rnase a  (Gold Biotechnology Inc)


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    Structured Review

    Gold Biotechnology Inc rnase a
    Rnase A, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a/product/Gold Biotechnology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2022-09
    91/100 stars

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    Gold Biotechnology Inc abscisic acid
    Protein and DNA-based recombinase logic gates. ( a ) A two-input protein-based AND gate is created by splitting Flp recombinase at two locations (S 1 = 27, S 2 = 396) and fusing to the gibberellin (GIB) and <t>abscisic</t> acid (ABA)-associated chemical inducible dimerization domains. ( b ) Plotted results indicate GFP mean fluorescence intensity (M.F.I.) of four conditions of GIB and ABA. ( c ) Four chemical inducible recombinases can be used together to generate a DNA-based 4-input logic gate; these include a gibberellin-inducible split Bxb1 (S = 468), 4-hydroxytamoxifen (4OHT)-inducible ER T2 -Cre-ER T2 , abscisic acid (ABA)-inducible split Flp (S = 396), and rapalog (RAP)-inducible split PhiC31 (S = 571). Drugs were added two hours post-transfection. ( d ) Plotted results indicate GFP M.F.I. of sixteen conditions of GIB, 4OHT, ABA and RAP. Error bars of M.F.I. indicate the arithmetic standard error of the mean between three transfected cell cultures.
    Abscisic Acid, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abscisic acid/product/Gold Biotechnology Inc
    Average 93 stars, based on 1 article reviews
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    abscisic acid - by Bioz Stars, 2022-09
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    91
    Gold Biotechnology Inc rnase a
    Protein and DNA-based recombinase logic gates. ( a ) A two-input protein-based AND gate is created by splitting Flp recombinase at two locations (S 1 = 27, S 2 = 396) and fusing to the gibberellin (GIB) and <t>abscisic</t> acid (ABA)-associated chemical inducible dimerization domains. ( b ) Plotted results indicate GFP mean fluorescence intensity (M.F.I.) of four conditions of GIB and ABA. ( c ) Four chemical inducible recombinases can be used together to generate a DNA-based 4-input logic gate; these include a gibberellin-inducible split Bxb1 (S = 468), 4-hydroxytamoxifen (4OHT)-inducible ER T2 -Cre-ER T2 , abscisic acid (ABA)-inducible split Flp (S = 396), and rapalog (RAP)-inducible split PhiC31 (S = 571). Drugs were added two hours post-transfection. ( d ) Plotted results indicate GFP M.F.I. of sixteen conditions of GIB, 4OHT, ABA and RAP. Error bars of M.F.I. indicate the arithmetic standard error of the mean between three transfected cell cultures.
    Rnase A, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a/product/Gold Biotechnology Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2022-09
    91/100 stars
      Buy from Supplier

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    Protein and DNA-based recombinase logic gates. ( a ) A two-input protein-based AND gate is created by splitting Flp recombinase at two locations (S 1 = 27, S 2 = 396) and fusing to the gibberellin (GIB) and abscisic acid (ABA)-associated chemical inducible dimerization domains. ( b ) Plotted results indicate GFP mean fluorescence intensity (M.F.I.) of four conditions of GIB and ABA. ( c ) Four chemical inducible recombinases can be used together to generate a DNA-based 4-input logic gate; these include a gibberellin-inducible split Bxb1 (S = 468), 4-hydroxytamoxifen (4OHT)-inducible ER T2 -Cre-ER T2 , abscisic acid (ABA)-inducible split Flp (S = 396), and rapalog (RAP)-inducible split PhiC31 (S = 571). Drugs were added two hours post-transfection. ( d ) Plotted results indicate GFP M.F.I. of sixteen conditions of GIB, 4OHT, ABA and RAP. Error bars of M.F.I. indicate the arithmetic standard error of the mean between three transfected cell cultures.

    Journal: bioRxiv

    Article Title: High-performance chemical and light-inducible recombinases in mammalian cells and mice

    doi: 10.1101/747121

    Figure Lengend Snippet: Protein and DNA-based recombinase logic gates. ( a ) A two-input protein-based AND gate is created by splitting Flp recombinase at two locations (S 1 = 27, S 2 = 396) and fusing to the gibberellin (GIB) and abscisic acid (ABA)-associated chemical inducible dimerization domains. ( b ) Plotted results indicate GFP mean fluorescence intensity (M.F.I.) of four conditions of GIB and ABA. ( c ) Four chemical inducible recombinases can be used together to generate a DNA-based 4-input logic gate; these include a gibberellin-inducible split Bxb1 (S = 468), 4-hydroxytamoxifen (4OHT)-inducible ER T2 -Cre-ER T2 , abscisic acid (ABA)-inducible split Flp (S = 396), and rapalog (RAP)-inducible split PhiC31 (S = 571). Drugs were added two hours post-transfection. ( d ) Plotted results indicate GFP M.F.I. of sixteen conditions of GIB, 4OHT, ABA and RAP. Error bars of M.F.I. indicate the arithmetic standard error of the mean between three transfected cell cultures.

