abeoforma atcc pra  (ATCC)

Journal: Nature Methods

Article Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

doi: 10.1038/s41592-020-0796-x

Figure Lengend Snippet: Parameters used for successful transformation as shown in Figs. 2 , 3 and 4

Article Snippet: Abeoforma whisleri (ATCC PRA-279) , Electroporation Lonza-Nucleofector , 3 × 10 5 , Awhis_H2Bvenus+ pUC19; circular; 1–5 + 40 carrier , Endogenous , Endogenous , NA , 10–15 , 1 , T , Venus , Fluorescence, RT–PCR , http://doi.org/10.17504/protocols.io.zexf3fn.

Techniques: Transformation Assay, Plasmid Preparation, Selection, Electroporation, Fluorescence, Microscopy, Western Blot, Conjugation Assay, Sequencing, Labeling, Staining, Marker, Expressing

Journal: Nature Methods

Article Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

doi: 10.1038/s41592-020-0796-x

Figure Lengend Snippet: a – j , Western blot, RT–PCR or sequencing (in case of Cas9-induced excision by CRISPR) were used to verify expression of introduced constructs in one haptophyte: I. galbana ( a ), one rhizarian— A. amoebiformis ( b ), two stramenopiles— F. cylindrus ( c ) and P. tricornutum ( d ), three alveolates— K. veneficum ( e ), P. marinus ( f ) and A. carterae ( g ), two discobans— B. saltans ( h ) and D. papillatum ( i ) and one opisthokont— A. whisleri ( j ). Note that nptII/neo is used synonymously with amino 3′-glycosyl phosphotransferase gene ( aph (3′)) conferring resistance to kanamycin and neomycin. Representative data of at least two independent experiments are shown. For a detailed figure description see Supplementary Notes .

Article Snippet: Abeoforma whisleri (ATCC PRA-279) , Electroporation Lonza-Nucleofector , 3 × 10 5 , Awhis_H2Bvenus+ pUC19; circular; 1–5 + 40 carrier , Endogenous , Endogenous , NA , 10–15 , 1 , T , Venus , Fluorescence, RT–PCR , http://doi.org/10.17504/protocols.io.zexf3fn.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Sequencing, CRISPR, Expressing, Construct

Journal: Nature Methods

Article Title: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

doi: 10.1038/s41592-020-0796-x

Figure Lengend Snippet: Representative images of transformants and wild-type cell lines of ten selected protist species. Colored boxes behind species names refer to phylogenetic supergroup assignments given in Fig. . Representative data of at least two independent experiments are shown. The fluorescent images show the expression of individual fluorescent marker genes introduced via transformation for all organisms shown, except in the case of A. amoebiformis . For this, red depicts the natural autofluorescence of photosynthetic pigments in the cell, while the additional green spheres in the transformant fluorescence panel shows introduced GFP fluorescence (see Supplementary Fig. for a trace of these different regions in the cell). Scale bars are as follows: 10 µm for A. amoebiformis , T. pseudonana , A. limacinum , B. saltans , N. gruberi , A. whisleri and S. rosetta ; 15 µm for P. marinus ; 20 µm for F. cylindrus and 100 µm for P. multiseries .

Article Snippet: Abeoforma whisleri (ATCC PRA-279) , Electroporation Lonza-Nucleofector , 3 × 10 5 , Awhis_H2Bvenus+ pUC19; circular; 1–5 + 40 carrier , Endogenous , Endogenous , NA , 10–15 , 1 , T , Venus , Fluorescence, RT–PCR , http://doi.org/10.17504/protocols.io.zexf3fn.

Techniques: Expressing, Marker, Transformation Assay, Fluorescence

Journal: PLoS ONE

Article Title: Glycosyltransferase Family 43 Is Also Found in Early Eukaryotes and Has Three Subfamilies in Charophycean Green Algae

doi: 10.1371/journal.pone.0128409

Figure Lengend Snippet: 21 NCBI-nr proteins that are non-plant and non-animal proteins and hit the GT43 domain with an E-value < 1e-10; GenBank identifiers (IDs) having at least 3 motifs are shown in bold and italic.

Article Snippet: EFW41058.2 , 407 , Capsaspora owczarzaki ATCC 30864 , Ichthyosporea , 8.30E-37 , AAI21202.1 , 8.10E-14 , 1.

Techniques: