Journal: The Journal of Biological Chemistry
Article Title: Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin
doi: 10.1074/jbc.RA118.001654
Figure Lengend Snippet: Proline-directed serine/threonine CDK substrate motifs in SF3B1 Shown are the proline-directed serine/threonine phosphorylation sites in SF3B1 listed by the PhosphositePlus tool ( 53 ) and previously identified by MS. All serine and threonine residues are shown in lowercase letters. Predicted CDK phosphosites have been underlined. Six threonine residues were mutated to alanine to understand their impact on SF3B1–nucleosome interactions and are denoted by boldface, italicized text.
Article Snippet: Given that CDKs are also known to interact with chromatin and phosphorylate various chromatin-associated proteins ( 26 , 27 ), we hypothesized that phosphorylation of SF3B1 during cell cycle progression regulates its interaction with nucleosomes and in turn could influence cell cycle stage–specific splicing of exons and introns in proximity to SF3B1-bound nucleosomes. table ft1 table-wrap mode="anchored" t5 caption a7 Site Motif Ser-129 II s PE Thr-142 GK t PD Thr-207 DQ t PG Thr-211 GA t PK Thr-223 AE t PG Thr-227 GH t Ps Thr-235 DE t PG Thr-244 a sE t PG Thr-248 a GA t PG Thr-257 DP t Ps Thr-261 sH t PA Thr-267 AA t PG Thr-273 GD t PG Thr-278 HA t PG Thr-296 DE t PK Thr-303 RD t PG Thr-313 a AE t PR Thr-326 GE t Pt Thr-328 tP t PG Thr-341 DE t PA Thr-350 Gs t PV Thr-354 VL t PG Thr-362 IG t PA Thr-369 MA t Pt Thr-371 tP t PG Thr-379 sM t PE Thr-426 IR t PA Thr-434 a tA t Pt Thr-436 tP t PL Ser-488 TL s PE Thr-508 NG t PP Ser-541 LM s PT Thr-1021 RL t PI Thr-1170 AV t PL Open in a separate window a MS data were confirmed in studies utilizing site-specific methods ( 20 , 21 , 34 ).
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