Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight.

Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], streptavidin-coated magnetic beads provide the strongest and irreversible binding capability to immobilize the drivers, which ensures the elimination of hybridized common mRNAs. (c) The protocol does not require PCR cloning for subtractive library construction. .. This is beneficial because it prevents developing subtractive libraries with larger capacities of clones as previously reported [ , , , - ]. (d) There is no need for radioisotopes and/or some related specialized enzymes such as [α-32 P]dCTP [ , ] and terminal transferase [ ].

Centrifugation:

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: Cell lysates were cleared by centrifugation at 40,000 rpm in a Beckman L-80 ultracentrifuge at room temperature for 30 min using Ti 70.1 rotor. .. The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences).

Amplification:

Article Title: Microdroplet-based PCR amplification for large scale targeted sequencing
Article Snippet: 110 μL of streptavidin beads (NEB, S1420S) in a 1.5 mL microcentrifuge tube are spun briefly in a microcentrifuge and placed on a magnetic separation rack (NEB, S1506S) for 30 seconds, the supernatant is removed and discarded. .. The beads are resuspended in 110 μL of binding buffer (0.5 M NaCl, 20 mM Tris-HCl (pH 7.5), 1 mM EDTA) by vortexing, briefly spun in a microcentrifuge and placed on a magnetic separation rack for 30 seconds, the supernatant is removed and discarded.

Synthesized:

Article Title: An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis
Article Snippet: Paragraph title: Metabolic Labeling of Newly Synthesized RNA ... Biotinylated RNA was then was separated from unlabeled RNA using streptavidin-coated magnetic beads (New England Biolabs).

Construct:

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: The constructs MARCH5-BirA(R118G)-HA, MARCH5H43W -BirA(R118G)-HA, and the BirA(R118G)-HA (as a control) were transfected into cells using Lipofectamine 2000 transfection reagent (Life Technologies). .. Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight.

Enzyme-linked Immunosorbent Assay:

Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6
Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C. .. After washing the beads thoroughly with PBS, mouse serum was diluted 1:20 in PBS and incubated with the beads for 4 h at room temperature.

Incubation:

Article Title: Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1
Article Snippet: The streptavidin magnetic beads were resuspended in 200 µl of protease K buffer follow by the addition of Protease K (Ambion) to the final concentration of 1.2 mg/ml. .. After incubation at 55°C for 30 minutes, the supernatant was transferred to a new tube and another 200 µl of protease K buffer was added to the streptavidin magnetic beads. .. The incubation was continued for 5 minutes at 55°C before the supernatant was combined and the RNA was recovered by acidic phenol/chloroform extraction followed by a chloroform extraction and an ethanol precipitation.

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight. .. After incubation, samples were washed five times with IP buffer and then processed for and analyzed by mass spectrometry to identify eluted proteins.

Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses
Article Snippet: The combined DNA lysate mixture was incubated at 37 °C for 1 h and then quenched by addition of an equal volume of quenching/bead binding buffer (40 mM Tris⋅HCl, pH 7.6, 200 mM NaCl, 2 mM DTT, 15% PEG 6000, 20 mM EDTA). .. Streptavidin-coated magnetic beads (NEB) were used to capture and separate the biotinylated SP8 DNA from endogenous nucleic acids.

Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis
Article Snippet: After the annealing reaction streptavidin-coated magnetic beads (20μl of a 10mg/ml stock solution) (New England Biolabs, Beverly, MA, USA) that were pre-washed (1xSSC/1% Triton X-100, coated with 5x Denhardt’s for 30min at RT) and resuspended in 1x SSC were added to the reaction mixture. .. After the annealing reaction streptavidin-coated magnetic beads (20μl of a 10mg/ml stock solution) (New England Biolabs, Beverly, MA, USA) that were pre-washed (1xSSC/1% Triton X-100, coated with 5x Denhardt’s for 30min at RT) and resuspended in 1x SSC were added to the reaction mixture.

Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
Article Snippet: In a typical experiment, 1 μM synthetic rU7rC4-biotin was mixed with 1 μM purified NS5A protein in 50 μl binding buffer containing 50 mM HEPES (pH 7.5), 5 mM MgCl2 , and 10 mM ΒME. .. After incubation on ice for 30 min, 50 μl of streptavidin magnetic beads (New England Biolabs) was added, and the mixture was incubated at room temperature for 30 min. .. The supernatant was removed after magnetic collection of the beads.

Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae)
Article Snippet: Extracted genomic DNA was digested with Bsa AI and Hin cII and ligated to SNX linkers using T4 DNA ligase ( ). .. The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Streptavidin-coated magnetic beads (New England Biolabs) were used for recovering single-stranded DNA. .. Double-stranded DNA was generated from the enriched DNA fragments via polymerase chain reaction (PCR) using SNX primers.

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: Cleared lysates were pooled and incubated with Ni-NTA agarose (Qiagen, 500 μL slurry pre-equilibrated in buffer 1) for 3 h at room temperature. .. The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences).

Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6
Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C.

Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay
Article Snippet: A PA-TEM8-mCit binding assay consisted of mixing PAE733C labeled with biotin-PEG2 -maleimide (Pierce; labeled according to manufacturer protocols) with TEM8-mCit at 1 μM each in HiHBST. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of streptavidin functionalized magnetic beads (New England Biolabs) for an additional 15 minutes. .. Magnetic beads were pulled down with a strong magnet and subsequently washed 4 × 150 μl with HiHBST.

Article Title: Microdroplet-based PCR amplification for large scale targeted sequencing
Article Snippet: 110 μL of streptavidin beads (NEB, S1420S) in a 1.5 mL microcentrifuge tube are spun briefly in a microcentrifuge and placed on a magnetic separation rack (NEB, S1506S) for 30 seconds, the supernatant is removed and discarded. .. The washed beads are resuspended in 110 μL of binding buffer and 50 μL of the beads are added to the broken amplicon emulsion.

Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
Article Snippet: After 10 min incubation at 70°C, the mRNA/primer mixture was transferred to 45°C and mixed with 5× first-strand buffer, 25 mM MgCl2 , 10 mM deoxyribonucleotide triphosphate (dNTP) mix, and ImProm-II™ Reverse Transcriptase (Promega, Madison, WI, USA) at the total volume of 40 μl. .. The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed streptavidin-coated magnetic beads (1.1 mg/ml; New England BioLabs, Ipswich, MA, USA).

Article Title: Removing the needle from the haystack: Enrichment of Wolbachia endosymbiont transcripts from host nematode RNA by Cappable-seq™
Article Snippet: This mix was added to 100 μL of hydrophilic streptavidin magnetic beads (New England Biolabs) previously washed twice with wash Buffer B (10 mM Tris-HCl, pH 7.5, 50 mM NaCl) and twice with wash Buffer A (100 μL/wash). .. This mix was added to 100 μL of hydrophilic streptavidin magnetic beads (New England Biolabs) previously washed twice with wash Buffer B (10 mM Tris-HCl, pH 7.5, 50 mM NaCl) and twice with wash Buffer A (100 μL/wash).

Expressing:

Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses
Article Snippet: Expression of each gene product candidate was confirmed by SDS/PAGE analysis. .. Streptavidin-coated magnetic beads (NEB) were used to capture and separate the biotinylated SP8 DNA from endogenous nucleic acids.

Western Blot:

Article Title: Association of UHRF1 with H3K9 methylation directs the maintenance of DNA methylation
Article Snippet: A 50 µL slurry of streptavidin magnetic beads (NEB) was equilibrated in binding buffer containing 50 mM Tris-HCl, pH 8.0, 300 mM NaCl, and 0.1% NP-40 before being saturated with 1 nmole biotinylated peptide for 1 hour at 4°C with rotation. .. Unbound peptide was washed with binding buffer, and 100 pmoles of protein in binding buffer supplemented with 0.5% bovine serum albumin (BSA) (w/v) was incubated for 3 hours at 4°C with rotation.

Article Title: Molecular Characterization of Zebrafish Oatp1d1 (Slco1d
Article Snippet: Oatp1d1 is present in three forms: as a monomeric protein of ∼80 kDa, as a dimeric form of ∼150 kDa, and as a tetramer or higher order oligomer that appears at ∼250 kDa in Western blots from the total cell lysate fraction ( A ). .. However, when the cell membrane fraction was isolated through the cell surface biotinylation, followed by binding of membrane fraction to the streptavidin magnetic beads, Western blots with anti-His primary antibody showed only the oligomeric form ( A ). .. Oligomers were not formed through disulfide bonds, because there was no breakage of the oligomeric complex in the presence of dithiothreitol (DTT) as a reducing agent ( B ).