    Article Snippet: Small-molecule chemical induction1000X stocks of abscisic acid (100mM, Gold Biotechnology Cat. No. A-050-500) and gibberellin ester (10mM, Toronto Research Chemicals Cat. No. G377500) were made in 100% ethanol and stored at −20°C.

    Techniques: Fluorescence, Transfection

    Swapping chemical-inducible dimerization domains yield a large repertoire of small-molecule inducible recombinases. ( a ) A Signal-to-Noise (SNR) metric can be used to capture distinguishability of “on” and “off” states, accounting for both the absolute difference in mean signal expression and spread (“noise”) of the distributions. SNR dynamics in units of decibels (dB) were captured over 100 hours ( b ) post-transfection for particular amino acid splits of inducible Cre, Flp and PhiC31 systems incorporating the gibberellin (GIB), rapalog (RAP) and abscisic acid (ABA) dimerization domains GAI/GID1, FKBP/FRB and PYL/ABI, respectively. A 4-hydroxytamoxifen (4OHT)-inducible pCAG-ER T2 -Cre-ER T2 construct is also included in ( b ). Various splits of Cre ( c and d ) Flp ( e and f ), VCre ( g and h ), PhiC31 ( i and j ) TP901 ( k and l ) and Bxb1 ( m and n ) were incorporated with the GIB-, RAP- and ABA-associated dimerization domains. Measurements of GFP mean fluorescence intensity (M.F.I.) in units of molecules of equivalent fluorescein (MEFL) and SNR were made for plus drug (colored blue, purple, and pink squares corresponding to gibberellin, rapalog, and abscisic acid added two hours post-transfection) and minus drug (white squares) conditions. Constitutive recombinases (black squares) and blank vectors (gray squares) were also transfected as a comparison. Red diamonds indicate SNR score. Error bars of M.F.I. indicate the geometric standard deviation of means for three transfected cell cultures.

    Journal: bioRxiv

    Article Title: High-performance chemical and light-inducible recombinases in mammalian cells and mice

    doi: 10.1101/747121

    Figure Lengend Snippet: Swapping chemical-inducible dimerization domains yield a large repertoire of small-molecule inducible recombinases. ( a ) A Signal-to-Noise (SNR) metric can be used to capture distinguishability of “on” and “off” states, accounting for both the absolute difference in mean signal expression and spread (“noise”) of the distributions. SNR dynamics in units of decibels (dB) were captured over 100 hours ( b ) post-transfection for particular amino acid splits of inducible Cre, Flp and PhiC31 systems incorporating the gibberellin (GIB), rapalog (RAP) and abscisic acid (ABA) dimerization domains GAI/GID1, FKBP/FRB and PYL/ABI, respectively. A 4-hydroxytamoxifen (4OHT)-inducible pCAG-ER T2 -Cre-ER T2 construct is also included in ( b ). Various splits of Cre ( c and d ) Flp ( e and f ), VCre ( g and h ), PhiC31 ( i and j ) TP901 ( k and l ) and Bxb1 ( m and n ) were incorporated with the GIB-, RAP- and ABA-associated dimerization domains. Measurements of GFP mean fluorescence intensity (M.F.I.) in units of molecules of equivalent fluorescein (MEFL) and SNR were made for plus drug (colored blue, purple, and pink squares corresponding to gibberellin, rapalog, and abscisic acid added two hours post-transfection) and minus drug (white squares) conditions. Constitutive recombinases (black squares) and blank vectors (gray squares) were also transfected as a comparison. Red diamonds indicate SNR score. Error bars of M.F.I. indicate the geometric standard deviation of means for three transfected cell cultures.

    Article Snippet: Small-molecule chemical induction1000X stocks of abscisic acid (100mM, Gold Biotechnology Cat. No. A-050-500) and gibberellin ester (10mM, Toronto Research Chemicals Cat. No. G377500) were made in 100% ethanol and stored at −20°C.

    Techniques: Expressing, Transfection, Construct, Fluorescence, Standard Deviation