rabbit igg (Alomone Labs)


Structured Review

Rabbit Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Enhanced Hsp70 Expression Protects against Acute Lung Injury by Modulating Apoptotic Pathways"
Article Title: Enhanced Hsp70 Expression Protects against Acute Lung Injury by Modulating Apoptotic Pathways
Journal: PLoS ONE
doi: 10.1371/journal.pone.0026956

Figure Legend Snippet: A. Immunoblotting of whole cytosolic extracts for Caspase 8 and 9. Abbreviations as in . Upper panel : Representative autoradiogram of SDS-PAGE, 30 µg of cytosolic extract/lane. Primary rabbit polyclonal antibody to caspase 8. Secondary goat anti rabbit IgG. .Graphic representation of relative density of cytosolic caspase 8. Middle panel : Representative autoradiogram of SDS-PAGE, 30 µg of cytosolic extracts. Primary rabbit polyclonal antibody to caspase 9, secondary goat anti rabbit IgG. Graph - Graphic representation of relative density (mean +/− standard deviation) of cytosolic caspase 9. * = significantly different from 2CLPPBS and 2CLPAdGFP. Lower panels: Representative autoradiogram of SDS-PAGE, 30 µg of mitochondrial extract/lane. Primary mouse monoclonal antibody to Bcl2, secondary goat anti mouse IgG and primary mouse monoclonal antibody to COX IV, secondary goat anti mouse IgG. COX IV serves as mitochondrial loading control. B. Hsp70 in vivo interaction with apopotosomal Apaf-1. Representative autoradiograms. Samples were immunoprecipitated with a rabbit polyclonal antibody to Apaf-1 and subjected to SDS-PAGE. Upper panels: Immunoblotting with a primary rabbit polyclonal antibody to pro-caspase 9, secondary goat anti rabbit IgG. Middle panels: Immunoblotting with primary mouse monoclonal antibody to Hsp70, secondary goat anti mouse IgG. Lower panels: IgG detection IgG serves as loading control. 250 µg of cytosolic extracts obtained from TO, 2CLPPBS, 2CLPAdHSP or 2CLPAdGFP treated animals sacrificed 48 hrs after the induction of sepsis. C. Hsp70 in vitro, MLE-12 cells, interaction with apopotosomal Apaf-1. Representative autoradiograms. 250 µg of cytosolic extracts obtained from non treated MLE-12 cells (controls), stimulated with tumor necrosis factor (TNF) and treated with AdHSP or AdGFP. Samples were immunoprecipitated with a rabbit polyclonal antibody to Apaf-1 and subjected to SDS-PAGE. Upper panels: Immunoblotting with a primary rabbit polyclonal antibody to pro-caspase 9, secondary goat anti rabbit IgG. Middle panels: Immunoblotting with primary mouse monoclonal antibody to Hsp70, secondary goat anti mouse IgG. Lower panels: IgG serves as loading control. D. Apaf-1 – CARD dissociates from Pro-caspase-9 in the presence of Hsp70. Representative autoradiograms. 100 µg of cytosolic extracts obtained from 2CLPPBS and 2CLAdHSP treated animals, were immunoprecipitated with Pro-caspase-9 and further incubated with GST-Apaf-1 – CARD obtained from BL-21 cells, together with 5 mM ATP and 5 µg/ml human Cytochrome C for 5, 10, 20 and 30 minutes. Samples were subjected to SDS-PAGE, immunoblotted and the membranes were incubated with primary rabbit polyclonal antibody to Cleaved Caspase-9, secondary to goat anti rabbit IgG.
Techniques Used: Western Blot, SDS Page, Standard Deviation, In Vivo, Immunoprecipitation, In Vitro, Incubation

Figure Legend Snippet: A. Representative autoradiograms for pro-caspase 3 and activated (cleaved) Caspase 3. 30 µg of cytosolic (upper panels) and nuclear (lower panel) extracts were subjected to SDS-PAGE. Immunoblotting performed with primary rabbit polyclonal antibody to pro- caspase 3 and secondary goat anti rabbit IgG, primary goat antibody to β-actin and secondary donkey anti goat IgG, primary rabbit polyclonal antibody to active (cleaved) caspase-3 and secondary goat anti rabbit IgG, primary mouse monoclonal antibody to histone (H1) and secondary goat anti mouse IgG.. β-actin and histone serve as loading controls. B. Representative stained fixed tissue section depicting intra-nuclear staining for Caspase 3. Sections obtained from T0 control, 2CLPPBS and 2CLPHSP rats. Tissue isolated 48 hrs after the induction of sepsis. Upper panel : 40× magnifications. Black arrows indicate active caspase 3 stained nuclei. Lower panel : 100× magnification of upper panel. C. Caspase 3 Activity Assay. Graphic representation of relative caspase-3 enzymatic activity (mean +/− standard deviation) * = significantly different from Control and AdHSP+TNF.
Techniques Used: SDS Page, Western Blot, Staining, Isolation, Caspase-3 Activity Assay, Activity Assay, Standard Deviation

