Journal: bioRxiv

Article Title: Cholinergic Modulation Shifts the Response of CA1 Pyramidal Cells to Depolarizing Ramps via TRPM4 Channels with Implications for Place Field Firing

doi: 10.1101/2022.10.24.513511

Figure Lengend Snippet: Cholinergic-associated shift in the timing of firing on ramp is mediated by TRP channels, but notably not TRPC channels. A. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (A1), CCh (2 μM, A2), and CCh + FFA (100 μM, A3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (A4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). B. Same as in A, but for dendritic injection and recordings. Summary data (B4) of the adaptation index for control, CCh, and CCh + FFA (n = 9). Open circles connected by dashed lines represent indices for individual cells, over all three treatments. Black squares with error bars represent group averages ± SEM. C. Voltage traces recorded in the soma for a two second triangular current ramp injected in the soma in control (C1), CCh (2 μM, C2), and CCh + SKF96365 (50 μM, C3) applied subsequently to the same cell. Current traces and instantaneous frequency plots are just below voltage traces. Red dotted line marks the middle of the ramp. Summary data (C4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). D. Same as in C, but for dendritic injection and recordings. Summary data (D4) of the adaptation index for control, CCh, and CCh + SKF96365 (n = 8). *** p < 0.0005, ** p = 0.001, * p = 0.02, n.s. not significant.

Article Snippet: Carbachol, 9-phenanthrol, and CBA were purchased from Tocris, CGP55845 from Abcam (Cambridge, MA), DL-APV from HelloBio (Princeton, NJ), NBQX, Gabazine, and SKF96365 from Alomone Labs (Jerusalem, Israel), and acetylcholine, flufenamic acid, and K4-BAPTA from Sigma (St. Louis, MO).

Techniques: Injection

Journal: Biology of Reproduction

Article Title: The role of alkalinization-induced Ca 2+ influx in sperm motility activation of a viviparous fish Redtail Splitfin ( Xenotoca eiseni )

doi: 10.1093/biolre/ioy150

Figure Lengend Snippet: Intracellular Ca2+ increase is dependent on extracellular sources and the increases were not inhibited by major Ca2+ channel blockers. (A) Thapsigargin (TG, 1 μM) was added to bundle suspensions with or without 1 mM CaCl2 to deplete intracellular Ca2+ stores prior increase of pH to 8.5. (B) Addition of 1 mM CaCl2 to the extracellular buffer increased Ca2+ influx after stimulation with pH 8.5 under Ca2+-free conditions. (C) Bundles were pretreated the respective blockers (CdCl2 (200 μM), NiCl2 (300 μM), ruthenium red (10 μM), nimodipine (30 μM), verapamil (30 μM), mibefradil (40 μM), NNC 55–0396 (10 μM), GdCl3 (100 μM), SKF-96365 (100 μM), methoxyverapamil (D600 30 μM), ω-Conotoxin MVIIC (2 μM), bepridil (50 μM), or 2-APB (300 μM) for 20 min prior to stimulation by extracellular exposure to 1 mM CaCl2 stimulation and pH 8.5. Bars represent means ± SEM; n = 50–150 regions of interest per male from three males. Only CdCl2 treatment (asterisk) produced a significant reduction in relation to the control group (without blocker) and blockers.

Article Snippet: Chemicals The ratiometric calcium indicator Fura-2 AM was purchased from Cayman Chemical Company ( www.caymanchem.com ), SKF-96365 and ω-Conotoxin MVIIC were from Alomone Labs ( www.alomone.com ), and Tricaine methanesulfonate (MS-222) was from Western Chemical, Inc ( www.syndel.com ).

Techniques: Produced