rna (Worthington Biochemical)


Name:
Ribonucleic Acid
Description:
Primarily ribosomal RNA Suitable substrate for ribonuclease assays
Catalog Number:
ls003451
Price:
Inquire
Size:
Bulk
Source:
Baker's Yeast
Cas Number:
63231.63.0
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Structured Review

Primarily ribosomal RNA Suitable substrate for ribonuclease assays
https://www.bioz.com/result/rna/product/Worthington Biochemical
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "Activating frataxin expression by repeat-targeted nucleic acids"
Article Title: Activating frataxin expression by repeat-targeted nucleic acids
Journal: Nature Communications
doi: 10.1038/ncomms10606

Figure Legend Snippet: Mechanism of FXN activation by repeat-targeted duplex RNAs. ( a ) RIP examining the association of Ago2 with FXN pre-mRNA after treatment with 50 nM duplex RNA and analysis by real-time PCR. An arrow marks the PCR product of FXN pre-mRNA, which was confirmed by sequencing ( Supplementary Fig. 7 ) ( b ) Anti-GAA duplex RNA with central mismatches (siGAA 9,10 mm with mismatches on both strands; 25 nM) activates FXN expression at a level similar to the analogous fully complementary duplex RNA ( n =3). siExon3 is a positive control for transfection efficiency targeting exon 3 of FXN . ( c ) ChIP for RNAP2 using four different primer sets ( n =4). ( d ) ChIP for transcription-associated histone modification markers H3K4me3, H3K9me2, H3K9me3, H3K9Ac, H3K27me3 and H4Ac ( n =4–8). ( e ) FXN mRNA stability assay. Cells were transfected with duplex RNAs siGAA or CM at 25 nM ( n =3). Actinomycin D (5 μg ml −1 ) was added with fresh media 3 days after transfection and cells were collected at the indicated time points. HPRT expression was measured for normalization. All experiments were performed in GM03816 patient-derived cells. All data are presented as mean±STDEV. * P
Techniques Used: Activation Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Sequencing, Expressing, Positive Control, Transfection, Chromatin Immunoprecipitation, Modification, Stability Assay, Derivative Assay
2) Product Images from "Recognition of 5?-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2"
Article Title: Recognition of 5?-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2
Journal: Nucleic Acids Research
doi:

Figure Legend Snippet: Role of TAR and the AUG initiation codon context in binding of eIF2. Uniformly 32 P-labeled 77 nt TAR T7 transcript (0.08 pmol, 1.25 × 10 5 c.p.m./pmol) was incubated without eIF2 or with 0.12 pmol of eIF2, in the absence or presence of 0.25–2 pmol of unlabeled TAR, TAR–LUC or LUC T7 RNA transcript as shown schematically in ( C ). Electrophoresis on a native gel was used to separate free TAR RNA from complex. The autoradiogram shows free and bound RNA ( A ). Bound RNA is quantitated in ( B ).
Techniques Used: Binding Assay, Labeling, Incubation, Electrophoresis

Figure Legend Snippet: Complex formation between HIV-1 TAR and eIF2. ( A ) Uniformly 32 P-labeled 77 nt T7 transcript (0.08 pmol, 1.25 × 10 5 c.p.m./pmol) containing TAR abutted by plasmid-derived nucleotides underlined in ( C ) was incubated without eIF2 or with 0.07, 0.2 or 0.3 pmol of eIF2. The reaction mixture was subjected to electrophoresis on a native gel to separate free TAR RNA from complexes C1 and C2. The autoradiogram is shown. ( B ) In a separate experiment, TAR RNA (0.1 pmol) and, where shown, 0.25 pmol of eIF2 were incubated in the absence or presence of unlabeled TAR RNA (0.5, 1, 2 or 4 pmol) (TAR competitor). Bovine serum albumin (BSA) served as protein control. Analysis was done as in (A).
Techniques Used: Labeling, Plasmid Preparation, Derivative Assay, Incubation, Electrophoresis

Figure Legend Snippet: HIV-1 TAR and the AUG initiation codon each contribute to affinity for eIF2. ( A ) Uniformly 32 P-labeled human IFN-γ mRNA 5′-terminal 203 nt transcript (0.08 pmol, 1.25 × 10 5 c.p.m./pmol) was incubated without eIF2 or with 0.3 pmol of eIF2, in the absence or presence of unlabeled HIV-1 T7 transcripts (0.1–1 pmol) (comp RNA) as shown schematically in (C). The autoradiogram shows free and bound RNA (A). Bound RNA is quantitated in ( B ). Relative affinities for eIF2 are summarized in ( C ).
Techniques Used: Labeling, Incubation