Figure Legend Snippet: A and B: Representative Autoradiogram Demonstrating AdHSP treatment disrupts interaction between caspase 8 and caspase 9. Representative autoradiograms. 250 µg of cytosolic extracts were immunoprecipitated with rabbit polyclonal antibody to caspase 8 or caspase-9 and subjected to SDS-PAGE. Immunoblotting performed with either primary rabbit polyclonal antibodies to caspase 9 or caspase-8, secondary goat anti rabbit IgG. Lower panels: IgG detection. IgG serves as loading control. Abbreviations as in . C: Hsp70 disrupts caspases 3, 8 9 and Apaf-1 complexes. 250 µg of cytosolic extracts from lung tissue fractionated via column chromatography, eluted by molecular weight, immunoprecipitated with an antibody to caspase-9 and subjected to 9% SDS-PAGE. Molecular weight of each fraction (kDa) indicated at the top of the figure. Detecting antibodies (anti-caspase 9, anti-caspase 8, anti-pro-caspase 3, anti-Hsp70 and anti-Apaf-1,) noted to the left of the panels. Lower panel: IgG detection. IgG serves as loading control. Abbreviations as in .
Techniques Used: Immunoprecipitation, SDS Page, Western Blot, Column Chromatography, Molecular Weight
anti rabbit igg (Alomone Labs)


Structured Review
Anti Rabbit Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rabbit igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit igg (Alomone Labs)


Structured Review

Rabbit Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system"
Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system
Journal: Fluids and Barriers of the CNS
doi: 10.1186/s12987-020-0176-z

Figure Legend Snippet: List of primary antibodies
Techniques Used: Diagnostic Assay, Derivative Assay
rabbit igg (Alomone Labs)


Structured Review

Rabbit Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system"
Article Title: Aquaporin 1 and the Na + /K + /2Cl − cotransporter 1 are present in the leptomeningeal vasculature of the adult rodent central nervous system
Journal: Fluids and Barriers of the CNS
doi: 10.1186/s12987-020-0176-z

Figure Legend Snippet: List of primary antibodies
Techniques Used: Diagnostic Assay, Derivative Assay
rabbit igg conjugated to horseradish peroxidase (Alomone Labs)


Structured Review
Rabbit Igg Conjugated To Horseradish Peroxidase, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit igg conjugated to horseradish peroxidase/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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rabbit anti sfgfp (Alomone Labs)


Structured Review

Rabbit Anti Sfgfp, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti sfgfp/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A disease mutation reveals a role for Na V 1.9 in acute itch"
Article Title: A disease mutation reveals a role for Na V 1.9 in acute itch
Journal: The Journal of Clinical Investigation
doi: 10.1172/JCI122481

Figure Legend Snippet: (A) After healing, wounds from scratching left marks that resembled bruising (black arrows) in the p.L811P patient. (B) Schematic diagram of the flexible accelerated Neo-STOP tetracycline-inducible (FAST) cassette illustrating the generation of global-knockout NaV1.9 mice (red box) and Cre-mediated expression of sfGFP-tagged NaV1.9 mice (green box). (C) A DRG section from an sfGFP-tagged NaV1.9 (top) and a WT mouse (bottom) showing the overlap between endogenous fluorescent signal (green) and autofluorescence, followed by staining with an antibody against GFP (red). (D) Western blot of DRG tissue from a WT, an sfGFP-NaV1.9, and an NaV1.9–/– mouse stained for GFP. An HSP90 antibody was used as a loading control. (E) Western blot of tissues taken from an sfGFP-NaV1.9 mouse and stained for GFP, stripped, and reprobed for NaV1.9 using a commercial antibody. An HSP90 antibody was used as a loading control. TG, trigeminal ganglia. (F and G) Representative current traces from ND7/23 cell lines expressing WT (black) or sfGFP-NaV1.9 (green) channels. (H–J) Current-voltage (I-V) (H) and deduced conductance-voltage (G-V) (I) and steady-state inactivation (SSI) (J) relationships of WT (black) and sfGFP-NaV1.9 (green). (G-V: WT-NaV1.9 V1/2 = –25.5 ± 0.5 mV, n = 16; GFP-NaV1.9 V1/2 = –24.0 ± 0.2 mV, n = 11, P = 0.52; SSI: WT-NaV1.9 V1/2 = –29.3 ± 3.5 mV, n = 7; GFP-NaV1.9 V1/2 = –26.6 ± 1.7 mV, n = 7, P = 0.49.). Data are represented as mean ± SEM. Scale bar: 50 μm.
Techniques Used: Knock-Out, Expressing, Staining, Western Blot
rabbit anti k v 2 1 (Alomone Labs)


Structured Review
Rabbit Anti K V 2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti k v 2 1/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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secondary antibody anti rabbit fitc (Alomone Labs)


Structured Review
Secondary Antibody Anti Rabbit Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibody anti rabbit fitc/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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secondary antibody anti rabbit fitc (Alomone Labs)


Structured Review
Secondary Antibody Anti Rabbit Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibody anti rabbit fitc/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti rabbit igg (Alomone Labs)


Structured Review
Anti Rabbit Igg, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rabbit igg/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99