hpya v digestion  (New England Biolabs)


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  • 90
    Name:
    HpyAV
    Description:
    HpyAV 500 units
    Catalog Number:
    R0621L
    Price:
    285
    Size:
    500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs hpya v digestion
    HpyAV
    HpyAV 500 units
    https://www.bioz.com/result/hpya v digestion/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpya v digestion - by Bioz Stars, 2019-06
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621). .. The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621).

    Mutagenesis:

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: The pwr-2 mutation was genotyped using primers EN1_XcmIF and 3G52250R1, which fail to amplify a fragment in the homozygous mutant. .. The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621). .. For miR172d-1 , the primers R194geno-F and R194geno-R were used to amplify the genomic fragment.

    Mouse Assay:

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: The conditions of PCR reaction were as described in the section Genotyping of F2 mice. .. Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n).

    Real-time Polymerase Chain Reaction:

    Article Title: Fold or hold: experimental evolution in vitro
    Article Snippet: Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks. .. Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks.

    Polymerase Chain Reaction:

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: The conditions of PCR reaction were as described in the section Genotyping of F2 mice. .. Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n). .. The products were electrophoresed in 3% agarose gel containing 80% of MetaPhor® Agarose (Cambrex Bio Science Rockland, Inc., Rockland, ME) and 20% of UltraPure™ Agarose (Invitrogen, Carlsbad, CA) for 15 min to 2 h at 150 V.

    other:

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621).

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n).

    Article Title: Fold or hold: experimental evolution in vitro
    Article Snippet: Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks.

    Sequencing:

    Article Title: Fold or hold: experimental evolution in vitro
    Article Snippet: Upper-case letters indicate positions where Oli has the same sequence as the original hairpin, and lower-case letters indicate positions where the Oli sequence differs from the original hairpin. .. Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks.

    Binding Assay:

    Article Title: Fold or hold: experimental evolution in vitro
    Article Snippet: To form a structure capable of self-priming, Oli molecules must pair the 3′ end indicated in bold with the complementary tract near the 5′ end indicated in bold. .. Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks. .. The recognition sequences of BsaI and HpyAV are GGTCTCN and CCTTC(N6 ), respectively.

    Derivative Assay:

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: To map precisely Tbbr2 on STS derived segment of strain CcS-11 on proximal part of chromosome 12 we used 8 microsatellite markers: D12Mit10a, D12Mit11, D12Mit209, D12Mit182, D12Mit104, D12Mit240, D12Mit170, Dtnb (dystrobrevin, beta) and 4 SNPs; rs48212577, rs4229232, rs50154157 and rs50776991 (Generi Biotech, Hradec Králové, Czech Republic). .. Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n).

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  • 90
    New England Biolabs hpya v digestion
    Hpya V Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpya v digestion/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpya v digestion - by Bioz Stars, 2019-06
    90/100 stars
      Buy from Supplier

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