giiib  (Alomone Labs)


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    Alomone Labs giiib
    Giiib, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    giiib - by Bioz Stars, 2022-07
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    Alomone Labs n 270 human neurotrophin 4
    NT-3 and <t>NT-4</t> do not impact gephyrin clustering. A–A’’ , eGFP-gephyrin transfected neurons treated with NT-3 or <t>NT-4</t> (90 min). B , quantification shows BDNF-specific effect on eGFP-gephyrin cluster size reduction. C , quantification of eGFP-gephyrin cluster density after BDNF, NT-3, or NT-4 treatments. D–D’’’ , morphology of denritic segments transfected with eGFP-gephyrin and staining for vGAT presynaptic terminals. E , quantification of eGFP-gephyrin cluster size after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. F , quantification of eGFP-gephyrin cluster density after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. The data were quantified from four independent experiments and 15 neurons/condition. Two-way ANOVA, Bonferroni post hoc comparison. Error bars st.dev. Scale bar 10 μm. ∗∗∗ p
    N 270 Human Neurotrophin 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n 270 human neurotrophin 4/product/Alomone Labs
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    94
    Alomone Labs human recombinant neurotrophins
    NO dependent modulation of synaptic release of BDNF and NT-3 . Hippocampal neurons were transfected with BDNF-GFP (BDNF) or NT-3-GFP (NT-3) and monitored for neurotrophin secretion. (A,C) Averaged depolarization-induced (50 mM K + ) release of NTs vs. negative control. ( B,D) Mean residual fluorescence 300s after stimulation. (A,B) Preincubation with the NO donor SNP (100 μM, 5 min) reduced depolarization-induced secretion of <t>neurotrophins.</t> (C,D) Preincubation and subsequent superfusion with the NOS inhibitor L–NMMA (300 μM, 5 min) during depolarization did not change the amount of neurotrophins that was released. Experiments were performed in the presence of 10 μM DNQX, 200 μM D,L-APV, and 10 μM gabazine, to avoid secondary effects via transmitter secretion. Negative control cells were superfused with HBS throughout. * p vs. control
    Human Recombinant Neurotrophins, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant neurotrophins/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant neurotrophins - by Bioz Stars, 2022-07
    94/100 stars
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    NT-3 and NT-4 do not impact gephyrin clustering. A–A’’ , eGFP-gephyrin transfected neurons treated with NT-3 or NT-4 (90 min). B , quantification shows BDNF-specific effect on eGFP-gephyrin cluster size reduction. C , quantification of eGFP-gephyrin cluster density after BDNF, NT-3, or NT-4 treatments. D–D’’’ , morphology of denritic segments transfected with eGFP-gephyrin and staining for vGAT presynaptic terminals. E , quantification of eGFP-gephyrin cluster size after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. F , quantification of eGFP-gephyrin cluster density after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. The data were quantified from four independent experiments and 15 neurons/condition. Two-way ANOVA, Bonferroni post hoc comparison. Error bars st.dev. Scale bar 10 μm. ∗∗∗ p

    Journal: The Journal of Biological Chemistry

    Article Title: Trophic factor BDNF inhibits GABAergic signaling by facilitating dendritic enrichment of SUMO E3 ligase PIAS3 and altering gephyrin scaffold

    doi: 10.1016/j.jbc.2022.101840

    Figure Lengend Snippet: NT-3 and NT-4 do not impact gephyrin clustering. A–A’’ , eGFP-gephyrin transfected neurons treated with NT-3 or NT-4 (90 min). B , quantification shows BDNF-specific effect on eGFP-gephyrin cluster size reduction. C , quantification of eGFP-gephyrin cluster density after BDNF, NT-3, or NT-4 treatments. D–D’’’ , morphology of denritic segments transfected with eGFP-gephyrin and staining for vGAT presynaptic terminals. E , quantification of eGFP-gephyrin cluster size after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. F , quantification of eGFP-gephyrin cluster density after treating neurons with TrkB-Fc and BDNF, NT-3, or NT-4. The data were quantified from four independent experiments and 15 neurons/condition. Two-way ANOVA, Bonferroni post hoc comparison. Error bars st.dev. Scale bar 10 μm. ∗∗∗ p

    Article Snippet: Transfected cells were treated 90 min with hBDNF (10 ng/ml, Alomone Labs #B-250), NT-3 (10 ng/ml, Alomone Labs #N-260), or NT-4 (10 ng/ml, Alomone Labs #N-270) and/or rh TrKB-Fc (1 μg/ml, R & D Systems #688-TK-100).

