tbst  (Azure Biosystems)


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    Structured Review

    Azure Biosystems tbst
    Tbst, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbst/product/Azure Biosystems
    Average 90 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    tbst - by Bioz Stars, 2021-02
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Positron Emission Tomography:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Whole Genome Amplification:

    Article Title: Chitin is a functional component of the larval adhesive of barnacles
    Article Snippet: .. WGA-conjugated bands were detected by chemiluminescence using Radiance Plus reagents (Azure Biosystems) on an Azure c280 gel documentation system with variable exposure times (10–30 s). .. To verify lectin specificity, 10 μg/mL of WGA was incubated in 200 mM hapten sugar (N -acetyl-d -glucosamine; Sigma-Aldrich) for 60 min and subsequently failed to detect the protein bands.

    BAC Assay:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Mutagenesis:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Imaging:

    Article Title: Environmental stress impairs photoreceptor outer segment (POS) phagocytosis and degradation and induces autofluorescent material accumulation in hiPSC-RPE cells
    Article Snippet: .. The blots were either imaged on Odyssey Infrared Imager (Licor Biosciences) or developed using the Radiance Plus Chemiluminescence Kit and imaged on Azure C500 imaging system (Azure Biosystems). .. Of note, prior to reprobing with a different antibody, the PVDF membrane was stripped using NewBlot PVDF stripping buffer (Licor Biosciences) for 60 min at room temperature.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Sequencing:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Western Blot:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

    Recombinant:

    Article Title: Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.
    Article Snippet: .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER FuGENE6 Promega E2691 Lipofectamine 3000 Life Technologies L3000001 ECL Prime Western Blotting Detection Reagent GE/Amersham RPN2106 Radiance Plus Chemiluminescent substrate Azure Biosystems AC2103 Protein A agarose beads Thermo Fisher 15918-014 25-hydroxycholesterol Sigma Aldrich H1015-10MG CytofixTM fixation buffer BD Biosciences 554714 ProLongTM Gold antifade mountant Thermo Fisher P-36931 Dulbelcco’s Phosphate Buffered Saline (DPBS) Corning 45000-434 Gentamicin VWR Scientific 90002-262 Goat Serum Abcam ab156046 Digitonin Sigma Aldrich D141-100MG Rhodamine Phalloidin Life Technologies R415 VectaShield Vector Laboratories H-1000 DharmaFect siRNA transfection reagent Dharmacon T-2001-01 Ni-NTA-agarose resin QIAGEN 30210 Critical Commercial Assays Normalized Mate Plate TM library from HeLa S3 Clontech 630479 Q5TM Site-Directed mutagenesis kit New England Biolabs E0554S Bac-to-Bac system Invitrogen 10359016 Endo-free Maxi kit QIAGEN 12362 Matchmaker GoldTM Gal4-based yeast-two-hybrid system Clontech 630489 Experimental Models: Cell Lines HeLa VAPDKO Pietro de Camilli Lab Dong et al., 2016 HeLA ATCC CVCL_0030 Oligonucleotides OSBP1-targeting siRNA Dharmacon L-009747-00-0005 nontargeting siRNA pool Dharmacon D-001810-10-05 TFP coding sequence Allele Biotechnology ABP-FP-TCNCS10 Recombinant DNA pETDuetTM-1 Millipore 71146-3 pETTM-28a Millipore 69864 pCMV-HA Clontech 635690 pCMV-myc Clontech 635689 pFastBac ThermoFisher 10712-024 pBAD18-mTFP This study N/A pAK071 (Osbp1191-432 in pCMV-HA) This study N/A pAK072 (Osbp1191-405 in pCMV-HA) This study N/A pAK079 (Osbp1 in pCMV-HA) This study N/A pAK093 (Osbp1191-357 in pCMV-HA) This study N/A pAK095 (His-SseJ1-133 in pET-28a) This study N/A pAK098 (Osbp1191-327 in pCMV-HA) This study N/A pAK099 (Osbp1191-357 in pET-28a) This study N/A pAK105 (Osbp2285-422 in pCMV-HA) This study N/A pAK107 (His-SseJ1-133 and OSBP1191-327 in pETDuet-1) This study N/A pAK111 (SseL in pCMV-myc) This study N/A pAK153 (His-SspH2 in pET-28a) This study N/A pET28a-SseJ (His-SspH2 in pET-28a) LaRock et al., 2012 N/A (Continued on next page) e2 Cell Reports 27, 2147–2156.e1–e5, May 14, 2019. .. ContinuedREAGENT or RESOURCE SOURCE IDENTIFIER pAK188 (His-SseJ1-133 and OSBP1191-405 in pETDuet-1) This study N/A pAK192 (His-OSBP1191-405 in pET-28a) This study N/A pWSK29 Wang and Kushner, 1991 N/A pKF34 (sseJ with native promoter in pWSK29) Ohlson et al., 2008 N/A pAK221 (sseL with native promoter in pWSK29) This study N/A pAK222 (sseL with native promoter in pKF34) This study N/A pMBO110 (SifA in pCMV-myc) Ohlson et al., 2008 N/A Myc-SifB (SifB in pCMV-myc) This study N/A pMBO102 (SseJ in pCMV-myc) Ohlson et al., 2008 N/A pMBO103 (SseJ3x in pCMV-myc) Ohlson et al., 2008 N/A pDL80 (SseJF121D in pCMV-myc) LaRock et al., 2012 N/A pHis-RhoA (His-RhoA in pET-28a) This study N/A.

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    Azure Biosystems radiance hrp substrate
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