Article Title: Mutations in Rv2983 as a novel determinant of resistance to nitroimidazole drugs in Mycobacterium tuberculosis
Figure Lengend Snippet: Complementation of B101 mutant with Rv2983 . A. Schematic diagram of genomic DNA of M. tuberculosis strains after digestion with restriction enzyme Acc65I; B. Result of southern blot confirmed expected DNA fragments after Acc65I digestion using DIG-labeled Rv2983 probe (H37Rv: 6.3 kb; Rv2983 mutant: 6.3 kb; complemented strains: 6.3 and 3.5 kb).
Article Snippet: Acc65I-digested (NE biolabs) genomic DNA of the wild type, the B101 mutant and the B101pRv2983 and B101phsp60 -Rv2983 complemented strains was separated on agarose gel and transferred onto positively-charged nylon-membrane (GE).
Techniques: Mutagenesis, Southern Blot, Labeling