pra 327  (ATCC)

Journal: BMC Microbiology

Article Title: Evidence for methionine-sulfoxide-reductase gene transfer from Alphaproteobacteria to the transcriptionally active (macro)nucleus of the ciliate, Euplotes raikovi

doi: 10.1186/s12866-014-0288-1

Figure Lengend Snippet: Structure of the Euplotes raikovi msrAB gene. Nucleotide sequence: telomeric repetitions, italics; the 5′ and 3′ non-coding regions, lower case letters; coding regions, capital letters; in-frame TGA codons, underlined; ATG, TAA and TAG start and stop codons, shadowed. Deduced amino acid sequence: blue, green and red letters distinguish the putative proteins encoded by the three ORFs.

Article Snippet: Euplotes raikovi cultures used in this study derive from the wild-type strain #13 deposited at the ATCC Center (catalog, #PRA-327), and collected (June 1979) from a sandy coastal site (Porto Recanati, 43° 26′N, 13° 43′E) of the Adriatic cost of Italy [ ].

Techniques: Sequencing

Journal: BMC Microbiology

Article Title: Evidence for methionine-sulfoxide-reductase gene transfer from Alphaproteobacteria to the transcriptionally active (macro)nucleus of the ciliate, Euplotes raikovi

doi: 10.1186/s12866-014-0288-1

Figure Lengend Snippet: Sequence alignment of the Euplotes raikovi MsrA protein (red) encoded by ORF-1 of the msrAB gene with MsrAs of other organisms. MsrAs included in the alignment represent the best hits obtained from prokaryotic and eukaryotic BLASTp searches. Gaps were inserted to maximize alignment, and identical residues are highlighted in gray. Numbers in brackets indicate the percentage of sequence identity of each amino acid sequence with E. raikovi MsrA. Aligned sequences have the following GenBank ID: Thalassobacter arenae , WP_021102447; Sinorhizobium fredii , YP_006401320; Oceanicola sp ., WP_010137233; Ruegeria lacuscaerulensis , WP_005979692; Rhizobium sp ., WP_018236324; Sphingopyxis sp. , WP_003045039; Roseibium sp ., WP_009759924; Pelagibaca bermudensis , WP_007796742; Citreicella sp ., WP_008887323; Nitratireductor aquibiodomus , WP_007008964; Pantholops hodgsonii , XP_005978873; Paramecium tetraurelia , XP_001431627; Tetrahymena thermophila, XP_001020577. Rhodobacterales, green; Rhizobiales, brown; Sphingomonadales, blue; eukaryotic organisms, black.

Article Snippet: Euplotes raikovi cultures used in this study derive from the wild-type strain #13 deposited at the ATCC Center (catalog, #PRA-327), and collected (June 1979) from a sandy coastal site (Porto Recanati, 43° 26′N, 13° 43′E) of the Adriatic cost of Italy [ ].

Techniques: Sequencing

Journal: BMC Microbiology

Article Title: Evidence for methionine-sulfoxide-reductase gene transfer from Alphaproteobacteria to the transcriptionally active (macro)nucleus of the ciliate, Euplotes raikovi

doi: 10.1186/s12866-014-0288-1

Figure Lengend Snippet: Sequence alignment of the 153-amino acid N-terminal region of the Euplotes raikovi MsrB protein (red) encoded by ORF-2 of the msrAB gene with MsrBs of other organisms. The MsrBs included in the alignment represent the best hits obtained from prokaryotic and eukaryotic BLASTp searches. Gaps were inserted to maximize alignment, and identical residues are highlighted in gray. Numbers in brackets indicate the percentage of sequence identity of each amino acid sequence with E. raikovi MsrB. Aligned sequences have the following GenBank ID: Roseovarius nubinhibens , WP_009814088; Roseobacter sp ., WP_007811995; Sinorhizobium meliloti , WP_018098563; Rhizobium sp ., WP_018236325; Nitratireductor aquibiodomus , WP_007008963; Thalassobacter arenae , WP_021102446; Sphingopyxis sp ., WP_003044951; Oceanicola granulosus , WP_007254905; Roseibium sp ., WP_009759925; Mesorhizobium alhagi, WP_008840482; Ruegeria conchae , WP_010442903; Pantholops hodgsonii mitochondrial-like, XP_005955290; Euplotes raikovi , AFZ61875; Paramecium tetraurelia , XP_001426263; Tetrahymena thermophila , XP_001019714. Rhodobacterales, green; Rhizobiales, brown; Sphingomonadales, blue; eukaryotic organisms, black.

Article Snippet: Euplotes raikovi cultures used in this study derive from the wild-type strain #13 deposited at the ATCC Center (catalog, #PRA-327), and collected (June 1979) from a sandy coastal site (Porto Recanati, 43° 26′N, 13° 43′E) of the Adriatic cost of Italy [ ].

Techniques: Sequencing