Journal: Bioengineered

Article Title: MicroRNA-15b-5p inhibits tumor necrosis factor alpha-induced proliferation, migration, and extracellular matrix production of airway smooth muscle cells via targeting yes-associated protein 1

doi: 10.1080/21655979.2022.2036890

Figure Lengend Snippet: The effects of TNF-α on miR-15b-5p expression in ASM cells.

Article Snippet: Human primary ASM cells (ATCC PCS-130-011™, Manassas, VA, USA) were subsequently cultured in Dulbecco’s modified Eagles medium/Ham’s F12 (Hyclone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA), 100 unit/mL penicillin, and 100 μg/mL streptomycin (Cellgro, Manassas, VA, USA).

Techniques: Expressing

Journal: Bioengineered

Article Title: MicroRNA-15b-5p inhibits tumor necrosis factor alpha-induced proliferation, migration, and extracellular matrix production of airway smooth muscle cells via targeting yes-associated protein 1

doi: 10.1080/21655979.2022.2036890

Figure Lengend Snippet: The effects of miR-15b-5p on TNF-α-induced proliferation and migration of ASM cells.

Article Snippet: Human primary ASM cells (ATCC PCS-130-011™, Manassas, VA, USA) were subsequently cultured in Dulbecco’s modified Eagles medium/Ham’s F12 (Hyclone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA), 100 unit/mL penicillin, and 100 μg/mL streptomycin (Cellgro, Manassas, VA, USA).

Techniques: Migration

Journal: Bioengineered

Article Title: MicroRNA-15b-5p inhibits tumor necrosis factor alpha-induced proliferation, migration, and extracellular matrix production of airway smooth muscle cells via targeting yes-associated protein 1

doi: 10.1080/21655979.2022.2036890

Figure Lengend Snippet: The effects of miR-15b-5p on TNF-α-induced inflammatory response and ECM deposition in ASM cells.

Article Snippet: Human primary ASM cells (ATCC PCS-130-011™, Manassas, VA, USA) were subsequently cultured in Dulbecco’s modified Eagles medium/Ham’s F12 (Hyclone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA), 100 unit/mL penicillin, and 100 μg/mL streptomycin (Cellgro, Manassas, VA, USA).

Techniques:

Journal: Bioengineered

Article Title: MicroRNA-15b-5p inhibits tumor necrosis factor alpha-induced proliferation, migration, and extracellular matrix production of airway smooth muscle cells via targeting yes-associated protein 1

doi: 10.1080/21655979.2022.2036890

Figure Lengend Snippet: The restoration of YAP1 expression reverses the effect of the upregulated miR-15b-5p on ASM cells.

Article Snippet: Human primary ASM cells (ATCC PCS-130-011™, Manassas, VA, USA) were subsequently cultured in Dulbecco’s modified Eagles medium/Ham’s F12 (Hyclone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA), 100 unit/mL penicillin, and 100 μg/mL streptomycin (Cellgro, Manassas, VA, USA).

Techniques: Expressing

Journal: Molecular and Cellular Biology

Article Title: The Activin/FLRG Pathway Associates with Poor COVID-19 Outcomes in Hospitalized Patients

doi: 10.1128/MCB.00467-21

Figure Lengend Snippet: Both IL-1α and TNF-α can stimulate activin A production in bronchial/tracheal SMCs, lung fibroblasts and pulmonary artery SMCs, which can be rescued by dexamethasone. Activin A production was measured in conditioned medium by enzyme-linked immunosorbent assay (ELISA). Bronchial/tracheal smooth muscle (BTSMC) cells, normal human lung fibroblasts (NHLF), and pulmonary artery smooth muscle cells (PASMC) were serum starved overnight and then treated with 10 ng/ml IL-1 α or TNF-α for 5 days in the presence or absence of 100 μM dexamethasone, 100 nM anti-activin A antibody REGN2477 or isotype control REGN1945. Conditioned medium was diluted at 1:10 for ELISA. The experiment was repeated twice, with a consistent result.

Article Snippet: Bronchial/tracheal smooth muscle cells (BTSMC; ATCC, PCS-130-011), normal human lung fibroblasts (NHLF; Lonza, CC-2512), and pulmonary artery smooth muscle cells (PASMC; Lonza, CC-2581) were seeded at 200,000 cells/well in 12-well plates and cultured in media as specified by manufacturers.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Biology

Article Title: The Activin/FLRG Pathway Associates with Poor COVID-19 Outcomes in Hospitalized Patients

doi: 10.1128/MCB.00467-21

Figure Lengend Snippet: Role of IKK, p38 and JNK in activin A induction in response to cytokine treatment. Bronchial/tracheal smooth muscle cells (SMC) (top) pulmonary artery SMC (bottom) were treated for 24 h with 100 ng/ml of TNF-α or IL-1α in combination with pharmacological inhibitors for IKK (3 μM withaferin A), p38 (0.3 μM SB203580) and JNK (30 μM SP600125). A combination of IKK and p38 inhibitors (same concentrations), a combination of IKK and JNK inhibitors (same concentrations) and a combination of p38 and JNK inhibitors (same concentrations) was also used. DMSO was used as a negative control for inhibitor treatments. Treatment with sterile water only (Mock) was used as an additional control. Activin A levels were quantified in conditioned media by ELISA. n = 3 for each treatment. Significant Bonferroni-corrected pairwise comparisons with DMSO within each treatment condition are labeled. * , P < 0.05, * * , P < 0.01, ** * , P < 0.001, *** * , P < 0.0001.

Article Snippet: Bronchial/tracheal smooth muscle cells (BTSMC; ATCC, PCS-130-011), normal human lung fibroblasts (NHLF; Lonza, CC-2512), and pulmonary artery smooth muscle cells (PASMC; Lonza, CC-2581) were seeded at 200,000 cells/well in 12-well plates and cultured in media as specified by manufacturers.

Techniques: Negative Control, Enzyme-linked Immunosorbent Assay, Labeling