    Techniques: Transfection, Staining

    NO dependent modulation of synaptic release of BDNF and NT-3 . Hippocampal neurons were transfected with BDNF-GFP (BDNF) or NT-3-GFP (NT-3) and monitored for neurotrophin secretion. (A,C) Averaged depolarization-induced (50 mM K + ) release of NTs vs. negative control. ( B,D) Mean residual fluorescence 300s after stimulation. (A,B) Preincubation with the NO donor SNP (100 μM, 5 min) reduced depolarization-induced secretion of neurotrophins. (C,D) Preincubation and subsequent superfusion with the NOS inhibitor L–NMMA (300 μM, 5 min) during depolarization did not change the amount of neurotrophins that was released. Experiments were performed in the presence of 10 μM DNQX, 200 μM D,L-APV, and 10 μM gabazine, to avoid secondary effects via transmitter secretion. Negative control cells were superfused with HBS throughout. * p vs. control

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BDNF-induced nitric oxide signals in cultured rat hippocampal neurons: time course, mechanism of generation, and effect on neurotrophin secretion

    doi: 10.3389/fncel.2014.00323

    Figure Lengend Snippet: NO dependent modulation of synaptic release of BDNF and NT-3 . Hippocampal neurons were transfected with BDNF-GFP (BDNF) or NT-3-GFP (NT-3) and monitored for neurotrophin secretion. (A,C) Averaged depolarization-induced (50 mM K + ) release of NTs vs. negative control. ( B,D) Mean residual fluorescence 300s after stimulation. (A,B) Preincubation with the NO donor SNP (100 μM, 5 min) reduced depolarization-induced secretion of neurotrophins. (C,D) Preincubation and subsequent superfusion with the NOS inhibitor L–NMMA (300 μM, 5 min) during depolarization did not change the amount of neurotrophins that was released. Experiments were performed in the presence of 10 μM DNQX, 200 μM D,L-APV, and 10 μM gabazine, to avoid secondary effects via transmitter secretion. Negative control cells were superfused with HBS throughout. * p vs. control

    Article Snippet: Reagents K252a and human recombinant neurotrophins were purchased from Alamone Labs (Jerusalem, Israel).

    Techniques: Transfection, Negative Control, Fluorescence

    Measurement of synaptic release of neurotrophins . (A) Hippocampal neurons were co-transfected at 8-DIV with BDNF-GFP and PSD95-DsRed. Co-localization of both proteins was monitored at 10–11 DIV. Boxed areas on the left are shown at higher magnification on the right. Postsynaptic vesicle clusters of BDNF-GFP (green) were identified by co-localization with PSD95-DsRed (Red). (B) Time course of postsynaptic BDNF-GFP and NT-3-GFP release in response to depolarization with elevated K + (50 mM, 300s). (C) Residual fluorescence of postsynaptic BDNF-GFP and NT-3-GFP after 300 s depolarization. Experiments were performed in the presence of 10 μM DNQX, 200 μM D,L-APV, and 10 μM gabazine, to avoid secondary effects via transmitter secretion. ** p vs control

    Journal: Frontiers in Cellular Neuroscience

    Article Title: BDNF-induced nitric oxide signals in cultured rat hippocampal neurons: time course, mechanism of generation, and effect on neurotrophin secretion

    doi: 10.3389/fncel.2014.00323

    Figure Lengend Snippet: Measurement of synaptic release of neurotrophins . (A) Hippocampal neurons were co-transfected at 8-DIV with BDNF-GFP and PSD95-DsRed. Co-localization of both proteins was monitored at 10–11 DIV. Boxed areas on the left are shown at higher magnification on the right. Postsynaptic vesicle clusters of BDNF-GFP (green) were identified by co-localization with PSD95-DsRed (Red). (B) Time course of postsynaptic BDNF-GFP and NT-3-GFP release in response to depolarization with elevated K + (50 mM, 300s). (C) Residual fluorescence of postsynaptic BDNF-GFP and NT-3-GFP after 300 s depolarization. Experiments were performed in the presence of 10 μM DNQX, 200 μM D,L-APV, and 10 μM gabazine, to avoid secondary effects via transmitter secretion. ** p vs control

    Article Snippet: Reagents K252a and human recombinant neurotrophins were purchased from Alamone Labs (Jerusalem, Israel).