Figure Legend Snippet: A 3 nt change in TAR strongly reduces its affinity for eIF2. Uniformly 32 P-labeled 77 nt TAR T7 transcript (0.08 pmol, 1.25 × 10 5 c.p.m./pmol) was incubated without eIF2 or with 0.14 pmol of eIF2, in the absence or presence of 0.1–4 pmol of wild-type (wt) or mutant TAR T7 RNA transcript shown schematically in ( D ) (mutations are denoted in bold). Electrophoresis on a native gel was used to separate free TAR RNA from complexes C1 and C2 ( A ). C2 and C1 are quantitated in ( B ) and ( C ), respectively. In ( E ), activation of PKR by the indicated concentrations of dsRNA, wt or M5 TAR transcript was assayed by phosphorylation of the PKR (68 kDa) and eIF2α (38 kDa) bands. The autoradiogram is shown.
Techniques Used: Labeling, Incubation, Mutagenesis, Electrophoresis, Activation Assay

Figure Legend Snippet: Effect of lower stem mutations in TAR on affinity for eIF2. Uniformly 32 P-labeled human IFN-γ mRNA 5′-terminal 203 nt transcript (0.08 pmol, 1.25 × 10 5 c.p.m./pmol) was incubated without eIF2 or with 0.09 pmol of eIF2, in the absence or presence of 0.01–1 pmol of unlabeled 80 nt wild-type TAR SP6 transcript (TAR) or mutant forms as shown schematically in ( C ). The autoradiogram of a native gel shows free and bound RNA ( A ). Bound RNA is quantitated in ( B ).
Techniques Used: Labeling, Incubation, Mutagenesis
3) Product Images from "The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity"
Article Title: The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity
Journal: Nature immunology
doi: 10.1038/ni.3766

Figure Legend Snippet: mIFIH1 R mediates increased sensitivity to self-RNA ligands (a-e) Control or ADAR- null HEK293T cells were generated by lenti-CRISPR technology. Cell lines were transfected with 1 μg of mIFIH1 risk or non-risk constructs shown in Fig 2 . Cells were analyzed by flow cytometry at 27 hours post-transfection for (a) geometric mean fluorescent intensity (MFI) and (b) percent GFP expression and combined data from four biological replicates are shown. (c-e) Quantitative RT-PCR for IFNB1 mRNA expression in control vs. ADAR- null cells transfected with mIFIH1 NR construct. (c) Representative data from one experiment. (d) Combined data from four biological replicates. (e) Relative levels of IFNB1 mRNA expression in mIFIH1 NR vs. mIFIH1 R transfected ADAR- null cells showing combined data from four biological replicates. Results were normalized by the Livak method as in Fig 2d . Statistical analysis performed using a two-tail student T test. Each data point represents one biological replicate. Error bars represent ± SEM. *p
Techniques Used: Generated, CRISPR, Transfection, Construct, Flow Cytometry, Cytometry, Expressing, Quantitative RT-PCR
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RNA Sequencing Assay:Article Title: Mitigating Antagonism between Transcription and Proliferation Allows Near-Deterministic Cellular Reprogramming Article Snippet: .. In Vitro:Article Title: Pseudouridylation of 7 SK sn RNA promotes 7 SK sn RNP formation to suppress HIV‐1 transcription and escape from latency Article Snippet: .. For 2D‐TLC, Positive Control:Article Title: Contrasting Sex-and Caste-Dependent piRNA Profiles in the Transposon Depleted Haplodiploid Honeybee Apis mellifera Article Snippet: .. Isolation:Article Title: Pseudouridylation of 7 SK sn RNA promotes 7 SK sn RNP formation to suppress HIV‐1 transcription and escape from latency Article Snippet: .. For 2D‐TLC, Labeling:Article Title: Recognition of 5?-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2 Article Snippet: .. Labeled Real-time Polymerase Chain Reaction:Article Title: Activating frataxin expression by repeat-targeted nucleic acids Article Snippet: .. Quantitative PCR Identical volumes of Article Title: The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity Article Snippet: .. To obtain Incubation:Article Title: Recognition of 5?-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2 Article Snippet: .. Labeled Lysis:Article Title: The A946T variant IFIH1 RNA sensor mediates an interferon program that limits viral infection but increases the risk for autoimmunity Article Snippet: .. To obtain Binding Assay:Article Title: Recognition of 5?-terminal TAR structure in human immunodeficiency virus-1 mRNA by eukaryotic translation initiation factor 2 Article Snippet: .. Labeled |