Transformation Assay:

Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae)
Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Streptavidin-coated magnetic beads (New England Biolabs) were used for recovering single-stranded DNA. .. The PCR products were then digested with the restriction enzyme Nhe I (New Engl and Biolabs) and ligated into the pUC19 plasmid (digested with Xba I and dephosphorylated).

Hybridization:

Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes
Article Snippet: To isolate SSR-containing fragments, the probes were attached to Streptavidin magnetic beads (New England Biolabs, Ipswitch, MA) according to the manufacturer’s instructions. .. To isolate SSR-containing fragments, the probes were attached to Streptavidin magnetic beads (New England Biolabs, Ipswitch, MA) according to the manufacturer’s instructions.

Transfection:

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight.

Concentration Assay:

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences). .. The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences).

Protease Inhibitor:

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: Proteins were eluted in 8 mL of buffer 2 (8 M urea, 200 mM NaCl, 2% SDS, 50 mM sodium phosphate, 10 mM EDTA, 100 mM Tris-HCl at pH 4.3, and EDTA-free protease inhibitor mix for His-Tag sequences [RPI]). .. The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences). .. After incubation overnight at room temperature, the streptavidin magnetic beads were washed in 3 × 500 μL of buffer 3, 3 × 500 μL of buffer 3 with 2% SDS, 3 × 500 μL of buffer 3 without SDS, and then 3 × 500 μL of T1 ribonuclease buffer (150 mM KCl, 2 mM EDTA, 0.5 mM DTT, 50 mM Tris-HCl at pH 7.4, and EDTA-free protease inhibitor mix for His-Tag sequences [RPI]).

Cell Culture:

Proximity Assay:

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: Paragraph title: BioID proximity assay ... Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight.

Polymerase Chain Reaction:

Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae)
Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Streptavidin-coated magnetic beads (New England Biolabs) were used for recovering single-stranded DNA. .. Double-stranded DNA was generated from the enriched DNA fragments via polymerase chain reaction (PCR) using SNX primers.

Sonication:

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: Sonication was performed three times at 50% power for 5 sec with 30-sec intervals at room temperature. .. The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences).

Injection:

Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6
Article Snippet: Three hours after injection, mice were killed with an overdose of sodium pentobarbital (Nembutal), and blood was collected via cardiac puncture into Eppindorf tubes and allowed to clot at room temperature for 1 h. Blood was centrifuged at 8000 × g for 10 min at 4°C, and the serum was aliquoted and stored at −80°C until use. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C.

Binding Assay:

Article Title: Association of UHRF1 with H3K9 methylation directs the maintenance of DNA methylation
Article Snippet: Heat maps were generated using Java TreeView. .. A 50 µL slurry of streptavidin magnetic beads (NEB) was equilibrated in binding buffer containing 50 mM Tris-HCl, pH 8.0, 300 mM NaCl, and 0.1% NP-40 before being saturated with 1 nmole biotinylated peptide for 1 hour at 4°C with rotation. .. Unbound peptide was washed with binding buffer, and 100 pmoles of protein in binding buffer supplemented with 0.5% bovine serum albumin (BSA) (w/v) was incubated for 3 hours at 4°C with rotation.

Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6
Article Snippet: ELISAs for IL-6, IFN γ , and IL-1 β (R & D Systems) were preformed according to the manufacturer’s instructions. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C. .. After washing the beads thoroughly with PBS, mouse serum was diluted 1:20 in PBS and incubated with the beads for 4 h at room temperature.

Pull Down Assay:

Article Title: Lactose repressor hinge domain independently binds DNA
Article Snippet: Paragraph title: Magnetic bead DNA pull‐down assay ... Streptavidin magnetic beads (New England BioLabs) were placed in a magnetic tube rack and the supernatant removed.

Fluorescence:

Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay
Article Snippet: Fluorescence spectra of 500 nM solutions of either TEM8-mCit, PAE733C*AF546 , or mixtures of PAE733C*AF546 and TEM8-mCit were also acquired in HEPES buffered saline + Tween-20 (HiHBST; 50 mM HEPES pH 7.4, 150 mM NaCl, 2 mM MgCl2 , 0.1% Tween-20) following a 1 hr incubation at room temperature. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of streptavidin functionalized magnetic beads (New England Biolabs) for an additional 15 minutes.

