streptavidin magnetic beads (New England Biolabs)

99
Name:
Streptavidin Magnetic Beads
Description:
Streptavidin Magnetic Beads 5 ml
Catalog Number:
S1420S
Price:
303
Size:
5 ml
Category:
Magnetic Separation Equipment
Score:
85
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New England Biolabs
streptavidin magnetic beads

Streptavidin Magnetic Beads 5 ml
https://www.bioz.com/result/streptavidin magnetic beads/product/New England Biolabs
Average 99 stars, based on 93 article reviews
Price from $9.99 to $1999.99

Streptavidin Magnetic Beads 5 ml
https://www.bioz.com/result/streptavidin magnetic beads/product/New England Biolabs
Average 99 stars, based on 93 article reviews
Price from $9.99 to $1999.99
streptavidin magnetic beads - by Bioz Stars,
2019-12
99/100 stars
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Clone Assay:Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: MARCH5 wild-type and MARCH5H43W coding sequences were cloned into MCS-BirA(R118G)-HA vector ( , ; a gift from Kyle Roux; Addgene plasmid 36047) by using Nhe I and Bam HI restriction sites. .. Samples were incubated with Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], Centrifugation:Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Cell lysates were cleared by centrifugation at 40,000 rpm in a Beckman L-80 ultracentrifuge at room temperature for 30 min using Ti 70.1 rotor. .. The eluate was neutralized with 1 M Tris base and loaded onto Amplification:Article Title: Microdroplet-based PCR amplification for large scale targeted sequencing Article Snippet: 110 μL of Synthesized:Article Title: An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis Article Snippet: Paragraph title: Metabolic Labeling of Newly Synthesized RNA ... Biotinylated RNA was then was separated from unlabeled RNA using Construct:Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: The constructs MARCH5-BirA(R118G)-HA, MARCH5H43W -BirA(R118G)-HA, and the BirA(R118G)-HA (as a control) were transfected into cells using Lipofectamine 2000 transfection reagent (Life Technologies). .. Samples were incubated with Enzyme-linked Immunosorbent Assay:Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Incubation:Article Title: Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1 Article Snippet: The streptavidin magnetic beads were resuspended in 200 µl of protease K buffer follow by the addition of Protease K (Ambion) to the final concentration of 1.2 mg/ml. .. After incubation at 55°C for 30 minutes, the supernatant was transferred to a new tube and another 200 µl of protease K buffer was added to the Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: In a typical experiment, 1 μM synthetic rU7rC4-biotin was mixed with 1 μM purified NS5A protein in 50 μl binding buffer containing 50 mM HEPES (pH 7.5), 5 mM MgCl2 , and 10 mM ΒME. .. After incubation on ice for 30 min, 50 μl of Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Article Title: Removing the needle from the haystack: Enrichment of Wolbachia endosymbiont transcripts from host nematode RNA by Cappable-seq™ Article Snippet: This mix was added to 100 μL of hydrophilic Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Cleared lysates were pooled and incubated with Ni-NTA agarose (Qiagen, 500 μL slurry pre-equilibrated in buffer 1) for 3 h at room temperature. .. The eluate was neutralized with 1 M Tris base and loaded onto Article Title: Microdroplet-based PCR amplification for large scale targeted sequencing Article Snippet: 110 μL of Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses Article Snippet: The combined DNA lysate mixture was incubated at 37 °C for 1 h and then quenched by addition of an equal volume of quenching/bead binding buffer (40 mM Tris⋅HCl, pH 7.6, 200 mM NaCl, 2 mM DTT, 15% PEG 6000, 20 mM EDTA). .. Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis Article Snippet: After the annealing reaction Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: Extracted genomic DNA was digested with Bsa AI and Hin cII and ligated to SNX linkers using T4 DNA ligase ( ). .. The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: After 10 min incubation at 70°C, the mRNA/primer mixture was transferred to 45°C and mixed with 5× first-strand buffer, 25 mM MgCl2 , 10 mM deoxyribonucleotide triphosphate (dNTP) mix, and ImProm-II™ Reverse Transcriptase (Promega, Madison, WI, USA) at the total volume of 40 μl. .. The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay Article Snippet: A PA-TEM8-mCit binding assay consisted of mixing PAE733C labeled with biotin-PEG2 -maleimide (Pierce; labeled according to manufacturer protocols) with TEM8-mCit at 1 μM each in HiHBST. