rnase (Worthington Biochemical)
Worthington Biochemical is a verified supplier 
Worthington Biochemical manufactures this product 
92
|
Name:
Ribonuclease A
Description:
A chromatographically purified lyophilized powder
Catalog Number:
LS003431
Price:
75
Source:
Bovine Pancreas
Size:
200 mg
Cas Number:
9001.99.4
|
Buy from Supplier |
Structured Review
Worthington Biochemical
rnase
A chromatographically purified lyophilized powder
https://www.bioz.com/result/rnase/product/Worthington Biochemical
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
A chromatographically purified lyophilized powder
https://www.bioz.com/result/rnase/product/Worthington Biochemical
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rnase - by Bioz Stars,
2021-10
92/100 stars
Images
1) Product Images from "Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts"
Article Title: Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
Journal: Arthritis Research & Therapy
doi: 10.1186/s13075-018-1689-6
Figure Legend Snippet: et-1 , tlr2 and tlr3 expression levels in fibroblasts stimulated with SSc-ICs or NHS-ICs pretreated with DNase/RNase. SSc-ICs treated with DNase I (20 KU/ml) or RNase (8 μg/ml) and then added to fibroblast cultures. a ATA-ICs, ACA-ICs and anti-Th/To-ICs on et-1 ; b ATA-ICs, ACA-ICs, ARA-ICs and anti-Th/To-ICs on tlr2 ; c ATA-ICs and anti-Th/To-ICs on ifn-α ; d ATA-ICs, ACA-ICs, ARA-ICs and anti-Th/To-ICs on tlr3 . * p
Techniques Used: Expressing, Acetylene Reduction Assay
2) Product Images from "Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts"
Article Title: Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
Journal: Arthritis Research & Therapy
doi: 10.1186/s13075-018-1689-6
Figure Legend Snippet: et-1 , tlr2 and tlr3 expression levels in fibroblasts stimulated with SSc-ICs or NHS-ICs pretreated with DNase/RNase. SSc-ICs treated with DNase I (20 KU/ml) or RNase (8 μg/ml) and then added to fibroblast cultures. a ATA-ICs, ACA-ICs and anti-Th/To-ICs on et-1 ; b ATA-ICs, ACA-ICs, ARA-ICs and anti-Th/To-ICs on tlr2 ; c ATA-ICs and anti-Th/To-ICs on ifn-α ; d ATA-ICs, ACA-ICs, ARA-ICs and anti-Th/To-ICs on tlr3 . * p
Techniques Used: Expressing, Acetylene Reduction Assay
Related Articles
Purification:Article Title: Differential recognition of oligomannose isomers by glycan-binding proteins involved in innate and adaptive immunity Article Snippet: .. Purified bovine Incubation:Article Title: Isolation and Characterization of Chemo-Resistant Stem Cells from a Mouse Model of Hereditary Non-Polyposis Colon Cancer Article Snippet: .. Briefly, the cell suspension was fixed in ice cold 80% EtOH for 1 h, incubated with Article Title: Plasma cell proliferative index is an independent predictor of progression in smoldering multiple myeloma Article Snippet: .. This was followed by the wash step and the incubation in the 1000 units/mL Article Title: Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts Article Snippet: .. DNase and Staining:Article Title: Isolation and Characterization of Chemo-Resistant Stem Cells from a Mouse Model of Hereditary Non-Polyposis Colon Cancer Article Snippet: .. Briefly, the cell suspension was fixed in ice cold 80% EtOH for 1 h, incubated with other:Article Title: Potential of Soluble Decellularized Extracellular Matrix for Musculoskeletal Tissue Engineering – Comparison of Various Mesenchymal Tissues Article Snippet: Followed by three washes in PBS at 4°C for 30 min each, pieces of tissues were transferred to 40 ml of Hanks Buffered Salt Solution (HBSS, Thermo Fisher Scientific, Pittsburgh, PA, United States) supplemented with 200 U/ml DNase and 50 U/ml Centrifugation:Article Title: Discovering de novo peptide substrates for enzymes using machine learning Article Snippet: .. The cells were harvested by centrifugation at 2000 × g for 30 min, resuspended in lysis buffer (50 mM Tris-HCl buffer, pH 7.5, with 250 mM NaCl), and supplemented with 0.1 mg mL−1 lysozyme (Worthington Biochemical Corp), 5 µg mL−1 DNAse I (Sigma), and 5 µg mL−1 Article Title: Discovering de novo peptide substrates for enzymes using machine learning Article Snippet: .. The cells were harvested by centrifugation at 2000 × g for 30 min, resuspended in lysis buffer (50 mM Tris-HCl buffer, pH 7.5, with 250 mM NaCl), and supplemented with 0.1 mg mL−1 lysozyme (Worthington Biochemical Corp), 5 µg mL−1 DNAse I (Sigma), and 5 µg mL−1 Lysis:Article Title: Discovering de novo peptide substrates for enzymes using machine learning Article Snippet: .. The cells were harvested by centrifugation at 2000 × g for 30 min, resuspended in lysis buffer (50 mM Tris-HCl buffer, pH 7.5, with 250 mM NaCl), and supplemented with 0.1 mg mL−1 lysozyme (Worthington Biochemical Corp), 5 µg mL−1 DNAse I (Sigma), and 5 µg mL−1 Article Title: Discovering de novo peptide substrates for enzymes using machine learning Article Snippet: .. The cells were harvested by centrifugation at 2000 × g for 30 min, resuspended in lysis buffer (50 mM Tris-HCl buffer, pH 7.5, with 250 mM NaCl), and supplemented with 0.1 mg mL−1 lysozyme (Worthington Biochemical Corp), 5 µg mL−1 DNAse I (Sigma), and 5 µg mL−1 Labeling:Article Title: Biochemical Methods to Characterize RNA Polymerase II Elongation Complexes Article Snippet: .. Transcription buffer (50 mM HEPES-KOH pH 7.5, 100 mM KCl, 1 mM MnCl2 , 0.5 mM DTT, 10% glycerol, 0.3 mM UpG, 0.1 μg/μl BSA, 1 unit/μl RNasin) 5-Bromo-UTP (Sigma-Aldrich) Nucleotide labeling mix (4 mM ATP, 2 mM 5-Bromo-UTP, 0.05 mM CTP, 5 μl of alpha-32 P-CTP 6000 Ci/mmol, MP Biomedical) 10X DNase I buffer (100 mM MgCl2 , 50 mM CaCl2 , 100 mM Tris-HCl pH 8.0) DNase I 10 unit/μl ( Recombinant:Article Title: Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts Article Snippet: .. DNase and Reverse Transcription Polymerase Chain Reaction:Article Title: Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts Article Snippet: .. DNase and |
![Purification procedure. (a ) Strategy. Cartoon (top left): chromatin loop with nucleosomes (green circles) tethered to a polymerizing complex (oval) attached to the substructure (brown). Cells are permeabilized, in some cases a run-on performed in [ 32 P]UTP so nascent RNA can be tracked, nuclei are washed with NP40, most chromatin detached with a nuclease (here, DNase I), chromatin-depleted nuclei resuspended in NLB, and polymerizing complexes released from the substructure with caspases. After pelleting, chromatin associated with polymerizing complexes in the supernatant is degraded with DNase I, and complexes partially resolved in 2D gels (using “blue native” and “native” gels in the first and second dimensions); rough positions of complexes (and a control region, c) are shown. Finally, different regions are excised, and their content analyzed by mass spectrometry. ( b ) Recovery of [ 32 P]RNA, after including a “run-on”. Fractions correspond to those at the same level in ( a ). ( c) “Run-on” activity assayed later during fractionation (as in a , but without run-on at beginning). Different fractions, with names as in ( a ), were allowed to extend transcripts by](https://storage.googleapis.com/bioz_article_images/PMC3324775/ukmss-36353-f0001.jpg)
![LC-MS analysis of RNaseT1 digests of yeast tRNA Phe−1 . ( a ) Base peak chromatogram of the <t>RNase</t> T1 digest of the yeast tRNA Phe−1 preparation (200 fmol). Chromatography was performed as described in the ‘Materials and Methods’ section. Sixteen major oligoribonucleotide peaks, indicated by arrows with peak numbers, are assigned to the fragments of yeast tRNA Phe−1 , tRNA Phe−2 , tRNA Lys−2 , or tRNA Tyr (see Table 1 ). ( b and c ) A typical MS spectrum of the RNase T1 digest of tRNA Phe−1 ; (b) [AUUUAm 2 G > p] 2− and (c) [ACmUGmAAyWAΨUm 5 CUG > p] 3− .](https://storage.googleapis.com/bioz_article_images/PMC2790879/gkp732f4.jpg)
![CID-MS/MS spectrum of [AUUUAm 2 G] 2− derived from RNase T1 digestion of yeast tRNA Phe−1 . The doubly charged ion with m / z = 966.6 was analyzed by CID. The nucleotide sequence was verified by manual interpretation of the a- and c-type (normal text) and w - and y -type ( italic text ) product ion series as indicated in the figure. The parent ion losing methyl guanine [P−B(mG) 2− ], the parent ion losing adenine [P−B(A) 2− ], the y5 ion losing methyl guanine [y5−B(mG) 2− ], the y5 ion losing adenine [y5−B(A) 2− ], y5 2− and c5 2− were doubly charged products. All other assigned signals were singly charged products, unless indicated otherwise. The asterisks indicate hydrated or dehydrated ions of a-, c-, w- or y-type products.](https://storage.googleapis.com/bioz_article_images/PMC2790879/gkp732f5.jpg)
![Mt FRS activity. ( A ) Aminoacylation of unmodified M. tuberculosis tRNA Phe . The 3′- 32 P-labeled tRNA transcript was charged with L-Phe in the presence of purified Mt FRS. The extent of aminoacylation was analyzed according to Varshney et al. ( 37 ). Prior to polyacrylamide gel electrophoresis, tRNA and Phe-tRNA were digested with RNase T1 to yield 3′- 32 P-labeled CCACCA and CCACCA-Phe, respectively. (+) and (-) indicate the presence and the absence of the enzyme. (B–D) Synthesis of the Phe-AMP intermediate by Mt FRS in the presence of unmodified M. tuberculosis tRNA Phe ( B ), E. coli tRNA Phe ( C ) and yeast tRNA Phe ( D ). Phe-[ 32 P]AMP synthesis was monitored by thin layer chromatography. Phe-AMP and ATP denote 32 P-labeled compounds. F – full reaction; -E, -tRNA and -Phe – control reactions lacking the enzyme, tRNA or amino acid.](https://storage.googleapis.com/bioz_article_images/PMC8136816/gkab272fig1.jpg)