Magnetic Beads:

Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics
Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with streptavidin magnetic beads (New England Biolabs) on a rotator at 4°C overnight. .. After incubation, samples were washed five times with IP buffer and then processed for and analyzed by mass spectrometry to identify eluted proteins.

Article Title: Lactose repressor hinge domain independently binds DNA
Article Snippet: Fluorescence shift in the presence of IPTG (100 µM ) demonstrated folded core domain for the purified His6 ‐tagged proteins. .. Streptavidin magnetic beads (New England BioLabs) were placed in a magnetic tube rack and the supernatant removed. .. Beads (25 µL of original suspension per sample) were washed three times in an equal volume of Buffer 1 containing 10 mM Tris‐HCl, pH 7.5, 1 mM EDTA, 0.5 M NaCl.

Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis
Article Snippet: For each assay, 40μg of genomic DNA was predigested with RsaI and HinfI and subsequently subjected to an annealing reaction to a biotin-labeled C-rich oligo (AATCCC)6 (Metabion, Martinsried, Germany) at decreasing temperatures of 80°C, 65°C, 55°C, 45°C, 35°C for 30min each in 1xSSC/0.1% Triton X-100. .. After the annealing reaction streptavidin-coated magnetic beads (20μl of a 10mg/ml stock solution) (New England Biolabs, Beverly, MA, USA) that were pre-washed (1xSSC/1% Triton X-100, coated with 5x Denhardt’s for 30min at RT) and resuspended in 1x SSC were added to the reaction mixture. .. To recover telomeres, beads were gently washed twice in ice cold 1x SSC/1% Triton X-100.

Article Title: An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis
Article Snippet: Unbound biotin was removed by chloroform extraction using phase lock gel (5 Prime), and the RNA was precipitated from the aqueous phase by adding one-tenth volume of 5 M NaCl and 1.1 volumes of isopropanol. .. Biotinylated RNA was then was separated from unlabeled RNA using streptavidin-coated magnetic beads (New England Biolabs). .. Biotinylated RNA (75 to 100 µg) was added to the beads, and the solution was incubated for 20 min at room temperature.

Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes
Article Snippet: The excess biotin was removed using precipitation with 3 M sodium acetate and absolute ethanol, and resuspending the probes in 100 μl of bidistilled water. .. To isolate SSR-containing fragments, the probes were attached to Streptavidin magnetic beads (New England Biolabs, Ipswitch, MA) according to the manufacturer’s instructions. .. The product of the enrichment-PCR was denatured at 95°C for 5 min and quickly chilled on ice.

Article Title: Dopamine Neuron Stimulating Actions of a GDNF Propeptide
Article Snippet: F344 substantia nigra was homogenized in homogenization buffer and cytosolic fraction (supernatant) collected after 30 minutes at 100,000 g. 50 µg of bDNSP-11 was incubated with fraction for 15 minutes on ice. .. Sample was added to streptavidin magnetic beads, pelleted, and washed four times in homogenization buffer. .. Bound proteins were eluted by Solubilization/Rehydration Solution (7 M Urea, 2 M Thiourea, 50 mM DTT, 4% CHAPS, 1% NP-40, 0.2% Carrier ampholytes, 0.0002% Bromophenol blue), and analyzed by 2D-PAGE and later identified by MALDI-TOF MS/MS ( ). (0.41 MB DOC) Click here for additional data file.

Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
Article Snippet: After 10 min incubation at 70°C, the mRNA/primer mixture was transferred to 45°C and mixed with 5× first-strand buffer, 25 mM MgCl2 , 10 mM deoxyribonucleotide triphosphate (dNTP) mix, and ImProm-II™ Reverse Transcriptase (Promega, Madison, WI, USA) at the total volume of 40 μl. .. The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed streptavidin-coated magnetic beads (1.1 mg/ml; New England BioLabs, Ipswich, MA, USA). .. The mixed suspension was then incubated at room temperature for 60 min with constant rotating and then inserted into a magnet for 2 min before removing the supernatant.

Article Title: Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB
Article Snippet: Each mole of His-HpaII was found to contain 8.4 mole of biotin. .. A 20 μl aliquot of streptavidin magnetic beads (New England Biolabs) was washed with once with 200 μl Buffer A (10 mM Tris pH 8.0, 50 mM NaCl, 10 mM CaCl2 , 0.01% Tween 20) and resuspended in 50 μl of Buffer A containing 500 ng of biotinylated-His-HpaII. .. After pipette mixing to allow the His-HpaII to bind to the beads, the His-HpaII-beads (“HpaII-beads” for simplicity) were washed again with Buffer A. Enrichments were performed either in 1.7 ml microcentrifuge tubes or in a 96-well plate.

