hpy188iii  (New England Biolabs)


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    Structured Review

    New England Biolabs hpy188iii
    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and <t>Hpy188III.</t> Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Hpy188iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpy188iii/product/New England Biolabs
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    hpy188iii - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

    2) Product Images from "The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains"

    Article Title: The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains

    Journal: eLife

    doi: 10.7554/eLife.60660

    Generation of Hoil-1l C458A/C458A mice. ( A ) Sequences of genomic DNA around C458 codon, gRNAs, and donor oligonucleotide used to target heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1L) C458A mutation. ( B ) Sanger sequencing confirming correct mutations at target sites. ( C ) Genotyping results of Hoil-1l +/+ , Hoil-1l +/C458A , and Hoil-1l C458A/C458A mice. Hpy188III digest of a PCR fragment confirming correct targeting where a silent mutation is inserted. ( D ) Immunoblot analysis of linear ubiquitin chain assembly complex (LUBAC) component expression in mouse embryonic fibroblasts (MEFs) derived from Hoil-1l +/+ and Hoil-1l C458A/C458A mice.
    Figure Legend Snippet: Generation of Hoil-1l C458A/C458A mice. ( A ) Sequences of genomic DNA around C458 codon, gRNAs, and donor oligonucleotide used to target heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1L) C458A mutation. ( B ) Sanger sequencing confirming correct mutations at target sites. ( C ) Genotyping results of Hoil-1l +/+ , Hoil-1l +/C458A , and Hoil-1l C458A/C458A mice. Hpy188III digest of a PCR fragment confirming correct targeting where a silent mutation is inserted. ( D ) Immunoblot analysis of linear ubiquitin chain assembly complex (LUBAC) component expression in mouse embryonic fibroblasts (MEFs) derived from Hoil-1l +/+ and Hoil-1l C458A/C458A mice.

    Techniques Used: Mouse Assay, Mutagenesis, Sequencing, Polymerase Chain Reaction, Expressing, Derivative Assay

    3) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

    4) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

    5) Product Images from "Association of Genetic Variants of KCNJ11 and KCNQ1 Genes with Risk of Type 2 Diabetes Mellitus (T2DM) in the Indian Population: A Case-Control Study"

    Article Title: Association of Genetic Variants of KCNJ11 and KCNQ1 Genes with Risk of Type 2 Diabetes Mellitus (T2DM) in the Indian Population: A Case-Control Study

    Journal: International Journal of Endocrinology

    doi: 10.1155/2020/5924756

    3% agarose gel electrophoresis for the digested PCR product with Hpy188III (NEB) and Ava I (NEB), respectively. (a) KCNJ11, L-100 bp DNA ladder; W- Homo Wild (A/A); M- Homo Mutant (G/G); H- Hetero (A/G) and (b) KCNQ1, L-100 bp DNA ladder; W- Homo Wild (A/A); M- Homo Mutant (C/C); H- Hetero (A/C).
    Figure Legend Snippet: 3% agarose gel electrophoresis for the digested PCR product with Hpy188III (NEB) and Ava I (NEB), respectively. (a) KCNJ11, L-100 bp DNA ladder; W- Homo Wild (A/A); M- Homo Mutant (G/G); H- Hetero (A/G) and (b) KCNQ1, L-100 bp DNA ladder; W- Homo Wild (A/A); M- Homo Mutant (C/C); H- Hetero (A/C).

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Antiviral Assay, Mutagenesis

    6) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

    7) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

    8) Product Images from "FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease"

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    Journal: Journal of medical genetics

    doi: 10.1136/jmg.2009.076703

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).
    Figure Legend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Techniques Used: Sequencing

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    New England Biolabs hpy 188iii restriction enzyme
    COBRA assay of ERα gene promoter C in wild-type and LTED cells. First PCR products of bisulfite converted DNA from wild-type or LTED cells using primers ERPROCBSF and ERPROCBSR were digested with restriction enzyme <t>Hpy</t> <t>188III</t> as described in Section 2. Rectangle indicates the position of the CpG site to be tested. Underline indicates the recognition site of Hpy 188III that will be generated by bisulfite conversion of methylated DNA, but not unmethylated one. An image of the polyacrylamide gel electrophoresis is shown below. U, untreated. D, digested with Hpy 188III.
    Hpy 188iii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpy 188iii restriction enzyme/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpy 188iii restriction enzyme - by Bioz Stars, 2022-08
    93/100 stars
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    COBRA assay of ERα gene promoter C in wild-type and LTED cells. First PCR products of bisulfite converted DNA from wild-type or LTED cells using primers ERPROCBSF and ERPROCBSR were digested with restriction enzyme Hpy 188III as described in Section 2. Rectangle indicates the position of the CpG site to be tested. Underline indicates the recognition site of Hpy 188III that will be generated by bisulfite conversion of methylated DNA, but not unmethylated one. An image of the polyacrylamide gel electrophoresis is shown below. U, untreated. D, digested with Hpy 188III.