    Techniques: Transfection, Fluorescence

    Immunolocalization of BDNF, NT-4, trkB, and p75 NTR at the neuromuscular synapses. A–D , Triple labeling of the neurotrophins BDNF ( A ) and NT-4 ( B ) and their receptors trkB ( C ) and p75 NTR ( D ) (green fluorescence) with syntaxin (blue fluorescence) and AChR-α bungarotoxin (red fluorescence). We used plastic-embedded semithin (0.5 to 0.7 μm) cross-sections (STS) as a tool for high-resolution, triple-labeling immunofluorescence analysis. E , Example of triple labeling of syntaxin (green fluorescence), S-100 (blue fluorescence), and nAChR-α bungarotoxin (red fluorescence), which proves that the molecular markers of the three synaptic cells are separated well. F , High-resolution images of synaptic boutons with the terminal axon labeled for one of the proteins in green in the nerve terminal area between the blue Schwann cell and the red nAChR cluster. Scale bars: A–E , 10 μm; F , 1 μm.

    Journal: The Journal of Neuroscience

    Article Title: The Interaction between Tropomyosin-Related Kinase B Receptors and Presynaptic Muscarinic Receptors Modulates Transmitter Release in Adult Rodent Motor Nerve Terminals

    doi: 10.1523/JNEUROSCI.2676-10.2010

    Figure Lengend Snippet: Immunolocalization of BDNF, NT-4, trkB, and p75 NTR at the neuromuscular synapses. A–D , Triple labeling of the neurotrophins BDNF ( A ) and NT-4 ( B ) and their receptors trkB ( C ) and p75 NTR ( D ) (green fluorescence) with syntaxin (blue fluorescence) and AChR-α bungarotoxin (red fluorescence). We used plastic-embedded semithin (0.5 to 0.7 μm) cross-sections (STS) as a tool for high-resolution, triple-labeling immunofluorescence analysis. E , Example of triple labeling of syntaxin (green fluorescence), S-100 (blue fluorescence), and nAChR-α bungarotoxin (red fluorescence), which proves that the molecular markers of the three synaptic cells are separated well. F , High-resolution images of synaptic boutons with the terminal axon labeled for one of the proteins in green in the nerve terminal area between the blue Schwann cell and the red nAChR cluster. Scale bars: A–E , 10 μm; F , 1 μm.

    Article Snippet: Stock solutions used were as follows: anti-BDNF (G2610; Promega), 50 μg/ml; anti-NT-4 (AB1781; Millipore Bioscience Research Reagents), 50 μg/ml; anti-trkB (clone 47/trkB; 610102; BD Transduction Laboratories), 250 μg/ml; human BDNF (Alomone Labs), 0.37 μ m ; human NT-4 (Alomone Labs), 40 n m ; K-252a (Calbiochem), 5 m m in DMSO; K-252b (Calbiochem), 5 m m ; Pep5 (Calbiochem), 100 μ m ; recombinant human trkB/Fc Chimera (trkB-Fc; 688-TK; R & D Systems), 100 μg/ml.

    Techniques: Labeling, Fluorescence, Immunofluorescence

    Effect of exogenous BDNF and NT-4 on neuromuscular transmission: dose–response and time course. A , Dose–response study showing that incubation with BDNF or NT-4 for 1 h does not change EPP size. B , The time course study showing that after 3 h of incubation, both BDNF and NT-4 significantly increased EPP amplitude. Preincubation (1 h) with trkB-IgG completely prevents the potentiating effect (at 3 h) of the neurotrophins. Five muscles and a minimum of 15 fibers per muscle are included for each point. Values are expressed as mean ± SEM. * p

    Journal: The Journal of Neuroscience

    Article Title: The Interaction between Tropomyosin-Related Kinase B Receptors and Presynaptic Muscarinic Receptors Modulates Transmitter Release in Adult Rodent Motor Nerve Terminals

    doi: 10.1523/JNEUROSCI.2676-10.2010

    Figure Lengend Snippet: Effect of exogenous BDNF and NT-4 on neuromuscular transmission: dose–response and time course. A , Dose–response study showing that incubation with BDNF or NT-4 for 1 h does not change EPP size. B , The time course study showing that after 3 h of incubation, both BDNF and NT-4 significantly increased EPP amplitude. Preincubation (1 h) with trkB-IgG completely prevents the potentiating effect (at 3 h) of the neurotrophins. Five muscles and a minimum of 15 fibers per muscle are included for each point. Values are expressed as mean ± SEM. * p

