Journal: The Journal of Neuroscience

Article Title: Mature BDNF, But Not proBDNF, Reduces Excitability of Fast-Spiking Interneurons in Mouse Dentate Gyrus

doi: 10.1523/JNEUROSCI.2978-09.2009

Figure Lengend Snippet: Chronic or acute reductions in BDNF lead to increased frequency of spontaneous IPSCs in granule cells involving TrkB receptors. Whole-cell recordings of GABAergic IPSCs in granule cells of the mouse dentate gyrus. A, Spontaneous GABAA receptor-mediated IPSCs recorded in WT and BDNF+/− mice. The action-potential-driven sIPSCs recorded without TTX showed a substantial increase by 65% in BDNF+/− slices. B, Brain slices from WT mice were incubated with either a control antibody (human Fc, 0.74 μg/ml) for 4 h or TrkB-Fc (2.2 μg/ml). In TrkB-Fc, the sIPSC frequency was 200% larger than with human FC. C, Recordings of sIPSCs in control, and in a slice where K-252a (200 nm) was used to inhibit TrkB. The latter slices were incubated with K-252a for 1 h, while control slices were incubated for 1 h with vehicle. K-252a increased the sIPSC frequency by 95%. D, Histogram summarizing the frequency of sIPSCs.

Article Snippet: All drugs and chemicals were from Sigma-Aldrich except for TTX and cleavage-resistant proBDNF (Alomone), and recombinant human TrkB-Fc chimera (#688-TK) and recombinant human IgG1-Fc (#110-HG) (R&D Systems).

Techniques: Incubation