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of Expressing:Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses Article Snippet: Expression of each gene product candidate was confirmed by SDS/PAGE analysis. .. Western Blot:Article Title: Association of UHRF1 with H3K9 methylation directs the maintenance of DNA methylation Article Snippet: A 50 µL slurry of Article Title: Molecular Characterization of Zebrafish Oatp1d1 (Slco1d Article Snippet: Oatp1d1 is present in three forms: as a monomeric protein of ∼80 kDa, as a dimeric form of ∼150 kDa, and as a tetramer or higher order oligomer that appears at ∼250 kDa in Western blots from the total cell lysate fraction ( A ). .. However, when the cell membrane fraction was isolated through the cell surface biotinylation, followed by binding of membrane fraction to the Transformation Assay:Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Hybridization:Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes Article Snippet: To isolate SSR-containing fragments, the probes were attached to Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: Extracted genomic DNA was digested with Bsa AI and Hin cII and ligated to SNX linkers using T4 DNA ligase ( ). .. The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], Transfection:Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with Immunoprecipitation:Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: ELISAs for IL-6, IFN γ , and IL-1 β (R & D Systems) were preformed according to the manufacturer’s instructions. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Protease Inhibitor:Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Proteins were eluted in 8 mL of buffer 2 (8 M urea, 200 mM NaCl, 2% SDS, 50 mM sodium phosphate, 10 mM EDTA, 100 mM Tris-HCl at pH 4.3, and EDTA-free protease inhibitor mix for His-Tag sequences [RPI]). .. The eluate was neutralized with 1 M Tris base and loaded onto Cell Culture:Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Proximity Assay:Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: Paragraph title: BioID proximity assay ... Samples were incubated with Polymerase Chain Reaction:Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes Article Snippet: To isolate SSR-containing fragments, the probes were attached to Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], Sonication:Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Sonication was performed three times at 50% power for 5 sec with 30-sec intervals at room temperature. .. The eluate was neutralized with 1 M Tris base and loaded onto Injection:Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: Three hours after injection, mice were killed with an overdose of sodium pentobarbital (Nembutal), and blood was collected via cardiac puncture into Eppindorf tubes and allowed to clot at room temperature for 1 h. Blood was centrifuged at 8000 × g for 10 min at 4°C, and the serum was aliquoted and stored at −80°C until use. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Binding Assay:Article Title: Association of UHRF1 with H3K9 methylation directs the maintenance of DNA methylation Article Snippet: Heat maps were generated using Java TreeView. .. A 50 µL slurry of Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: In a typical experiment, 1 μM synthetic rU7rC4-biotin was mixed with 1 μM purified NS5A protein in 50 μl binding buffer containing 50 mM HEPES (pH 7.5), 5 mM MgCl2 , and 10 mM ΒME. .. After incubation on ice for 30 min, 50 μl of Article Title: Molecular Characterization of Zebrafish Oatp1d1 (Slco1d Article Snippet: Oatp1d1 is present in three forms: as a monomeric protein of ∼80 kDa, as a dimeric form of ∼150 kDa, and as a tetramer or higher order oligomer that appears at ∼250 kDa in Western blots from the total cell lysate fraction ( A ). .. However, when the cell membrane fraction was isolated through the cell surface biotinylation, followed by binding of membrane fraction to the Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: ELISAs for IL-6, IFN γ , and IL-1 β (R & D Systems) were preformed according to the manufacturer’s instructions. