Journal: Translational Vision Science & Technology

Article Title: The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells

doi: 10.1167/tvst.11.9.16

Figure Lengend Snippet: Representative pictures of cultured primary corneal epithelial cells at critical time points after the start of drug application. Phase contrast pictures of primary cells 24 hours after start of drug application with (A) untreated control, (B) BSS for 24 hours, (C) cyclosporine for 24 hours, (D) lifitegrast for 4 hours, and (E) tacrolimus for 1 hour. Note that BSS did not affect survival of cells but did alter transparency of the cells. Cells treated with 10% tested drug, 90% normal growth medium. Scale bar : 50 µm.

Article Snippet: The tissue was centrifuged at 1000 revolutions per minute for 5 minutes, and the cells were suspended in 1 mL corneal epithelial cell medium (PCS-700-030; ATCC) supplemented with corneal epithelial cell growth kit medium (PCS-700-040; ATCC), 33-µM Phenol red (P0290-100ML; MilliporeSigma), and 0.1× Antibiotic-Antimycotic (15240062; Thermo Fisher Scientific, Waltham, MA).

Techniques: Cell Culture

Journal: Translational Vision Science & Technology

Article Title: The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells

doi: 10.1167/tvst.11.9.16

Figure Lengend Snippet: Immortalized human corneal epithelial cells morphology following anti-inflammatory treatments. Brightfield images of iHCE cells 72 hours following treatments with saline (A, F, K, P), cyclosporine (B, G, L, Q), lifitegrast (C, H, M, R), tacrolimus 0.03% (D, I, N, S), and tacrolimus 0.1% (E, J, O, T). Treatment times: 30 seconds (A–E), 1 minute (F–J), 1 hour (K–O), and 4 hours (P–T). Cells treated with 20% tested drug, 80% normal growth medium. Scale bar : 200 µm.

Article Snippet: The tissue was centrifuged at 1000 revolutions per minute for 5 minutes, and the cells were suspended in 1 mL corneal epithelial cell medium (PCS-700-030; ATCC) supplemented with corneal epithelial cell growth kit medium (PCS-700-040; ATCC), 33-µM Phenol red (P0290-100ML; MilliporeSigma), and 0.1× Antibiotic-Antimycotic (15240062; Thermo Fisher Scientific, Waltham, MA).

Techniques:

Journal: Translational Vision Science & Technology

Article Title: The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells

doi: 10.1167/tvst.11.9.16

Figure Lengend Snippet: Effect of dry eye drugs on primary epithelial cell cultures from corneal transplant donor tissue. Testing eye drops in a separate primary cell line for validation using a lower concentration and longer treatment time (compare with Fig. 1 ). Primary epithelial cell cultures were treated with (A) BSS as control, (B) tacrolimus 0.1%, (C) lifitegrast, and (D) cyclosporine for 2 minutes, 1 hour, 2 hours, 4 hours, and 24 hours. Cell survival was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay after 48 hours.

Article Snippet: Cells obtained from ATCC were treated according to the manufacturer's instructions with Corneal Epithelial Cell Basal Medium (PCS-700-030; ATACC) and Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC).

Techniques: Concentration Assay, Cell Viability Assay

Journal: Translational Vision Science & Technology

Article Title: The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells

doi: 10.1167/tvst.11.9.16

Figure Lengend Snippet: Representative pictures of cultured primary corneal epithelial cells at critical time points after the start of drug application. Phase contrast pictures of primary cells 24 hours after start of drug application with (A) untreated control, (B) BSS for 24 hours, (C) cyclosporine for 24 hours, (D) lifitegrast for 4 hours, and (E) tacrolimus for 1 hour. Note that BSS did not affect survival of cells but did alter transparency of the cells. Cells treated with 10% tested drug, 90% normal growth medium. Scale bar : 50 µm.

Article Snippet: Cells obtained from ATCC were treated according to the manufacturer's instructions with Corneal Epithelial Cell Basal Medium (PCS-700-030; ATACC) and Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC).

Techniques: Cell Culture

Journal: Translational Vision Science & Technology

Article Title: The Effect of Anti-Inflammatory Topical Ophthalmic Treatments on In Vitro Corneal Epithelial Cells

doi: 10.1167/tvst.11.9.16

Figure Lengend Snippet: Immortalized human corneal epithelial cells morphology following anti-inflammatory treatments. Brightfield images of iHCE cells 72 hours following treatments with saline (A, F, K, P), cyclosporine (B, G, L, Q), lifitegrast (C, H, M, R), tacrolimus 0.03% (D, I, N, S), and tacrolimus 0.1% (E, J, O, T). Treatment times: 30 seconds (A–E), 1 minute (F–J), 1 hour (K–O), and 4 hours (P–T). Cells treated with 20% tested drug, 80% normal growth medium. Scale bar : 200 µm.

Article Snippet: Cells obtained from ATCC were treated according to the manufacturer's instructions with Corneal Epithelial Cell Basal Medium (PCS-700-030; ATACC) and Corneal Epithelial Cell Growth Kit (PCS-700-040; ATCC).

Techniques: