worthington pds kit  (Worthington Biochemical)


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    Name:
    PDS Kit Inhibitor Vial
    Description:
    Ovomucoid protease inhibitor and bovine serum albumin which is 0 22 micron filtered and lyophilized in autoclaved vials to contain 10 mg ml each upon reconstitution with 32 ml of EBSS
    Catalog Number:
    LK003182
    Price:
    72
    Source:
    Egg White/BSA
    Size:
    1 vi
    Cas Number:
    9035.81.8/9048.46.8
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    Structured Review

    Worthington Biochemical worthington pds kit
    Ovomucoid protease inhibitor and bovine serum albumin which is 0 22 micron filtered and lyophilized in autoclaved vials to contain 10 mg ml each upon reconstitution with 32 ml of EBSS
    https://www.bioz.com/result/worthington pds kit/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    worthington pds kit - by Bioz Stars, 2021-04
    93/100 stars

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    Related Articles

    FACS:

    Article Title: Chronic stress induces sex-specific functional and morphological alterations in cortico-accumbal and cortico-tegmental pathways
    Article Snippet: After 45 minutes of incubation, the tissue was transferred into FACS buffer (5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM-A) and triturated with descending diameter of pipette tips. .. The dissociated cells were then passed through a 70μm filter, placed on an ovomucoid-albumin gradient solution (0.58mg/mL ovomucoid inhibitor-albumin (Worthington Biochemical, LK003182) in FACS buffer) and spun at 900RPM for 6min at 4°C. .. Pelleted cells were then resuspended in 400μL of FACS buffer.

    Article Title: Biology and Bias in Cell Type-Specific RNAseq of Nucleus Accumbens Medium Spiny Neurons
    Article Snippet: Whole cell samples were obtained from fresh tissue, which was rotated at 37 °C for 45 minutes in 1 mg/mL papain suspended in digestion buffer (5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM -A (Thermofisher, A1247501)). .. Tissue was then placed in FACS buffer (0.58 mg/mL albumin inhibitor (Worthington Biochemical, LK003182), 5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM -A) and triturated using progressively smaller pipette tips. .. Samples were passed through a 70 µm filter and placed on top of a layer of 10 mg/mL ovomucoid-albumin in FACS buffer.

    Protease Inhibitor:

    Article Title: Tissue‐ and cell‐specific expression of a splice variant in the II‐III cytoplasmic loop of Cacna1b
    Article Snippet: .. Next, cell suspensions were centrifuged at 300 g for 5 min. After discarding supernatants, pellets were resuspended in 3 mL of EBSS containing 0.1% of ovomucoid protease inhibitor and 0.1% BSA (Worthington, LK003182) to quench papain. ..

    Article Title: Dedifferentiation of committed epithelial cells into stem cells in vivo
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500×g for 5 mins. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. # LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 minutes. .. Cells were then pelleted and stained with EpCAM-PECy7 (1:50; 25-5791-80, eBiosciences) or EpCAM-APC (1:50; 17-5791, eBiosciences); GSIβ4 (Griffonia Simplicifolia Isolectin beta4 )-Biotin (L2120, Sigma); SSEA1 eFluor 650NC (1:75, 95-8813-41, eBiosciences); PE anti-mouse CD24 (1:100, 553262, BD Pharmingen); for 30 min in 2.5% FBS in PBS on ice.

    Article Title: A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500g for 5 min. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. no. LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 min. .. Cells were then pelleted and stained with EpCAM–PECy7 (1:50; 25–5791-80, eBioscience) and CD45, CD81, or basis of GFP expression for 30 min in 2.5% FBS in PBS on ice.

    Transferring:

    Article Title: Biology and Bias in Cell Type-Specific RNAseq of Nucleus Accumbens Medium Spiny Neurons
    Article Snippet: Whole cell samples were obtained from fresh tissue, which was rotated at 37 °C for 45 minutes in 1 mg/mL papain suspended in digestion buffer (5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM -A (Thermofisher, A1247501)). .. Tissue was then placed in FACS buffer (0.58 mg/mL albumin inhibitor (Worthington Biochemical, LK003182), 5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM -A) and triturated using progressively smaller pipette tips. .. Samples were passed through a 70 µm filter and placed on top of a layer of 10 mg/mL ovomucoid-albumin in FACS buffer.

    Incubation:

    Article Title: Dedifferentiation of committed epithelial cells into stem cells in vivo
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500×g for 5 mins. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. # LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 minutes. .. Cells were then pelleted and stained with EpCAM-PECy7 (1:50; 25-5791-80, eBiosciences) or EpCAM-APC (1:50; 17-5791, eBiosciences); GSIβ4 (Griffonia Simplicifolia Isolectin beta4 )-Biotin (L2120, Sigma); SSEA1 eFluor 650NC (1:75, 95-8813-41, eBiosciences); PE anti-mouse CD24 (1:100, 553262, BD Pharmingen); for 30 min in 2.5% FBS in PBS on ice.

    Article Title: Phosphodiesterase 4D acts downstream of Neuropilin to control Hedgehog signal transduction and the growth of medulloblastoma
    Article Snippet: Briefly, cerebella from P7 mice were removed, cut into small pieces with razor blades, and incubated at 37°C for 30 min in digestion buffer consisting of 1x HBSS (ThermoFisher, NY, United States, 14,185) with 10 U/ml papain (Worthington, NJ, United States, LSOO3126) and 250 U/ml DNase (Sigma, MO, United States, D4627). .. At the end of the incubation, the digestion buffer was removed and replaced with 1x HBSS containing 8 mg/ml Ovomucoid (Worthington, NJ, United States, LK003182), 8 mg/ml bovine serum albumin (Sigma), and 250 U/ml DNase. ..

    Article Title: A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500g for 5 min. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. no. LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 min. .. Cells were then pelleted and stained with EpCAM–PECy7 (1:50; 25–5791-80, eBioscience) and CD45, CD81, or basis of GFP expression for 30 min in 2.5% FBS in PBS on ice.

    Article Title: Astragalus polysaccharide attenuates metabolic memory-triggered ER stress and apoptosis via regulation of miR-204/SIRT1 axis in retinal pigment epithelial cells
    Article Snippet: .. The eye was then incubated in a 24-well plate containing 20 U/ml papain solution (Worthington PDS Kit, Lakewood, NJ, U.S.A.) for 1 h at 37°C. ..

    Activity Assay:

    Article Title: Dedifferentiation of committed epithelial cells into stem cells in vivo
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500×g for 5 mins. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. # LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 minutes. .. Cells were then pelleted and stained with EpCAM-PECy7 (1:50; 25-5791-80, eBiosciences) or EpCAM-APC (1:50; 17-5791, eBiosciences); GSIβ4 (Griffonia Simplicifolia Isolectin beta4 )-Biotin (L2120, Sigma); SSEA1 eFluor 650NC (1:75, 95-8813-41, eBiosciences); PE anti-mouse CD24 (1:100, 553262, BD Pharmingen); for 30 min in 2.5% FBS in PBS on ice.

    Article Title: A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
    Article Snippet: After incubation, dissociated tissues were passed through a cell strainer and centrifuged and pelleted at 500g for 5 min. .. Cell pellets were dispersed and incubated with Ovo-mucoid protease inhibitor (Worthington biochemical Corporation, cat. no. LK003182) to inactivate residual papain activity by incubating on a rocker at 4°C for 20 min. .. Cells were then pelleted and stained with EpCAM–PECy7 (1:50; 25–5791-80, eBioscience) and CD45, CD81, or basis of GFP expression for 30 min in 2.5% FBS in PBS on ice.

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  • About
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  • Contact
  • Bioz Stars
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  • 93
    Worthington Biochemical worthington pds kit
    Worthington Pds Kit, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/worthington pds kit/product/Worthington Biochemical
    Average 93 stars, based on 1 article reviews
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    Worthington Biochemical pds kit papain vial
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