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Article Title: Microdroplet-based PCR amplification for large scale targeted sequencing Article Snippet: 110 μL of Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses Article Snippet: The combined DNA lysate mixture was incubated at 37 °C for 1 h and then quenched by addition of an equal volume of quenching/bead binding buffer (40 mM Tris⋅HCl, pH 7.6, 200 mM NaCl, 2 mM DTT, 15% PEG 6000, 20 mM EDTA). .. Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay Article Snippet: A PA-TEM8-mCit binding assay consisted of mixing PAE733C labeled with biotin-PEG2 -maleimide (Pierce; labeled according to manufacturer protocols) with TEM8-mCit at 1 μM each in HiHBST. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of Pull Down Assay:Article Title: Lactose repressor hinge domain independently binds DNA Article Snippet: Paragraph title: Magnetic bead DNA pull‐down assay ... Fluorescence:Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay Article Snippet: Fluorescence spectra of 500 nM solutions of either TEM8-mCit, PAE733C*AF546 , or mixtures of PAE733C*AF546 and TEM8-mCit were also acquired in HEPES buffered saline + Tween-20 (HiHBST; 50 mM HEPES pH 7.4, 150 mM NaCl, 2 mM MgCl2 , 0.1% Tween-20) following a 1 hr incubation at room temperature. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of Magnetic Beads:Article Title: Association of UHRF1 with H3K9 methylation directs the maintenance of DNA methylation Article Snippet: Heat maps were generated using Java TreeView. .. A 50 µL slurry of Article Title: Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-Poly(A) Termination Pathway: Nrd1, Nab3, and Sen1 Article Snippet: The streptavidin magnetic beads were resuspended in 200 µl of protease K buffer follow by the addition of Protease K (Ambion) to the final concentration of 1.2 mg/ml. .. After incubation at 55°C for 30 minutes, the supernatant was transferred to a new tube and another 200 µl of protease K buffer was added to the Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: At the time of transfections, cells were treated with 50 mM biotin for 24 h and then lysed in IP buffer for 30 min at 4°C. .. Samples were incubated with Article Title: Lactose repressor hinge domain independently binds DNA Article Snippet: Fluorescence shift in the presence of IPTG (100 µM ) demonstrated folded core domain for the purified His6 ‐tagged proteins. .. Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: In a typical experiment, 1 μM synthetic rU7rC4-biotin was mixed with 1 μM purified NS5A protein in 50 μl binding buffer containing 50 mM HEPES (pH 7.5), 5 mM MgCl2 , and 10 mM ΒME. .. After incubation on ice for 30 min, 50 μl of Article Title: Molecular Characterization of Zebrafish Oatp1d1 (Slco1d Article Snippet: Oatp1d1 is present in three forms: as a monomeric protein of ∼80 kDa, as a dimeric form of ∼150 kDa, and as a tetramer or higher order oligomer that appears at ∼250 kDa in Western blots from the total cell lysate fraction ( A ). .. However, when the cell membrane fraction was isolated through the cell surface biotinylation, followed by binding of membrane fraction to the Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: ELISAs for IL-6, IFN γ , and IL-1 β (R & D Systems) were preformed according to the manufacturer’s instructions. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes Article Snippet: The excess biotin was removed using precipitation with 3 M sodium acetate and absolute ethanol, and resuspending the probes in 100 μl of bidistilled water. .. To isolate SSR-containing fragments, the probes were attached to Article Title: Dopamine Neuron Stimulating Actions of a GDNF Propeptide Article Snippet: F344 substantia nigra was homogenized in homogenization buffer and cytosolic fraction (supernatant) collected after 30 minutes at 100,000 g. 50 µg of bDNSP-11 was incubated with fraction for 15 minutes on ice. .. Sample was added to Article Title: Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB