gray rjm  (Worthington Biochemical)


Bioz Verified Symbol Worthington Biochemical is a verified supplier
Bioz Manufacturer Symbol Worthington Biochemical manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Name:
    Papain Dissociation System
    Description:
    Set of five single use vials of papain and five single use vials of DNase 100 ml of Earle s balanced salt solution EBSS and an inhibitor vial for use in the tissue dissociation method of Huettner J and Baughman R J Neuroscience 6 3044 1986 Use tested by Worthington using new born rat pup spinal cord The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0 3 0 4 cm3 each
    Catalog Number:
    lk003150
    Price:
    240
    Size:
    1 bx
    Cas Number:
    see components
    Buy from Supplier


    Structured Review

    Worthington Biochemical gray rjm
    Set of five single use vials of papain and five single use vials of DNase 100 ml of Earle s balanced salt solution EBSS and an inhibitor vial for use in the tissue dissociation method of Huettner J and Baughman R J Neuroscience 6 3044 1986 Use tested by Worthington using new born rat pup spinal cord The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0 3 0 4 cm3 each
    https://www.bioz.com/result/gray rjm/product/Worthington Biochemical
    Average 85 stars, based on 1483 article reviews
    Price from $9.99 to $1999.99
    gray rjm - by Bioz Stars, 2020-05
    85/100 stars

    Images

    Related Articles

    Modification:

    Article Title: Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration
    Article Snippet: .. To isolate rod or cone photoreceptors from the retina, we used the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ) following a previously described modified protocol , followed by EasySep Mouse PE or FITC positive selection kit (Stem Cell Technologies, Vancouver, Canada), respectively, following the manufacturer’s protocol. .. We used 1 μg/ml of PE-conjugated anti-CD73 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) ( ) for rod photoreceptors and 5 μg/ml of fluorescein peanut agglutinin (Vector Laboratories, Burlingame, CA) ( ) for cone photoreceptors, respectively.

    Incubation:

    Article Title: Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity
    Article Snippet: .. MOE were dissected into Earl’s Buffered Saline Solution, minced in Papain dissociation solution (Worthington), and incubated at 37 °C for 45 min. ..

    Selection:

    Article Title: Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration
    Article Snippet: .. To isolate rod or cone photoreceptors from the retina, we used the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ) following a previously described modified protocol , followed by EasySep Mouse PE or FITC positive selection kit (Stem Cell Technologies, Vancouver, Canada), respectively, following the manufacturer’s protocol. .. We used 1 μg/ml of PE-conjugated anti-CD73 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) ( ) for rod photoreceptors and 5 μg/ml of fluorescein peanut agglutinin (Vector Laboratories, Burlingame, CA) ( ) for cone photoreceptors, respectively.

    Mouse Assay:

    Article Title: p21Cip restrains hippocampal neurogenesis and protects neuronal progenitors from apoptosis during acute systemic inflammation
    Article Snippet: .. Two-month old WT and p21 -/- mice were sacrificed, the hippocampi dissected and dissociated using Papain Dissociation System (Worthington Biochemical, Lakewood, NJ). .. The NPC cells were isolated and cultured using Neural Stem Cell Expansion Kit Neurosphere System in serum-free neurobasal A-medium (R & D Systems, Minneapolis, MN).

