mspji  (New England Biolabs)


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    New England Biolabs mspji
    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) <t>MspJI</t> <t>cut</t> <t>DNA</t> oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspji - by Bioz Stars, 2024-05
    93/100 stars

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    1) Product Images from "Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing"

    Article Title: Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae023

    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Figure Legend Snippet: Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).

    Techniques Used: Methylation, Staining

    mspji  (New England Biolabs)


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  • 93

    Structured Review

    New England Biolabs mspji
    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) <t>MspJI</t> <t>cut</t> <t>DNA</t> oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspji - by Bioz Stars, 2024-05
    93/100 stars

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    1) Product Images from "Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing"

    Article Title: Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae023

    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Figure Legend Snippet: Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).

    Techniques Used: Methylation, Staining

    mspji  (New England Biolabs)


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  • 93

    Structured Review

    New England Biolabs mspji
    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) <t>MspJI</t> <t>cut</t> <t>DNA</t> oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspji - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing"

    Article Title: Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae023

    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Figure Legend Snippet: Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).

    Techniques Used: Methylation, Staining

    mspji  (New England Biolabs)


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  • 93

    Structured Review

    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    mspji  (New England Biolabs)


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    New England Biolabs mspji
    A AtSN1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . <t>B</t> <t>Methylation</t> levels of AtSN1 locus determined using Chop-qPCR on digested and undigested DNA. Digestion was performed with <t>MspJI,</t> modification-dependent restriction endonuclease, and data were normalized to AtSN1 level from the undigested DNA and then the reciprocal was calculated to show the relative methylation levels. C AtROS1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . All the results show the average of 3 independent biological replicates and were statistically analyzed against Columbia wild type (Col WT) and significant differences are indicated with asterisks (Anova one-way with Dunnett’s post-hoc test, * < 0.05, ** < 0.01, *** < 0.001 and **** < 0.0001). Error bars represent the mean ± SD, n = 3
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
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    mspji - by Bioz Stars, 2024-05
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    1) Product Images from "Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation"

    Article Title: Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation

    Journal: Plant Cell Reports

    doi: 10.1007/s00299-024-03177-z

    A AtSN1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . B Methylation levels of AtSN1 locus determined using Chop-qPCR on digested and undigested DNA. Digestion was performed with MspJI, modification-dependent restriction endonuclease, and data were normalized to AtSN1 level from the undigested DNA and then the reciprocal was calculated to show the relative methylation levels. C AtROS1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . All the results show the average of 3 independent biological replicates and were statistically analyzed against Columbia wild type (Col WT) and significant differences are indicated with asterisks (Anova one-way with Dunnett’s post-hoc test, * < 0.05, ** < 0.01, *** < 0.001 and **** < 0.0001). Error bars represent the mean ± SD, n = 3
    Figure Legend Snippet: A AtSN1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . B Methylation levels of AtSN1 locus determined using Chop-qPCR on digested and undigested DNA. Digestion was performed with MspJI, modification-dependent restriction endonuclease, and data were normalized to AtSN1 level from the undigested DNA and then the reciprocal was calculated to show the relative methylation levels. C AtROS1 expression levels determined by RT-qPCR analysis of T2 mixed-stage inflorescences. Data were normalized using AtUBC9 and AtACT2 . All the results show the average of 3 independent biological replicates and were statistically analyzed against Columbia wild type (Col WT) and significant differences are indicated with asterisks (Anova one-way with Dunnett’s post-hoc test, * < 0.05, ** < 0.01, *** < 0.001 and **** < 0.0001). Error bars represent the mean ± SD, n = 3

    Techniques Used: Expressing, Quantitative RT-PCR, Methylation, Modification

    mspji  (New England Biolabs)


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    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mspji  (New England Biolabs)


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    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mspji  (New England Biolabs)


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    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mspji  (New England Biolabs)


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    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mspji enzyme  (New England Biolabs)


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    New England Biolabs mspji enzyme
    Mspji Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji enzyme/product/New England Biolabs
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    mspji  (New England Biolabs)


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    New England Biolabs mspji
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mspji
    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) <t>MspJI</t> <t>cut</t> <t>DNA</t> oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Mspji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspji - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs mspji enzyme
    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) <t>MspJI</t> <t>cut</t> <t>DNA</t> oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).
    Mspji Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspji enzyme/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspji enzyme - by Bioz Stars, 2024-05
    93/100 stars
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    Image Search Results


    Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).

    Journal: Nucleic Acids Research

    Article Title: Uncovering the roles of DNA hemi-methylation in transcriptional regulation using MspJI-assisted hemi-methylation sequencing

    doi: 10.1093/nar/gkae023

    Figure Lengend Snippet: Proof of principles and technical statistics of Mhemi-seq. (A) Scheme of strand-specific mCNNR, mCpG, and mCWG detection using Mhemi-seq. (B) MspJI cut DNA oligonucleotides in different manners according to their methylation status. Reaction mix was run on a 20% polyacrylamide TBE gel and visualized by GelRed staining. (C) Motif analysis reveals the specificity of MspJI on CNNR sites in GM12878. (D) Length distribution of inserts in Mhemi-seq library (grey histogram) versus the length distribution simulated using BS-seq results (orange line).

    Article Snippet: A 20 μl reaction was prepared using 10–100 ng of genomic DNA, 10x NEB rCutSmart buffer, 30× MspJI activator, and MspJI (2.5 units per 100 ng of DNA).

    Techniques: Methylation, Staining