Article Snippet: Each mole of His-HpaII was found to contain 8.4 mole of biotin. .. A 20 μl aliquot of Article Title: Removing the needle from the haystack: Enrichment of Wolbachia endosymbiont transcripts from host nematode RNA by Cappable-seq™ Article Snippet: 50 μL of eluted RNA was mixed with 50 μL of 10 mM Tris-HCl, pH 7.5, 500 mM NaCl, 1 mM EDTA (wash Buffer A). .. This mix was added to 100 μL of hydrophilic Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Proteins were eluted in 8 mL of buffer 2 (8 M urea, 200 mM NaCl, 2% SDS, 50 mM sodium phosphate, 10 mM EDTA, 100 mM Tris-HCl at pH 4.3, and EDTA-free protease inhibitor mix for His-Tag sequences [RPI]). .. The eluate was neutralized with 1 M Tris base and loaded onto Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses Article Snippet: The combined DNA lysate mixture was incubated at 37 °C for 1 h and then quenched by addition of an equal volume of quenching/bead binding buffer (40 mM Tris⋅HCl, pH 7.6, 200 mM NaCl, 2 mM DTT, 15% PEG 6000, 20 mM EDTA). .. Article Title: Detection of circular telomeric DNA without 2-D gel electrophoresis Article Snippet: For each assay, 40μg of genomic DNA was predigested with RsaI and HinfI and subsequently subjected to an annealing reaction to a biotin-labeled C-rich oligo (AATCCC)6 (Metabion, Martinsried, Germany) at decreasing temperatures of 80°C, 65°C, 55°C, 45°C, 35°C for 30min each in 1xSSC/0.1% Triton X-100. .. After the annealing reaction Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: Extracted genomic DNA was digested with Bsa AI and Hin cII and ligated to SNX linkers using T4 DNA ligase ( ). .. The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Article Title: An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis Article Snippet: Unbound biotin was removed by chloroform extraction using phase lock gel (5 Prime), and the RNA was precipitated from the aqueous phase by adding one-tenth volume of 5 M NaCl and 1.1 volumes of isopropanol. .. Biotinylated RNA was then was separated from unlabeled RNA using Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: After 10 min incubation at 70°C, the mRNA/primer mixture was transferred to 45°C and mixed with 5× first-strand buffer, 25 mM MgCl2 , 10 mM deoxyribonucleotide triphosphate (dNTP) mix, and ImProm-II™ Reverse Transcriptase (Promega, Madison, WI, USA) at the total volume of 40 μl. .. The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: It can be performed in most conventional laboratories without requiring the purchasing of expensive kits and/or pieces of equipment. .. The highlights of this protocol include (a) its great success for driver preparation and subtractive hybridization using just tiny amounts of mRNAs , when comparing it to most current protocols that require huge amount of mRNAs for the same purpose [ ]. (b) As compared with previously reported protocols that apply paramagnetic oligo (dT) beads as solid base for subtractive hybridization [ , ], Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay Article Snippet: A PA-TEM8-mCit binding assay consisted of mixing PAE733C labeled with biotin-PEG2 -maleimide (Pierce; labeled according to manufacturer protocols) with TEM8-mCit at 1 μM each in HiHBST. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of Isolation:Article Title: Molecular Characterization of Zebrafish Oatp1d1 (Slco1d Article Snippet: Oatp1d1 is present in three forms: as a monomeric protein of ∼80 kDa, as a dimeric form of ∼150 kDa, and as a tetramer or higher order oligomer that appears at ∼250 kDa in Western blots from the total cell lysate fraction ( A ). .. However, when the cell membrane fraction was isolated through the cell surface biotinylation, followed by binding of membrane fraction to the Article Title: A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf Article Snippet: The reaction volume was increased to 150 μl by adding Tris–EDTA (TE) buffer before mixing it with 160 μl of TE-washed Size-exclusion Chromatography:Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Sonication was performed three times at 50% power for 5 sec with 30-sec intervals at room temperature. .. The eluate was neutralized with 1 M Tris base and loaded onto Labeling:Article Title: An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis Article Snippet: Paragraph title: Metabolic Labeling of Newly Synthesized RNA ... Biotinylated RNA was then was separated from unlabeled RNA using Article Title: Identification of small molecules that inhibit the interaction of TEM8 with anthrax protective antigen using a FRET assay Article Snippet: A PA-TEM8-mCit binding assay consisted of mixing PAE733C labeled with biotin-PEG2 -maleimide (Pierce; labeled according to manufacturer protocols) with TEM8-mCit at 1 μM each in HiHBST. .. The mixture was incubated for 1 hr at room temperature, then with 2 μl of Mouse Assay:Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: Three hours after injection, mice were killed with an overdose of sodium pentobarbital (Nembutal), and blood was collected via cardiac puncture into Eppindorf tubes and allowed to clot at room temperature for 1 h. Blood was centrifuged at 8000 × g for 10 min at 4°C, and the serum was aliquoted and stored at −80°C until use. .. For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Blocking Assay:Article Title: Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 Article Snippet: For immunoprecipitation of anti-cytokine antibodies, a biotinylated, anti-rat antibody that was preabsorbed with mouse serum to prevent binding to mouse antibodies (Vector Laboratories, Burlingame, CA) was conjugated to Activated Clotting Time Assay:Article Title: Development of genomic SSR markers for fingerprinting lettuce (Lactuca sativa L.) cultivars and mapping genes Article Snippet: The oligonucleotides were mixed as suggested by Glenn and Schable [ ]: mix 2 ((AG)12 , (TG)12 , (AAC)6 , (AAG)8 , (AAT)12 , (ACT)12 , (ATC)8 ); mix 3 ((AAAC)6 , (AAAG)6 , (AATC)6 , (AATG)6 , (ACAG)6 , (ACCT)6 , (ACTC)6 , (ACTG)6 ); and mix 4 ((AAAT)8 , (AACT)8 , (AAGT)8 , (ACAT)8 , (AGAT)8 ). .. To isolate SSR-containing fragments, the probes were attached to Purification:Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: In a typical experiment, 1 μM synthetic rU7rC4-biotin was mixed with 1 μM purified NS5A protein in 50 μl binding buffer containing 50 mM HEPES (pH 7.5), 5 mM MgCl2 , and 10 mM ΒME. .. After incubation on ice for 30 min, 50 μl of Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: Paragraph title: Protein–RNA purification ... The eluate was neutralized with 1 M Tris base and loaded onto SDS Page:Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: After incubation on ice for 30 min, 50 μl of Article Title: Identification and biosynthesis of thymidine hypermodifications in the genomic DNA of widespread bacterial viruses Article Snippet: Expression of each gene product candidate was confirmed by SDS/PAGE analysis. .. Plasmid Preparation:Article Title: Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5–dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics Article Snippet: MARCH5 wild-type and MARCH5H43W coding sequences were cloned into MCS-BirA(R118G)-HA vector ( , ; a gift from Kyle Roux; Addgene plasmid 36047) by using Nhe I and Bam HI restriction sites. .. Samples were incubated with Article Title: Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae) Article Snippet: The ligated fragments were enriched through hybridisation with biotinylated dimeric and trimeric nucleotide repeats (CT8 , TC8 , TA8 , TG8 , GC8 , CTT8 , ATA8 and GAA8 ) in three reactions, with incubation at 97 °C for 10 min, followed by 56 °C for 30 min. Real-time Polymerase Chain Reaction:Article Title: Epigenetic Segregation of Microbial Genomes from Complex Samples Using Restriction Endonucleases HpaII and McrB Article Snippet: A 20 μl aliquot of Homogenization:Article Title: Dopamine Neuron Stimulating Actions of a GDNF Propeptide Article Snippet: F344 substantia nigra was homogenized in homogenization buffer and cytosolic fraction (supernatant) collected after 30 minutes at 100,000 g. 50 µg of bDNSP-11 was incubated with fraction for 15 minutes on ice. .. Sample was added to Concentration Assay:Article Title: Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing Article Snippet: The eluate was neutralized with 1 M Tris base and loaded onto Marker:Article Title: Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins Article Snippet: After incubation on ice for 30 min, 50 μl of |