    Article Title: Anti–pan-neurofascin IgG3 as a marker of fulminant autoimmune neuropathy
    Article Snippet: .. Cerebellar granular neurons were prepared from P5 mice: the cerebellum was dissected, and cells were dissociated and suspended using a papain dissociation system (Worthington Biochemical Corporation, Lakewood, NJ). .. Cells were plated on cover slips at a density of 200,000 cells per well and fixed with 4% paraformaldehyde after 3 days, and binding assays with sera of patients and controls were performed at a dilution of 1:500 using an anti-human Cy3-conjugated secondary antibody (Dianova, 1:300).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Worthington Biochemical pds kit inhibitor vial
    Pds Kit Inhibitor Vial, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pds kit inhibitor vial/product/Worthington Biochemical
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pds kit inhibitor vial - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    92
    Worthington Biochemical pd l2
    Impact of <t>RGMb–PD-L2</t> blockade on tolerance induction is not caused by depletion of APC. (a–c) Mice immunized with OVA in ALUM on day 0 received OVA i.n. on days 7–9 and were treated with PD-L2 mAb 2C9 or isotype control (500 µg i.p.) on day 8. Lung cells were dispersed on day 10 and the expression of PD-L2 analyzed on AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and IMs (F4/80 + CD11c lo ). (a) Gating strategy used in b–d. (b) Solid line indicates staining with PD-L2 mAb TY25; shaded histogram indicates isotype control. One mouse/group representative of three is shown. (c) The number of PD-L2 + macrophages and DCs was quantified. Data are representative of two experiments.
    Pd L2, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pd l2/product/Worthington Biochemical
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pd l2 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Worthington Biochemical anti pd 1 dependent human til activation
    Impact of <t>RGMb–PD-L2</t> blockade on tolerance induction is not caused by depletion of APC. (a–c) Mice immunized with OVA in ALUM on day 0 received OVA i.n. on days 7–9 and were treated with PD-L2 mAb 2C9 or isotype control (500 µg i.p.) on day 8. Lung cells were dispersed on day 10 and the expression of PD-L2 analyzed on AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and IMs (F4/80 + CD11c lo ). (a) Gating strategy used in b–d. (b) Solid line indicates staining with PD-L2 mAb TY25; shaded histogram indicates isotype control. One mouse/group representative of three is shown. (c) The number of PD-L2 + macrophages and DCs was quantified. Data are representative of two experiments.
    Anti Pd 1 Dependent Human Til Activation, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pd 1 dependent human til activation/product/Worthington Biochemical
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pd 1 dependent human til activation - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Impact of RGMb–PD-L2 blockade on tolerance induction is not caused by depletion of APC. (a–c) Mice immunized with OVA in ALUM on day 0 received OVA i.n. on days 7–9 and were treated with PD-L2 mAb 2C9 or isotype control (500 µg i.p.) on day 8. Lung cells were dispersed on day 10 and the expression of PD-L2 analyzed on AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and IMs (F4/80 + CD11c lo ). (a) Gating strategy used in b–d. (b) Solid line indicates staining with PD-L2 mAb TY25; shaded histogram indicates isotype control. One mouse/group representative of three is shown. (c) The number of PD-L2 + macrophages and DCs was quantified. Data are representative of two experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Impact of RGMb–PD-L2 blockade on tolerance induction is not caused by depletion of APC. (a–c) Mice immunized with OVA in ALUM on day 0 received OVA i.n. on days 7–9 and were treated with PD-L2 mAb 2C9 or isotype control (500 µg i.p.) on day 8. Lung cells were dispersed on day 10 and the expression of PD-L2 analyzed on AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and IMs (F4/80 + CD11c lo ). (a) Gating strategy used in b–d. (b) Solid line indicates staining with PD-L2 mAb TY25; shaded histogram indicates isotype control. One mouse/group representative of three is shown. (c) The number of PD-L2 + macrophages and DCs was quantified. Data are representative of two experiments.

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Mouse Assay, Expressing, Affinity Magnetic Separation, Staining

    Blockade of PD-L2–RGMb interaction reduces the initial expansion of antigen-specific T cells after intranasal OVA administration. (a) Experimental protocol for Fig. 9 . Mice received DO11.10 T cells on day 0 and OVA i.n. The number of KJ1-26 + cells in mediastinal LNs was determined on the indicated days. (b) Mice treated with RGMb mAb 9D1 or control mAb on day −1 received 5 × 10 6 DO11.10 T cells. Left, TCRβ + KJ1-26 + CD4 + ; Right, FoxP3 + TCRβ + KJ1-26 + CD4 + cells. For each group, mediastinal LNs of 3–5 mice were combined; data are representative of two experiments. (c–e) Mice received 2 × 10 5 DO11.10 T cells after (c) RGMb mAb 9D1 or control mAb; (d) RGMb mAb 9D1, PD-L2 mAb 3.2, or control mAb; (e) PD-L2 mAb 3.2 or control mAb. (f) WT DO11.10 T cells were transferred into WT or PD-L2 −/− mice; (g) WT or PD-L2 −/− DO11.10 T cells were transferred into WT mice. (h–j) Mice received 2 × 10 5 DO11.10 T cells after (h) PD-L2 mAb 2C9 or control mAb; (i) PD-L1 mAb 9G2 or control mAb; (j) PD-L2 mAb 3.2, PD-1 mAb 1A12, or control mAb. The number of KJ1-26 + T cells in mediastinal lymph nodes on day 5 (h–j) was determined by flow cytometry. (b–d) Each point represents a pool of LNs from three mice. Data are representative of two to four experiments. (e–j) Each point represents the mean and SEM of KJ1-26 + T cells in LNs of two or three groups of two mice each. Data are representative of two experiments. **, P

