radicicol  (Alomone Labs)


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    Name:
    Radicicol
    Description:
    Radicicol is an antifungal antibiotic originally discovered to be a tyrosine kinase inhibitor The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol In addition it also exhibits anti angiogenic activity xenopus oocytes and reduces the expression of VEGF
    Catalog Number:
    R-370
    Price:
    554.0
    Category:
    Small Molecule
    Source:
    Humicola fuscoatra.
    Applications:
    0
    Purity:
    >99%
    Size:
    1 Vials containing 1 mg each
    Format:
    Lyophilized/solid.
    Formula:
    C18H17ClO6
    Molecular Weight:
    364.78
    Molecule Name:
    (1aR,2Z,4E,14R,15aR)-8-Chloro-1a,14,15,15atetrahydro-9 ,11-dihydroxy-14-methyl-6H-oxireno[e][2]benzoxacyclotetradecin-6,12(7H)-dione.
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    Structured Review

    Alomone Labs radicicol
    Radicicol
    Radicicol is an antifungal antibiotic originally discovered to be a tyrosine kinase inhibitor The specific inhibition of the chaperone Hsp90 confers anticancer properties to Radicicol In addition it also exhibits anti angiogenic activity xenopus oocytes and reduces the expression of VEGF
    https://www.bioz.com/result/radicicol/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    radicicol - by Bioz Stars, 2021-09
    90/100 stars

    Images

    1) Product Images from "Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus"

    Article Title: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus

    Journal: Epigenetics & Chromatin

    doi: 10.1186/s13072-017-0166-9

    HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3
    Figure Legend Snippet: HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3

    Techniques Used: Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Transfection, Expressing, Western Blot

    2) Product Images from "Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression"

    Article Title: Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0809669106

    Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx
    Figure Legend Snippet: Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Techniques Used: Inhibition

    Related Articles

    other:

    Article Title: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus
    Article Snippet: Chemicals and antibodiesThe following chemicals were used in this study: flavopiridol (F3055, Sigma-Aldrich), radicicol (R-370, Alomone), sodium salicylate (S3007, Sigma-Aldrich), copper sulphate (102790, Merck).

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    Alomone Labs radicicol
    HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), <t>radicicol,</t> FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3
    Radicicol, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/radicicol/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    radicicol - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

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    HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3

    Journal: Epigenetics & Chromatin

    Article Title: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus

    doi: 10.1186/s13072-017-0166-9

    Figure Lengend Snippet: HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3

    Article Snippet: Chemicals and antibodiesThe following chemicals were used in this study: flavopiridol (F3055, Sigma-Aldrich), radicicol (R-370, Alomone), sodium salicylate (S3007, Sigma-Aldrich), copper sulphate (102790, Merck).

    Techniques: Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Transfection, Expressing, Western Blot

    Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression

    doi: 10.1073/pnas.0809669106

    Figure Lengend Snippet: Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Article Snippet: A total of 3 × 106 /ml cells per well in a 6-well plate were seeded 1 day before drug treatment and treated with different concentrations (3, 5, 10, 20, 40, and 80 μM) of radicicol dissolved in DMSO (Alomone labs) ( A and data not shown) for 4 h. The Kc cells treated with equivalent amounts of DMSO vehicle were used as mock-treated cells.

    Techniques: Inhibition