    Journal: The Journal of steroid biochemistry and molecular biology

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells

    doi: 10.1016/j.jsbmb.2006.12.104

    Figure Lengend Snippet: COBRA assay of ERα gene promoter C in wild-type and LTED cells. First PCR products of bisulfite converted DNA from wild-type or LTED cells using primers ERPROCBSF and ERPROCBSR were digested with restriction enzyme Hpy 188III as described in Section 2. Rectangle indicates the position of the CpG site to be tested. Underline indicates the recognition site of Hpy 188III that will be generated by bisulfite conversion of methylated DNA, but not unmethylated one. An image of the polyacrylamide gel electrophoresis is shown below. U, untreated. D, digested with Hpy 188III.

    Article Snippet: For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel.

    Techniques: Combined Bisulfite Restriction Analysis Assay, Polymerase Chain Reaction, Generated, Methylation, Polyacrylamide Gel Electrophoresis

    Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Journal: Journal of medical genetics

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease

    doi: 10.1136/jmg.2009.076703

    Figure Lengend Snippet: Testing for the 4qA161 haplotype by differential digestion with Hpy188I and Hpy188III. Genomic DNAs and human-rodent somatic cell hybrids (GM14193, GM11687, and GM10926 containing chr4, chr4, and chr10, respectively; Coriell Institute) were analysed for overlapping Hpy188I (purple) or Hpy188III (blue) sites. Whether one or two 4qA161 alleles were present was subsequently determined by the sequencing assay. GM11448 and GM10115 containing chr4 were negative for 4qA161 (not shown) (A). The Hpy188I or Hpy188III sites are indicated for the forward strand in the SNP-rich region of 4qA161, the other main 4qA alleles, and the predominant 10qA allele (B).

    Article Snippet: To analyse the association of the 4qA161 haplotype with FSHD, we identified a SNP ( , SNP3) located 307 bp proximal to D4Z4 (GenBank , nt 4519) at overlapping recognition sites for Hpy188I and Hpy188III ( ).

    Techniques: Sequencing

    Generation of Hoil-1l C458A/C458A mice. ( A ) Sequences of genomic DNA around C458 codon, gRNAs, and donor oligonucleotide used to target heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1L) C458A mutation. ( B ) Sanger sequencing confirming correct mutations at target sites. ( C ) Genotyping results of Hoil-1l +/+ , Hoil-1l +/C458A , and Hoil-1l C458A/C458A mice. Hpy188III digest of a PCR fragment confirming correct targeting where a silent mutation is inserted. ( D ) Immunoblot analysis of linear ubiquitin chain assembly complex (LUBAC) component expression in mouse embryonic fibroblasts (MEFs) derived from Hoil-1l +/+ and Hoil-1l C458A/C458A mice.

    Journal: eLife

    Article Title: The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains

    doi: 10.7554/eLife.60660

    Figure Lengend Snippet: Generation of Hoil-1l C458A/C458A mice. ( A ) Sequences of genomic DNA around C458 codon, gRNAs, and donor oligonucleotide used to target heme-oxidized IRP2 ubiquitin ligase 1 (HOIL-1L) C458A mutation. ( B ) Sanger sequencing confirming correct mutations at target sites. ( C ) Genotyping results of Hoil-1l +/+ , Hoil-1l +/C458A , and Hoil-1l C458A/C458A mice. Hpy188III digest of a PCR fragment confirming correct targeting where a silent mutation is inserted. ( D ) Immunoblot analysis of linear ubiquitin chain assembly complex (LUBAC) component expression in mouse embryonic fibroblasts (MEFs) derived from Hoil-1l +/+ and Hoil-1l C458A/C458A mice.

    Article Snippet: Routine genotyping of mice was done by digestion with Hpy188III (NEB R0622) and analysis on a 2% agarose gel.

    Techniques: Mouse Assay, Mutagenesis, Sequencing, Polymerase Chain Reaction, Expressing, Derivative Assay