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Blockade of PD-L2–RGMb interaction reduces the initial expansion of antigen-specific T cells after intranasal OVA administration. (a) Experimental protocol for Fig. 9 . Mice received DO11.10 T cells on day 0 and OVA i.n. The number of KJ1-26 + cells in mediastinal LNs was determined on the indicated days. (b) Mice treated with RGMb mAb 9D1 or control mAb on day −1 received 5 × 10 6 DO11.10 T cells. Left, TCRβ + KJ1-26 + CD4 + ; Right, FoxP3 + TCRβ + KJ1-26 + CD4 + cells. For each group, mediastinal LNs of 3–5 mice were combined; data are representative of two experiments. (c–e) Mice received 2 × 10 5 DO11.10 T cells after (c) RGMb mAb 9D1 or control mAb; (d) RGMb mAb 9D1, PD-L2 mAb 3.2, or control mAb; (e) PD-L2 mAb 3.2 or control mAb. (f) WT DO11.10 T cells were transferred into WT or PD-L2 −/− mice; (g) WT or PD-L2 −/− DO11.10 T cells were transferred into WT mice. (h–j) Mice received 2 × 10 5 DO11.10 T cells after (h) PD-L2 mAb 2C9 or control mAb; (i) PD-L1 mAb 9G2 or control mAb; (j) PD-L2 mAb 3.2, PD-1 mAb 1A12, or control mAb. The number of KJ1-26 + T cells in mediastinal lymph nodes on day 5 (h–j) was determined by flow cytometry. (b–d) Each point represents a pool of LNs from three mice. Data are representative of two to four experiments. (e–j) Each point represents the mean and SEM of KJ1-26 + T cells in LNs of two or three groups of two mice each. Data are representative of two experiments. **, P

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry

    Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Blocking capacities of RGMb and PD-L2 mAbs and a model for RGMb and PD-L2 interactions. (a–c) The blocking capacity of RGMb mAbs and PD-L2 mAbs was determined by cell conjugation assay. After cells were stained with dyes, (a) 300-mRGMb cells were incubated with the indicated concentrations of RGMb mAbs before incubation with 300-mPD-L2 cells; (b) 300-mPD-L2 cells were incubated with the indicated concentrations of PD-L2 mAbs before incubation with 300-mRGMb cells; or (c) before incubation with 300-mPD-1 cells. (d) mRGMb-HIS was preincubated with mAbs or Ig fusion proteins and then added to BMP-4–coated plates. Binding of mRGMb-HIS was detected by anti–penta-HIS-HRP in an ELISA. Similar results were seen with BMP-2 (not depicted). (e) mPD-L2-hIgG or control-hIgG were preincubated alone or with monomeric mRGMb-HIS, and then added to BMP-4–coated plates. Binding of Ig fusion proteins was detected with anti–hIgG-HRP in an ELISA. Similar results were also seen for BMP-2 (not depicted). (f) mNeogenin- or mPD-L2–transfected 300 cells were stained with indicated Ig fusion proteins or control-Ig and analyzed by flow cytometry. (g) Binding of 300-mRGMb cells to 300-mPD-L2 or 300-mNeogenin cells was analyzed by cell conjugation assay. Cells stained with a red dye were incubated with cells stained with a green dye and binding was measured by flow cytometry. (h) Molecular model depicting PDL2–BMP–BMPR–RGMb–neogenin and PD-L2–PD-1 pathways. All data are representative of two or more independent experiments.