    Article Snippet: Stock solutions used were as follows: anti-BDNF (G2610; Promega), 50 μg/ml; anti-NT-4 (AB1781; Millipore Bioscience Research Reagents), 50 μg/ml; anti-trkB (clone 47/trkB; 610102; BD Transduction Laboratories), 250 μg/ml; human BDNF (Alomone Labs), 0.37 μ m ; human NT-4 (Alomone Labs), 40 n m ; K-252a (Calbiochem), 5 m m in DMSO; K-252b (Calbiochem), 5 m m ; Pep5 (Calbiochem), 100 μ m ; recombinant human trkB/Fc Chimera (trkB-Fc; 688-TK; R & D Systems), 100 μg/ml.

    Techniques: Transmission Assay, Incubation

    ) from confounding interpretation of the data, only pictures obtained from the central retina were compared. Radial sections are shown. A , Control P16 retina. A dopaminergic neuron is indicated by an arrowhead . Very few processes are seen in lamina s3 ( arrowhead ). B , A P16 retina that received three injections of 0.5 μg of NT-3 at P10, P12, and P14. A dopaminergic neuron is indicated by an arrowhead . Labeling is continuous in lamina s1 and less intense in lamina s3 ( small arrows ). C , A P16 retina that received three injections of 0.5 μg of NT-4 at P 10, P12, and P14. A dopaminergic neuron is indicated by an arrowhead . Labeling is continuous in lamina s1 and less intense in lamina s3 ( small arrows ). D , Control P22 retina. A dopaminergic neuron is indicated by an arrowhead . A few processes are seen in lamina s3 ( arrowhead ). E , A P22 retina that received three injections of 1 μg of NT-3 at P16, P18, and P20. A dopaminergic neuron is indicated by an arrowhead . Continuous labeling is observed in lamina s3 ( small arrows ); the labeling in lamina s1 also seems to be increased ( small arrows ). F , A P22 retina that received three injections of 1 μg of NT-4 at P16, P18, and P20. A dopaminergic neuron is indicated by an arrowhead . Continuous labeling is observed in lamina s3 ( small arrows ). Scale bar, 35 μm.

    Journal: The Journal of Neuroscience

    Article Title: Brain-Derived Neurotrophic Factor Modulates the Development of the Dopaminergic Network in the Rodent Retina

    doi: 10.1523/JNEUROSCI.18-09-03351.1998

    Figure Lengend Snippet: ) from confounding interpretation of the data, only pictures obtained from the central retina were compared. Radial sections are shown. A , Control P16 retina. A dopaminergic neuron is indicated by an arrowhead . Very few processes are seen in lamina s3 ( arrowhead ). B , A P16 retina that received three injections of 0.5 μg of NT-3 at P10, P12, and P14. A dopaminergic neuron is indicated by an arrowhead . Labeling is continuous in lamina s1 and less intense in lamina s3 ( small arrows ). C , A P16 retina that received three injections of 0.5 μg of NT-4 at P 10, P12, and P14. A dopaminergic neuron is indicated by an arrowhead . Labeling is continuous in lamina s1 and less intense in lamina s3 ( small arrows ). D , Control P22 retina. A dopaminergic neuron is indicated by an arrowhead . A few processes are seen in lamina s3 ( arrowhead ). E , A P22 retina that received three injections of 1 μg of NT-3 at P16, P18, and P20. A dopaminergic neuron is indicated by an arrowhead . Continuous labeling is observed in lamina s3 ( small arrows ); the labeling in lamina s1 also seems to be increased ( small arrows ). F , A P22 retina that received three injections of 1 μg of NT-4 at P16, P18, and P20. A dopaminergic neuron is indicated by an arrowhead . Continuous labeling is observed in lamina s3 ( small arrows ). Scale bar, 35 μm.

    Article Snippet: One microgram of human recombinant BDNF, human recombinant NT-3, human recombinant NT-4, or mouse β-NGF (Alomone Laboratories) in 2 μl of 0.1% bovine serum albumin (BSA) in sterile PBS was injected with a fine glass microelectrode through the sclera at the level of the temporal peripheral retina.

    Techniques: Labeling