Article Title: Removing the needle from the haystack: Enrichment of Wolbachia endosymbiont transcripts from host nematode RNA by Cappable-seq™
Article Snippet: 50 μL of eluted RNA was mixed with 50 μL of 10 mM Tris-HCl, pH 7.5, 500 mM NaCl, 1 mM EDTA (wash Buffer A). .. This mix was added to 100 μL of hydrophilic streptavidin magnetic beads (New England Biolabs) previously washed twice with wash Buffer B (10 mM Tris-HCl, pH 7.5, 50 mM NaCl) and twice with wash Buffer A (100 μL/wash). .. The RNA-bead mixture was incubated for 20 min at room temperature with occasional resuspension.

Isolation:

Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
Article Snippet: The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed streptavidin-coated magnetic beads (1.1 mg/ml; New England BioLabs, Ipswich, MA, USA). .. The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed streptavidin-coated magnetic beads (1.1 mg/ml; New England BioLabs, Ipswich, MA, USA).

Size-exclusion Chromatography:

Labeling:

Purification:

Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing
Article Snippet: Paragraph title: Protein–RNA purification ... The eluate was neutralized with 1 M Tris base and loaded onto streptavidin magnetic beads (New England Biolabs S1420S) from a 200 μL slurry that was pre-equilibrated in buffer 3 (8 M urea, 200 mM NaCl, 0.2% SDS, 100 mM Tris-HCl at pH 8.0, and EDTA-free protease inhibitor mix for His-Tag sequences).

Blocking Assay:

Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6
Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to streptavidin magnetic beads (NEB, Ipswich, MA) overnight at 4°C.

Activated Clotting Time Assay:

Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes
Article Snippet: The oligonucleotides were mixed as suggested by Glenn and Schable [ ]: mix 2 ((AG)12 , (TG)12 , (AAC)6 , (AAG)8 , (AAT)12 , (ACT)12 , (ATC)8 ); mix 3 ((AAAC)6 , (AAAG)6 , (AATC)6 , (AATG)6 , (ACAG)6 , (ACCT)6 , (ACTC)6 , (ACTG)6 ); and mix 4 ((AAAT)8 , (AACT)8 , (AAGT)8 , (ACAT)8 , (AGAT)8 ). .. To isolate SSR-containing fragments, the probes were attached to Streptavidin magnetic beads (New England Biolabs, Ipswitch, MA) according to the manufacturer’s instructions.

Mouse Assay:

SDS Page:

Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
Article Snippet: After incubation on ice for 30 min, 50 μl of streptavidin magnetic beads (New England Biolabs) was added, and the mixture was incubated at room temperature for 30 min. .. The beads were washed twice with 100 μl binding buffer.

Plasmid Preparation:

Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae)
Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Streptavidin-coated magnetic beads (New England Biolabs) were used for recovering single-stranded DNA. .. Double-stranded DNA was generated from the enriched DNA fragments via polymerase chain reaction (PCR) using SNX primers.

Real-time Polymerase Chain Reaction:

Article Title: Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB
Article Snippet: A 20 μl aliquot of streptavidin magnetic beads (New England Biolabs) was washed with once with 200 μl Buffer A (10 mM Tris pH 8.0, 50 mM NaCl, 10 mM CaCl2 , 0.01% Tween 20) and resuspended in 50 μl of Buffer A containing 500 ng of biotinylated-His-HpaII. .. A 20 μl aliquot of streptavidin magnetic beads (New England Biolabs) was washed with once with 200 μl Buffer A (10 mM Tris pH 8.0, 50 mM NaCl, 10 mM CaCl2 , 0.01% Tween 20) and resuspended in 50 μl of Buffer A containing 500 ng of biotinylated-His-HpaII.

Homogenization:

Immunoprecipitation:

Marker:

Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
Article Snippet: After incubation on ice for 30 min, 50 μl of streptavidin magnetic beads (New England Biolabs) was added, and the mixture was incubated at room temperature for 30 min. .. After the bound NS5A was solubilized in 1× SDS sample buffer at 95°C for 10 min, the beads were spun down and the supernatant was analyzed by SDS-PAGE on a 12.5% gel.

S1420