btsci enzyme  (New England Biolabs)


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    New England Biolabs btsci enzyme
    Btsci Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    btsci enzyme  (New England Biolabs)


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    New England Biolabs btsci enzyme
    Btsci Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsci enzyme/product/New England Biolabs
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    restriction enzyme btsci  (New England Biolabs)


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    New England Biolabs restriction enzyme btsci
    Restriction Enzyme Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme btsci/product/New England Biolabs
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    btsci  (New England Biolabs)


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    New England Biolabs btsci
    Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme digest assay with btsci  (New England Biolabs)


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    New England Biolabs restriction enzyme digest assay with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Restriction Enzyme Digest Assay With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction enzyme digest assay with btsci - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Emx2 is an essential regulator of ciliated cell development across embryonic tissues"

    Article Title: Emx2 is an essential regulator of ciliated cell development across embryonic tissues

    Journal: bioRxiv

    doi: 10.1101/2024.01.04.574218

    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Figure Legend Snippet: (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Techniques Used: Enzymatic Assay, Staining, Marker, Sequencing

    digestion with btsci  (New England Biolabs)


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    New England Biolabs digestion with btsci
    Digestion With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    digestion with btsci - by Bioz Stars, 2024-05
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    digestion with btsci  (New England Biolabs)


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    New England Biolabs digestion with btsci
    <t>BtsCI</t> digestion of purified aac (6′)- <t>Ib</t> <t>PCR</t> products. Lane M: 100 bp DNA ladder. Lane A: PCR products cut via BtsCI digestion (wild aac(6′)-Ib gene); lane B: a PCR product showing three fragments by BtsCI digestion (wild aac(6′)-Ib + aac(6′)-Ib-cr gene); lane C: undigested PCR product.
    Digestion With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "First Report of aac(6′)-Ib and aac(6′)-Ib-cr Variant Genes Associated with Mutations in gyrA Encoded Fluoroquinolone Resistance in Avian Campylobacter coli Strains Collected in Tunisia"

    Article Title: First Report of aac(6′)-Ib and aac(6′)-Ib-cr Variant Genes Associated with Mutations in gyrA Encoded Fluoroquinolone Resistance in Avian Campylobacter coli Strains Collected in Tunisia

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms242216116

    BtsCI digestion of purified aac (6′)- Ib PCR products. Lane M: 100 bp DNA ladder. Lane A: PCR products cut via BtsCI digestion (wild aac(6′)-Ib gene); lane B: a PCR product showing three fragments by BtsCI digestion (wild aac(6′)-Ib + aac(6′)-Ib-cr gene); lane C: undigested PCR product.
    Figure Legend Snippet: BtsCI digestion of purified aac (6′)- Ib PCR products. Lane M: 100 bp DNA ladder. Lane A: PCR products cut via BtsCI digestion (wild aac(6′)-Ib gene); lane B: a PCR product showing three fragments by BtsCI digestion (wild aac(6′)-Ib + aac(6′)-Ib-cr gene); lane C: undigested PCR product.

    Techniques Used: Purification

    btsci restriction site present  (New England Biolabs)


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    New England Biolabs btsci restriction site present
    Btsci Restriction Site Present, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    digestion with btsci  (New England Biolabs)


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    New England Biolabs digestion with btsci
    Digestion With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    btsci  (New England Biolabs)


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    New England Biolabs btsci
    Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    btsci  (New England Biolabs)


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    New England Biolabs btsci
    Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs btsci enzyme
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    New England Biolabs restriction enzyme digest assay with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Restriction Enzyme Digest Assay With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs digestion with btsci
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Digestion With Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/digestion with btsci/product/New England Biolabs
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    New England Biolabs btsci restriction site present
    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying <t>with</t> <t>BtsCI</t> restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.
    Btsci Restriction Site Present, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Journal: bioRxiv

    Article Title: Emx2 is an essential regulator of ciliated cell development across embryonic tissues

    doi: 10.1101/2024.01.04.574218

    Figure Lengend Snippet: (A) Working model showing emx2 induces prostaglandin biosynthesis for cilia development in zebrafish pronephros in both proximal and distal domain. (B) Image of DNA products after verifying with BtsCI restriction enzyme assay. − denotes samples without BtsCI and + denotes samples with BtsCI. WT embryos and confirmed WT embryos from emx2 el586/el586 line show two bands at 302 bp and 103 bp, while confirmed emx2 −/− embryos show one band at 405 bp. (C) WT embryos stained via WISH using marker for emx2 from 5 ss to 17 ss. Scale bar = 100 μm. (D) 28 hpf whole-mount IF for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI (nucleus, blue) in the distal pronephros of WT and emx2 −/− embryos. Scale bar = 50 μm. (E) Sanger sequencing between WT, WT from emx2 el586/el586 line and emx2 −/− embryos. (F) TALEN target site between WT and emx2 −/− . emx2 −/− has 10-bp deletion compared to WT. Green denotes L and R TALEN target sites. Blue denotes BtsCI restriction site which is disrupted in emx2 −/− embryos.

    Article Snippet: Additionally, restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT, heterozygous or mutant samples.

    Techniques: Enzymatic Assay, Staining, Marker, Sequencing