Journal: Communications Biology
Article Title: Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention
Figure Lengend Snippet: BDNFpro expression in cortical astrocytes. a Schematic representation of proBDNF precursor and cleaved BDNFpro domain. αBDNFpro antibody recognizes the furin cleavage site of the prodomain. Western blotting probing recombinant mBDNF, BDNFpro, and proBDNF CR with αBDNFpro and αmBDNF antibodies. b Cortical slices from control mice injected with AAV-GFAP-GFP virus were recorded and fixed 10 min after TBS for immunostaining. z-stack reconstruction shows astrocytes labeled by GFP. Magnification of a single stack from a region of interest (ROI) shows BDNFpro immunoreactivity and BDNFpro/GFP colocalization signal of one GFP-astrocyte delimited by an approximate territory (white dashed). Scale bars: 10 µm. c z-stack reconstruction of BDNFpro/GFP colocalization signals in astrocytes from baseline- and TBS-slices from control mice. The insets show GFP signal. BDNFpro/GFP colocalization was quantified in the whole cell and branches using Mander’s overlap. *** p
Article Snippet: In some experiments, recombinant BDNFpro (10 ng/ml; Alomone Labs, Cat#B-245), BDNFproVal/Met (10 ng/ml; Alomone Labs, Cat#B-445), mBDNF (10 ng/ml; Laboratory of Antonino Cattaneo, SNS, Pisa, Italy), and proBDNFCR (20 ng/ml; Laboratory of Antonino Cattaneo, SNS, Pisa, Italy) were perfused into a recording chamber.
Techniques: Expressing, Western Blot, Recombinant, Mouse Assay, Injection, Immunostaining, Labeling