btgzi  (New England Biolabs)


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  • 94
    Name:
    BtgZI
    Description:
    BtgZI 500 units
    Catalog Number:
    r0703l
    Price:
    282
    Size:
    500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs btgzi
    BtgZI
    BtgZI 500 units
    https://www.bioz.com/result/btgzi/product/New England Biolabs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    btgzi - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis"

    Article Title: Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-14329-5

    Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .
    Figure Legend Snippet: Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .

    Techniques Used: Sequencing, Plasmid Preparation, Marker

    2) Product Images from "Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis"

    Article Title: Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-14329-5

    Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .
    Figure Legend Snippet: Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .

    Techniques Used: Sequencing, Plasmid Preparation, Marker

    Related Articles

    Clone Assay:

    Article Title: The Exported Chaperone PfHsp70x Is Dispensable for the Plasmodium falciparum Intraerythrocytic Life Cycle
    Article Snippet: .. Briefly, pMK-U6 was digested with BtgZI (New England Biolabs), and annealed oligonucleotides were inserted using In-Fusion HD Cloning kit (Clontech). (iii) pHA-glmS and pHA-M9 were used as donor DNA templates consisting of two homology regions flanking the hemagglutinin (HA) tag and the glmS (or the M9 ) sequences. ..

    Article Title: The Exported Chaperone PfHsp70x Is Dispensable for the Plasmodium falciparum Intraerythrocytic Life Cycle
    Article Snippet: .. Briefly, pL6 was digested with BtgZI (New England Biolabs), and annealed oligonucleotides were inserted using In-Fusion HD cloning kit (Clontech). ..

    other:

    Article Title: Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria
    Article Snippet: Molecular biology enzymes Enzymes used for molecular biology were the following: restriction enzymes BsmBI/Esp3I (Fermentas), BsaI and BtgZI (NEB), T4 DNA ligase (Promega), Phusion DNA Polymerase (Agilent) and KAPA2G (KAPA Biosystems).

    Plasmid Preparation:

    Article Title: Mitochondrial type II NADH dehydrogenase of Plasmodium falciparum (PfNDH2) is dispensable in the asexual blood stages
    Article Snippet: .. The vector, pAIO-Cas9-yDHOD(-), was digested with BtgZI and joined with the annealed oligonucleotide pair by gene assembly (New England Biolabs® , Inc), yielding a pAIO-Cas9-yDHOD(-)-gRNA construct. .. Other gRNA cloning procedures followed our published protocol [ ].

    Article Title: Characterization of MazF-Mediated Sequence-Specific RNA Cleavage in Pseudomonas putida Using Massive Parallel Sequencing
    Article Snippet: .. The pMD19 plasmid encoding the graA gene was digested with Hin dIII (New England Biolabs, Ipswich, MA). pUC19 plasmids encoding synthetic RNA (500–2, 1000–1, 1000–2, 1000–3, and 1000–4) and the pUC19 plasmid encoding 1000–5 synthetic RNA were digested with Bbs I and Btg ZI, respectively (New England Biolabs). .. Digested fragments were purified with a PCR purification kit and then in vitro transcription was carried out with MEGAscript T7 Kit (Life Technologies) according to the manufacturer’s instruction.

    Construct:

    Article Title: Mitochondrial type II NADH dehydrogenase of Plasmodium falciparum (PfNDH2) is dispensable in the asexual blood stages
    Article Snippet: .. The vector, pAIO-Cas9-yDHOD(-), was digested with BtgZI and joined with the annealed oligonucleotide pair by gene assembly (New England Biolabs® , Inc), yielding a pAIO-Cas9-yDHOD(-)-gRNA construct. .. Other gRNA cloning procedures followed our published protocol [ ].

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  • 94
    New England Biolabs btgzi
    Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, <t>BtgZI,</t> BbsI, <t>BsaI,</t> BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .
    Btgzi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btgzi/product/New England Biolabs
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    btgzi - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

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    Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .

    Journal: Scientific Reports

    Article Title: Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis

    doi: 10.1038/s41598-017-14329-5

    Figure Lengend Snippet: Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .

    Article Snippet: BsaI, BbsI, BsmBI and BtgZI were purchased from NEB, AarI from Thermo ScientificTM .

    Techniques: Sequencing, Plasmid Preparation, Marker