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Blocking Assay, Conjugation Assay, Staining, Incubation, Binding Assay, Enzyme-linked Immunosorbent Assay, Transfection, Flow Cytometry, Cytometry

    Expression of RGMb, PD-L2, BMPs, BMPRs, and related molecules in resting lung cell populations and airway epithelial cells. Expression of indicated mRNAs in resting lung cell populations and airway epithelial cells by qRT-PCR. (a–h) IMs (F4/80 + CD11c − ), AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and other cells (F4/80 − CD11c − ) were sorted from lung cells pooled from five to eight mice; CD4 + T cells (TCRβ + CD4 + ) and CD8 + T cells (TCRβ + CD8 + ) were sorted from lung cells pooled from two mice. (i) AECs and TECs were pooled from three and eight mice, respectively. Data are mean ± SEM; n = 3; *, P

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: Expression of RGMb, PD-L2, BMPs, BMPRs, and related molecules in resting lung cell populations and airway epithelial cells. Expression of indicated mRNAs in resting lung cell populations and airway epithelial cells by qRT-PCR. (a–h) IMs (F4/80 + CD11c − ), AMs (F4/80 + CD11c + ), DCs (F4/80 − CD11c + ), and other cells (F4/80 − CD11c − ) were sorted from lung cells pooled from five to eight mice; CD4 + T cells (TCRβ + CD4 + ) and CD8 + T cells (TCRβ + CD8 + ) were sorted from lung cells pooled from two mice. (i) AECs and TECs were pooled from three and eight mice, respectively. Data are mean ± SEM; n = 3; *, P

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Expressing, Quantitative RT-PCR, Affinity Magnetic Separation, Mouse Assay

    RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: RGMb binds to PD-L2, but not PD-L1 or other related molecules. (a and b) mRGMb- or mPD-L2–transfected 300 cells, untransfected 300 cells, and PD-1–transfected Jurkat T cells were stained with the indicated Ig fusion proteins (red) or control-Ig (blue) and analyzed by flow cytometry. MFI, mean fluorescence intensity. (c and d) Binding of mPD-L2-Ig or control-Ig to plates coated with mRGMb-HIS, hRGMb-HIS, mRGMa-HIS or mRGMc-HIS, was analyzed by ELISA. (e) Biacore analysis of the interaction of RGMb with PD-L2 and of PD-1 with PD-L2. (f–k) Cell–cell binding of the indicated transfected cells was analyzed by cell conjugation assay. In f, h, and j, the top panels show the FSC-SSC plots and the bottom panels show the corresponding dot plots. g, i, and k show only the dot plots. All data are representative of 2–11 independent experiments.

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Transfection, Staining, Flow Cytometry, Cytometry, Fluorescence, Binding Assay, Enzyme-linked Immunosorbent Assay, Conjugation Assay

    PD-L2 or RGMb blockade inhibits induction of respiratory tolerance. (a) Experimental protocol for induction of respiratory tolerance. (b–d) PD-L2–deficient or WT mice in b, and WT mice injected with the indicated mAb in c and d, were exposed to OVA i.n. (tolerized) or PBS (nontolerized) and subsequently received 50 µg OVA/ALUM i.p. T cell proliferation (top) and IL-4 production (bottom) in response to restimulation with OVA in vitro are shown. n = 2–3. Data are representative of two to five experiments. Data are mean ± SEM, *, P

    Journal: The Journal of Experimental Medicine

    Article Title: RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance

    doi: 10.1084/jem.20130790

    Figure Lengend Snippet: PD-L2 or RGMb blockade inhibits induction of respiratory tolerance. (a) Experimental protocol for induction of respiratory tolerance. (b–d) PD-L2–deficient or WT mice in b, and WT mice injected with the indicated mAb in c and d, were exposed to OVA i.n. (tolerized) or PBS (nontolerized) and subsequently received 50 µg OVA/ALUM i.p. T cell proliferation (top) and IL-4 production (bottom) in response to restimulation with OVA in vitro are shown. n = 2–3. Data are representative of two to five experiments. Data are mean ± SEM, *, P

    Article Snippet: To induce tolerance, lightly anesthetized WT BALB/cByJ or PD-L2−/− mice received 100 µg of LPS-free OVA (Worthington Biochemical) in PBS or PBS alone (control) by intranasal instillation on days 0, 1, and 2.

    Techniques: Mouse Assay, Injection, In Vitro